The scavenging capacity of DMBT1 is impaired by germline deletions

F.J. Bikker; C. End; A.J.M. Ligtenberg; S. Blaich; S. Lyer; M. Renner; R. Wittig; K. Nazmi; A. van Nieuw Amerongen; A. Poustka; E.C.I. Veerman; J. Mollenhauer

doi:10.1007/s00251-017-0982-x

The scavenging capacity of DMBT1 is impaired by germline deletions

F.J. Bikker
, C. End
, A.J.M. Ligtenberg
, S. Blaich
, S. Lyer
, M. Renner
, R. Wittig
, K. Nazmi
, A. van Nieuw Amerongen
, A. Poustka
, E.C.I. Veerman
, J. Mollenhauer

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

The Scavenger Receptor Cysteine-Rich (SRCR) proteins are an archaic group of proteins characterized by the presence of multiple SRCR domains. They are membrane-bound or secreted proteins, which are generally related to host defense systems in animals. Deleted in Malignant Brain Tumors 1 (DMBT1) is a SRCR protein which is secreted in mucosal fluids and involved in host defense by pathogen binding by its SRCR domains. Genetic polymorphism within DMBT1 leads to DMBT1-alleles giving rise to polypeptides with interindividually different numbers of SRCR domains, ranging from 8 SRCR domains (encoded by 6 kb DMBT1 variant) to 13 SRCR domains (encoded by the 8 kb DMBT1 variant). In the present study, we have investigated whether reduction from 13 to 8 amino-terminal SRCR domains leads to reduction of bacterial binding. The 6 kb variant bound ~20–45% less bacteria compared to the 8 kb variant. These results support the hypothesis that genetic variation in DMBT1 may influence microbial defense.

Original language	English
Pages (from-to)	401-407
Journal	Immunogenetics
Volume	69
Issue number	6
DOIs	https://doi.org/10.1007/s00251-017-0982-x
Publication status	Published - Jun 2017

Funding

This work was supported by the European Molecular Biology Organization (EMBO), grant ASTF 115-02, the Netherlands Organization for Scientific Research (NWO), grant ER 90-184, the Deutsche Krebshilfe, grant no. 1835-Mo I, and the Wilhelm Sander-Stiftung, grant no. 99.018.2.

Funders	Funder number
European Molecular Biology Organization	ASTF 115-02
Wilhelm Sander-Stiftung	99.018.2
Nederlandse Organisatie voor Wetenschappelijk Onderzoek	ER 90-184
Deutsche Krebshilfe	1835-Mo I

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https://hdl.handle.net/11245.1/eb0e2e62-65d8-4864-8f32-fce8e13c677a

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title = "The scavenging capacity of DMBT1 is impaired by germline deletions",

abstract = "The Scavenger Receptor Cysteine-Rich (SRCR) proteins are an archaic group of proteins characterized by the presence of multiple SRCR domains. They are membrane-bound or secreted proteins, which are generally related to host defense systems in animals. Deleted in Malignant Brain Tumors 1 (DMBT1) is a SRCR protein which is secreted in mucosal fluids and involved in host defense by pathogen binding by its SRCR domains. Genetic polymorphism within DMBT1 leads to DMBT1-alleles giving rise to polypeptides with interindividually different numbers of SRCR domains, ranging from 8 SRCR domains (encoded by 6 kb DMBT1 variant) to 13 SRCR domains (encoded by the 8 kb DMBT1 variant). In the present study, we have investigated whether reduction from 13 to 8 amino-terminal SRCR domains leads to reduction of bacterial binding. The 6 kb variant bound \textasciitilde{}20–45\% less bacteria compared to the 8 kb variant. These results support the hypothesis that genetic variation in DMBT1 may influence microbial defense.",

author = "F.J. Bikker and C. End and A.J.M. Ligtenberg and S. Blaich and S. Lyer and M. Renner and R. Wittig and K. Nazmi and \{van Nieuw Amerongen\}, A. and A. Poustka and E.C.I. Veerman and J. Mollenhauer",

year = "2017",

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doi = "10.1007/s00251-017-0982-x",

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AU - Bikker, F.J.

AU - End, C.

AU - Ligtenberg, A.J.M.

AU - Blaich, S.

AU - Lyer, S.

AU - Renner, M.

AU - Wittig, R.

AU - Nazmi, K.

AU - van Nieuw Amerongen, A.

AU - Poustka, A.

AU - Veerman, E.C.I.

AU - Mollenhauer, J.

PY - 2017/6

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AB - The Scavenger Receptor Cysteine-Rich (SRCR) proteins are an archaic group of proteins characterized by the presence of multiple SRCR domains. They are membrane-bound or secreted proteins, which are generally related to host defense systems in animals. Deleted in Malignant Brain Tumors 1 (DMBT1) is a SRCR protein which is secreted in mucosal fluids and involved in host defense by pathogen binding by its SRCR domains. Genetic polymorphism within DMBT1 leads to DMBT1-alleles giving rise to polypeptides with interindividually different numbers of SRCR domains, ranging from 8 SRCR domains (encoded by 6 kb DMBT1 variant) to 13 SRCR domains (encoded by the 8 kb DMBT1 variant). In the present study, we have investigated whether reduction from 13 to 8 amino-terminal SRCR domains leads to reduction of bacterial binding. The 6 kb variant bound ~20–45% less bacteria compared to the 8 kb variant. These results support the hypothesis that genetic variation in DMBT1 may influence microbial defense.

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JO - Immunogenetics

JF - Immunogenetics

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The scavenging capacity of DMBT1 is impaired by germline deletions

Abstract

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