The relation between blood lactate and ammonia in ischemic handgrip exercise

Sytze P T Sinkeler, Hein A M Daanen, Ron A. Wevers, T. Lian Oei, Ed M G Joosten, Rob A. Binkhorst*

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review


Subjects with myopathies associated with certain enzyme defects often show abnormal concentrations of lactate (LA) and ammonia (NH3) in blood after ischemic exercise. Myoadenylate deaminase (MAD) deficient patients produce only small amounts of NH3, whereas LA rises to normal levels. On the other hand, patients with certain enzyme deficiencies in the glyco(geno)lytic pathway show the opposite. However, the concentrations in blood are dependent on the exercise performed. Standardization of tests for screening purposes, therefore, is necessary. For ischemic contractions, experiments were performed to find the optimal combination for force and frequency, using the highest LA and NH3 concentrations in blood as criteria. Eleven healthy subjects performed ischemic isometric contractions with a handgrip dynamometer at frequencies of 30 and 50/min−1 and force levels of 50%, 65%, and 80% of maximal voluntary contraction (MVC). A combination of 30/min−1 and 80% MVC was found to give the best results.

Original languageEnglish
Pages (from-to)523-527
Number of pages5
JournalMuscle and Nerve
Issue number6
Publication statusPublished - 1985


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