Abstract
Background: Gut bacteria play a crucial role in the metabolism of bile acids (BA). Whether an association exists between the fecal microbiota composition and circulating BA levels in humans is poorly understood. Here, we investigated the relationship between fecal microbiota diversity and composition with plasma levels of BA in young adults. Methods: Fecal microbiota diversity/composition was analyzed with 16S rRNA sequencing in 80 young adults (74% women; 21.9 ± 2.2 years old). Plasma levels of BA were measured using liquid chromatography-tandem mass spectrometry. PERMANOVA and Spearman correlation analyses were used to investigate the association between fecal microbiota parameters and plasma levels of BA. Results: Fecal microbiota beta (P = 0.025) and alpha diversity indexes of evenness (rho = 0.237, P = 0.033), Shannon (rho = 0.313, P = 0.004), and inverse Simpson (rho = 0.283, P = 0.010) were positively associated with plasma levels of the secondary BA glycolithocholic acid (GLCA). The relative abundance of genera belonging to the Firmicutes and Bacteroidetes phyla was positively correlated with plasma levels of GLCA (all rho ≥ 0.225, P ≤ 0.049). However, the relative abundance of species from Firmicutes and Bacteroidetes phyla were negatively correlated with plasma levels of primary and secondary BA (all rho ≤ − 0.220, P ≤ 0.045), except for the relative abundance of Bacteroides vulgatus, Alistipes onderdonkii, and Bacteroides xylanisolvens species (Bacteroidetes phylum) that were positively correlated with the plasma levels of GLCA. Conclusions: The relative abundance of specific fecal bacteria species is associated with plasma levels of BA in young adults. However, further investigations are required to validate whether the composition of the gut microbiota can regulate the plasma concentrations of BA in humans.
Original language | English |
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Article number | 54 |
Pages (from-to) | 1-12 |
Number of pages | 12 |
Journal | Metabolomics |
Volume | 19 |
Issue number | 6 |
Early online date | 6 Jun 2023 |
DOIs | |
Publication status | Published - Jun 2023 |
Bibliographical note
Funding Information:Funding for open access publishing: Universidad de Granada/CBUA. The study was supported by the Spanish Ministry of Economy and Competitiveness via Fondo de Investigación Sanitaria del Instituto de Salud Carlos III (PI13/01393) and PTA 12264-I, Retos de la Sociedad (DEP2016-79512-R), and European Regional Development Funds (ERDF), by the Spanish Ministry of Education (FPU16/05159, FPU16/02828, FPU17/01523 and FPU19/01609), the Fundación Iberoamericana de Nutrición (FINUT), the University of Granada Plan Propio de Investigación 2016-Excellence actions: Unit of Excellence on Exercise and Health (UCEES), AstraZeneca HealthCare Foundation, and by the Junta de Andalucía, Consejería de Economía, Conocimiento, Empresas y Universidad (ref. P18-RT-4455), the Chinese Scholarship Council (CSC, No. 201707060012 to XD), Fundación Alfonso Martin Escudero and Maria Zambrano fellowship by the Ministerio de Universidades y la Unión Europea –NextGenerationEU (RR_C_2021_04). We would like to thank the team of Data Integration Center of University Medicine Magdeburg for local data-analysis solutions; they are supported by MIRACUM and funded by the German Federal Ministry of Education and Research (BMBF) within the “Medical Informatics Funding Scheme” (FKZ 01ZZ1801H). This study is part of a Ph.D. thesis conducted within the Biomedicine Doctoral Studies Program of the University of Granada, Spain. AL was supported by the funds from European Commission through the “European funds for regional development” (EFRE) Project ID: ZS/2018/11/95324.
Publisher Copyright:
© 2023, The Author(s).
Funding
Funding for open access publishing: Universidad de Granada/CBUA. The study was supported by the Spanish Ministry of Economy and Competitiveness via Fondo de Investigación Sanitaria del Instituto de Salud Carlos III (PI13/01393) and PTA 12264-I, Retos de la Sociedad (DEP2016-79512-R), and European Regional Development Funds (ERDF), by the Spanish Ministry of Education (FPU16/05159, FPU16/02828, FPU17/01523 and FPU19/01609), the Fundación Iberoamericana de Nutrición (FINUT), the University of Granada Plan Propio de Investigación 2016-Excellence actions: Unit of Excellence on Exercise and Health (UCEES), AstraZeneca HealthCare Foundation, and by the Junta de Andalucía, Consejería de Economía, Conocimiento, Empresas y Universidad (ref. P18-RT-4455), the Chinese Scholarship Council (CSC, No. 201707060012 to XD), Fundación Alfonso Martin Escudero and Maria Zambrano fellowship by the Ministerio de Universidades y la Unión Europea –NextGenerationEU (RR_C_2021_04). We would like to thank the team of Data Integration Center of University Medicine Magdeburg for local data-analysis solutions; they are supported by MIRACUM and funded by the German Federal Ministry of Education and Research (BMBF) within the “Medical Informatics Funding Scheme” (FKZ 01ZZ1801H). This study is part of a Ph.D. thesis conducted within the Biomedicine Doctoral Studies Program of the University of Granada, Spain. AL was supported by the funds from European Commission through the “European funds for regional development” (EFRE) Project ID: ZS/2018/11/95324.
Funders | Funder number |
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CBUA | |
MIRACUM | |
Ministerio de Universidades y la Unión Europea | RR_C_2021_04 |
University of Granada Plan Propio de Investigación | |
Fundación Alfonso Martín Escudero | |
Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía | P18-RT-4455 |
Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía | |
AstraZeneca HealthCare Foundation | |
Fundación Iberoamericana de Nutrición | |
European Commission | |
Bundesministerium für Bildung und Forschung | FKZ 01ZZ1801H |
Bundesministerium für Bildung und Forschung | |
Ministerio de Educación, Cultura y Deporte | FPU16/05159, FPU16/02828, FPU17/01523, FPU19/01609 |
Ministerio de Educación, Cultura y Deporte | |
Ministerio de Economía y Competitividad | |
China Scholarship Council | 201707060012 |
China Scholarship Council | |
Instituto de Salud Carlos III | PI13/01393, DEP2016-79512-R, PTA 12264-I |
Instituto de Salud Carlos III | |
Universidad de Granada | |
European Regional Development Fund | ZS/2018/11/95324 |
European Regional Development Fund |
Keywords
- 7-α-Dehydroxylases
- Bile salt hydrolases
- Gut microbiota
- Microbiome