Abstract
Metabolomics is concerned with the comprehensive analysis of endogenous low-molecular-weight compounds in biological samples. Capillary electrophoresis-mass spectrometry (CE-MS) has demonstrated to be a powerful technique for the profiling of polar and charged metabolites in biological samples. In this chapter we describe the most recent developments in CE-MS for metabolomics, including both targeted and nontargeted approaches. This chapter covers the use of various CE separation modes, capillary coatings, CE-MS interfacing, sample pretreatment, and data analysis methods used in CE-MS for metabolomics. The applicability of CE-MS in metabolomics research is illustrated by examples of the analysis of various biological samples. Future considerations such as the use of sheathless CE-MS interfacing and challenges for large-scale and (quantitative) clinical metabolomics studies are discussed. CE-MS-based metabolomics studies reported between January 2006 and June 2011 and information on sample type and pretreatment and MS detection mode are summarized in tables.
| Original language | English |
|---|---|
| Title of host publication | Metabolomics in Practice |
| Subtitle of host publication | Successful Strategies to Generate and Analyze Metabolic Data |
| Editors | M Laemmerhofer, W. Weckwerth |
| Place of Publication | Weinheim |
| Publisher | Wiley-VCH |
| Pages | 177-208 |
| Number of pages | 32 |
| ISBN (Print) | 9783527330898 |
| DOIs | |
| Publication status | Published - 2013 |
Keywords
- Blood
- Capillary electrophoresis-mass spectrometry
- Data analysis
- Metabolomics
- Nontargeted
- Plant extract
- Sample pretreatment
- Targeted
- Urine
