The Supramolecular Organization of a Peptide-Based Nanocarrier at High Molecular Detail

Mazda Rad-Malekshahi, Koen M. Visscher, João P.G.L.M. Rodrigues, Renko De Vries, Wim E. Hennink, Marc Baldus, Alexandre M.J.J. Bonvin, Enrico Mastrobattista, Markus Weingarth

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review

Abstract

Nanovesicles self-assembled from amphiphilic peptides are promising candidates for applications in drug delivery. However, complete high-resolution data on the local and supramolecular organization of such materials has been elusive thus far, which is a substantial obstacle to their rational design. In the absence of precise information, nanovesicles built of amphiphilic “lipid-like” peptides are generally assumed to resemble liposomes that are organized from bilayers of peptides with a tail-to-tail ordering. Using the nanocarrier formed by the amphiphilic self-assembling peptide 2 (SA2 peptide) as an example, we derive the local and global organization of a multimega-Dalton peptide-based nanocarrier at high molecular detail and at close-to physiological conditions. By integrating a multitude of experimental techniques (solid-state NMR, AFM, SLS, DLS, FT-IR, CD) with large- and multiscale MD simulations, we show that SA2 nanocarriers are built of interdigitated antiparallel β-sheets, which bear little res...
Original languageEnglish
Title of host publicationJournal of the American Chemical Society
PublisherAmerican Chemical Society
Pages7775-7784
Number of pages10
Volume137
Edition24
ISBN (Print)0002-7863
DOIs
Publication statusPublished - 24 Jun 2015

Publication series

NameJournal of the American Chemical Society
Volume137

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