The systemic control of circadian gene expression

A. Gerber, C. Saini, T. Curie, Y. Emmenegger, G. Rando, P. Gosselin, I. Gotic, P. Gos, P. Franken, U. Schibler

Research output: Contribution to JournalReview articleAcademicpeer-review

Abstract

The mammalian circadian timing system consists of a central pacemaker in the brain's suprachiasmatic nucleus (SCN) and subsidiary oscillators in nearly all body cells. The SCN clock, which is adjusted to geophysical time by the photoperiod, synchronizes peripheral clocks through a wide variety of systemic cues. The latter include signals depending on feeding cycles, glucocorticoid hormones, rhythmic blood-borne signals eliciting daily changes in actin dynamics and serum response factor (SRF) activity, and sensors of body temperature rhythms, such as heat shock transcription factors and the cold-inducible RNA-binding protein CIRP. To study these systemic signalling pathways, we designed and engineered a novel, highly photosensitive apparatus, dubbed RT-Biolumicorder. This device enables us to record circadian luciferase reporter gene expression in the liver and other organs of freely moving mice over months in real time. Owing to the multitude of systemic signalling pathway involved in the phase resetting of peripheral clocks the disruption of any particular one has only minor effects on the steady state phase of circadian gene expression in organs such as the liver. Nonetheless, the implication of specific pathways in the synchronization of clock gene expression can readily be assessed by monitoring the phase-shifting kinetics using the RT-Biolumicorder.

Original languageEnglish
Pages (from-to)23-32
Number of pages10
JournalDiabetes, Obesity and Metabolism
Volume17
Issue numberS1
DOIs
Publication statusPublished - 1 Sep 2015

Fingerprint

Suprachiasmatic Nucleus
Gene Expression
Serum Response Factor
Circadian Clocks
RNA-Binding Proteins
Liver
Photoperiod
Body Temperature
Luciferases
Reporter Genes
Glucocorticoids
Cues
Actins
Hormones
Equipment and Supplies
Brain
heat shock transcription factor

Keywords

  • Actin dynamics
  • Body temperature rhythms
  • Circadian clock
  • Feeding rhythms
  • Glucocorticoid hormones
  • Peripheral oscillators
  • Serum response factor (SRF)
  • Synchronization

Cite this

Gerber, A., Saini, C., Curie, T., Emmenegger, Y., Rando, G., Gosselin, P., ... Schibler, U. (2015). The systemic control of circadian gene expression. Diabetes, Obesity and Metabolism, 17(S1), 23-32. https://doi.org/10.1111/dom.12512
Gerber, A. ; Saini, C. ; Curie, T. ; Emmenegger, Y. ; Rando, G. ; Gosselin, P. ; Gotic, I. ; Gos, P. ; Franken, P. ; Schibler, U. / The systemic control of circadian gene expression. In: Diabetes, Obesity and Metabolism. 2015 ; Vol. 17, No. S1. pp. 23-32.
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Gerber, A, Saini, C, Curie, T, Emmenegger, Y, Rando, G, Gosselin, P, Gotic, I, Gos, P, Franken, P & Schibler, U 2015, 'The systemic control of circadian gene expression' Diabetes, Obesity and Metabolism, vol. 17, no. S1, pp. 23-32. https://doi.org/10.1111/dom.12512

The systemic control of circadian gene expression. / Gerber, A.; Saini, C.; Curie, T.; Emmenegger, Y.; Rando, G.; Gosselin, P.; Gotic, I.; Gos, P.; Franken, P.; Schibler, U.

In: Diabetes, Obesity and Metabolism, Vol. 17, No. S1, 01.09.2015, p. 23-32.

Research output: Contribution to JournalReview articleAcademicpeer-review

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T1 - The systemic control of circadian gene expression

AU - Gerber, A.

AU - Saini, C.

AU - Curie, T.

AU - Emmenegger, Y.

AU - Rando, G.

AU - Gosselin, P.

AU - Gotic, I.

AU - Gos, P.

AU - Franken, P.

AU - Schibler, U.

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AB - The mammalian circadian timing system consists of a central pacemaker in the brain's suprachiasmatic nucleus (SCN) and subsidiary oscillators in nearly all body cells. The SCN clock, which is adjusted to geophysical time by the photoperiod, synchronizes peripheral clocks through a wide variety of systemic cues. The latter include signals depending on feeding cycles, glucocorticoid hormones, rhythmic blood-borne signals eliciting daily changes in actin dynamics and serum response factor (SRF) activity, and sensors of body temperature rhythms, such as heat shock transcription factors and the cold-inducible RNA-binding protein CIRP. To study these systemic signalling pathways, we designed and engineered a novel, highly photosensitive apparatus, dubbed RT-Biolumicorder. This device enables us to record circadian luciferase reporter gene expression in the liver and other organs of freely moving mice over months in real time. Owing to the multitude of systemic signalling pathway involved in the phase resetting of peripheral clocks the disruption of any particular one has only minor effects on the steady state phase of circadian gene expression in organs such as the liver. Nonetheless, the implication of specific pathways in the synchronization of clock gene expression can readily be assessed by monitoring the phase-shifting kinetics using the RT-Biolumicorder.

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KW - Feeding rhythms

KW - Glucocorticoid hormones

KW - Peripheral oscillators

KW - Serum response factor (SRF)

KW - Synchronization

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U2 - 10.1111/dom.12512

DO - 10.1111/dom.12512

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JO - Diabetes, Obesity and Metabolism

JF - Diabetes, Obesity and Metabolism

SN - 1462-8902

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Gerber A, Saini C, Curie T, Emmenegger Y, Rando G, Gosselin P et al. The systemic control of circadian gene expression. Diabetes, Obesity and Metabolism. 2015 Sep 1;17(S1):23-32. https://doi.org/10.1111/dom.12512