The Relation between Oral Candida Load and Bacterial Microbiome Profiles in Dutch Older Adults.

E.A. Kraneveld; M.J. Buijs; M.J. Bonder; M. Visser; B.J. Keijser; W. Crielaard; E. Zaura

doi:10.1371/journal.pone.0042770

The Relation between Oral Candida Load and Bacterial Microbiome Profiles in Dutch Older Adults.

E.A. Kraneveld, M.J. Buijs, M.J. Bonder, M. Visser, B.J. Keijser, W. Crielaard, E. Zaura

Nutrition and Health

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Abstract

Currently there are no evidence-based ecological measures for prevention of overgrowth and subsequent infection by fungi in the oral cavity. The aim of this study was to increase our knowledge on fungal-bacterial ecological interactions. Salivary Candida abundance of 82 Dutch adults aged 58-80 years was established relative to the bacterial load by quantitative PCR analysis of the Internal Transcribed (ITS) region (Candida) and 16S rDNA gene (bacteria). The salivary microbiome was assessed using barcoded pyrosequencing of the bacterial hypervariable regions V5-V7 of 16S rDNA. Sequencing data was preprocessed by denoising and chimera removal, clustered in Operational Taxonomic Units (OTUs) and assigned to taxonomy. Both OTU-based (PCA, diversity statistics) and phylogeny-based analyses (UniFrac, PCoA) were performed. Saliva of Dutch older adults contained 0-4 × 10

Original language	English
Article number	e42770
Journal	PLoS ONE
Volume	7
Issue number	8
Early online date	10 Aug 2012
DOIs	https://doi.org/10.1371/journal.pone.0042770
Publication status	Published - 2012

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abstract = "Currently there are no evidence-based ecological measures for prevention of overgrowth and subsequent infection by fungi in the oral cavity. The aim of this study was to increase our knowledge on fungal-bacterial ecological interactions. Salivary Candida abundance of 82 Dutch adults aged 58-80 years was established relative to the bacterial load by quantitative PCR analysis of the Internal Transcribed (ITS) region (Candida) and 16S rDNA gene (bacteria). The salivary microbiome was assessed using barcoded pyrosequencing of the bacterial hypervariable regions V5-V7 of 16S rDNA. Sequencing data was preprocessed by denoising and chimera removal, clustered in Operational Taxonomic Units (OTUs) and assigned to taxonomy. Both OTU-based (PCA, diversity statistics) and phylogeny-based analyses (UniFrac, PCoA) were performed. Saliva of Dutch older adults contained 0-4 × 10",

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AU - Visser, M.

AU - Keijser, B.J.

AU - Crielaard, W.

AU - Zaura, E.

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The Relation between Oral Candida Load and Bacterial Microbiome Profiles in Dutch Older Adults.

Abstract

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