The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII

Lauren Nicol, Roberta Croce*

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Photosynthesis is tightly regulated in order to withstand dynamic light environments. Under high light intensities, a mechanism known as non-photochemical quenching (NPQ) dissipates excess excitation energy, protecting the photosynthetic machinery from damage. An obstacle that lies in the way of understanding the molecular mechanism of NPQ is the large gap between in vitro and in vivo studies. On the one hand, the complexity of the photosynthetic membrane makes it challenging to obtain molecular information from in vivo experiments. On the other hand, a suitable in vitro system for the study of quenching is not available. Here we have developed a minimal NPQ system using proteoliposomes. With this, we demonstrate that the combination of low pH and PsbS is both necessary and sufficient to induce quenching in LHCII, the main antenna complex of plants. This proteoliposome system can be further exploited to gain more insight into how PsbS and other factors (e.g. zeaxanthin) influence the quenching mechanism observed in LHCII.

Original languageEnglish
Article number7415
Pages (from-to)1-8
Number of pages8
JournalScientific Reports
Volume11
Issue number1
Early online date1 Apr 2021
DOIs
Publication statusE-pub ahead of print - 1 Apr 2021

Bibliographical note

Funding Information:
The authors thank Laura M. Roy for constructing the PsbS-Strep plants and Vincent M. Friebe for helpful technical advice. The project was supported by the Dutch organization for Scientific research via a VICI Grant to R.C. L.N. received financial support from the New Zealand Government through the Royal Society of New Zealand Te Apārangi Rutherford Foundation.

Publisher Copyright:
© 2021, The Author(s).

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

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