The papain inhibitor (SPI) of Streptomyces mobaraensis inhibits bacterial cysteine proteases and is an antagonist of bacterial growth

Stephan Zindel; Wendy E Kaman; Sabrina Fröls; Felicitas Pfeifer; Anna Peters; John P Hays; Hans-Lothar Fuchsbauer

doi:10.1128/AAC.00129-13

The papain inhibitor (SPI) of Streptomyces mobaraensis inhibits bacterial cysteine proteases and is an antagonist of bacterial growth

Stephan Zindel, Wendy E Kaman, Sabrina Fröls, Felicitas Pfeifer, Anna Peters, John P Hays, Hans-Lothar Fuchsbauer

Oral Biochemistry

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

A novel papain inhibitory protein (SPI) from Streptomyces mobaraensis was studied to measure its inhibitory effect on bacterial cysteine protease activity (Staphylococcus aureus SspB) and culture supernatants (Porphyromonas gingivalis, Bacillus anthracis). Further, growth of Bacillus anthracis, Staphylococcus aureus, Pseudomonas aeruginosa, and Vibrio cholerae was completely inhibited by 10 μM SPI. At this concentration of SPI, no cytotoxicity was observed. We conclude that SPI inhibits bacterial virulence factors and has the potential to become a novel therapeutic treatment against a range of unrelated pathogenic bacteria.

Original language	English
Pages (from-to)	3388-91
Number of pages	4
Journal	Antimicrobial agents and chemotherapy
Volume	57
Issue number	7
DOIs	https://doi.org/10.1128/AAC.00129-13
Publication status	Published - Jul 2013

Keywords

Bacillus anthracis/drug effects
Bacterial Proteins/metabolism
Cysteine Proteases/metabolism
Cysteine Proteinase Inhibitors/pharmacology
Papain/antagonists & inhibitors
Porphyromonas gingivalis/drug effects
Pseudomonas aeruginosa/drug effects
Staphylococcus aureus/drug effects
Streptomyces/chemistry
Vibrio cholerae/drug effects

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10.1128/AAC.00129-13

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title = "The papain inhibitor (SPI) of Streptomyces mobaraensis inhibits bacterial cysteine proteases and is an antagonist of bacterial growth",

abstract = "A novel papain inhibitory protein (SPI) from Streptomyces mobaraensis was studied to measure its inhibitory effect on bacterial cysteine protease activity (Staphylococcus aureus SspB) and culture supernatants (Porphyromonas gingivalis, Bacillus anthracis). Further, growth of Bacillus anthracis, Staphylococcus aureus, Pseudomonas aeruginosa, and Vibrio cholerae was completely inhibited by 10 μM SPI. At this concentration of SPI, no cytotoxicity was observed. We conclude that SPI inhibits bacterial virulence factors and has the potential to become a novel therapeutic treatment against a range of unrelated pathogenic bacteria.",

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author = "Stephan Zindel and Kaman, {Wendy E} and Sabrina Fr{\"o}ls and Felicitas Pfeifer and Anna Peters and Hays, {John P} and Hans-Lothar Fuchsbauer",

year = "2013",

month = jul,

doi = "10.1128/AAC.00129-13",

language = "English",

volume = "57",

pages = "3388--91",

journal = "Antimicrobial agents and chemotherapy",

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T1 - The papain inhibitor (SPI) of Streptomyces mobaraensis inhibits bacterial cysteine proteases and is an antagonist of bacterial growth

AU - Zindel, Stephan

AU - Kaman, Wendy E

AU - Fröls, Sabrina

AU - Pfeifer, Felicitas

AU - Peters, Anna

AU - Hays, John P

AU - Fuchsbauer, Hans-Lothar

PY - 2013/7

Y1 - 2013/7

N2 - A novel papain inhibitory protein (SPI) from Streptomyces mobaraensis was studied to measure its inhibitory effect on bacterial cysteine protease activity (Staphylococcus aureus SspB) and culture supernatants (Porphyromonas gingivalis, Bacillus anthracis). Further, growth of Bacillus anthracis, Staphylococcus aureus, Pseudomonas aeruginosa, and Vibrio cholerae was completely inhibited by 10 μM SPI. At this concentration of SPI, no cytotoxicity was observed. We conclude that SPI inhibits bacterial virulence factors and has the potential to become a novel therapeutic treatment against a range of unrelated pathogenic bacteria.

AB - A novel papain inhibitory protein (SPI) from Streptomyces mobaraensis was studied to measure its inhibitory effect on bacterial cysteine protease activity (Staphylococcus aureus SspB) and culture supernatants (Porphyromonas gingivalis, Bacillus anthracis). Further, growth of Bacillus anthracis, Staphylococcus aureus, Pseudomonas aeruginosa, and Vibrio cholerae was completely inhibited by 10 μM SPI. At this concentration of SPI, no cytotoxicity was observed. We conclude that SPI inhibits bacterial virulence factors and has the potential to become a novel therapeutic treatment against a range of unrelated pathogenic bacteria.

KW - Bacillus anthracis/drug effects

KW - Bacterial Proteins/metabolism

KW - Cysteine Proteases/metabolism

KW - Cysteine Proteinase Inhibitors/pharmacology

KW - Papain/antagonists & inhibitors

KW - Porphyromonas gingivalis/drug effects

KW - Pseudomonas aeruginosa/drug effects

KW - Staphylococcus aureus/drug effects

KW - Streptomyces/chemistry

KW - Vibrio cholerae/drug effects

U2 - 10.1128/AAC.00129-13

DO - 10.1128/AAC.00129-13

M3 - Article

C2 - 23587952

SN - 0066-4804

VL - 57

SP - 3388

EP - 3391

JO - Antimicrobial agents and chemotherapy

JF - Antimicrobial agents and chemotherapy

IS - 7

ER -

The papain inhibitor (SPI) of Streptomyces mobaraensis inhibits bacterial cysteine proteases and is an antagonist of bacterial growth

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