TY - JOUR
T1 - The systemic control of circadian gene expression
AU - Gerber, A.
AU - Saini, C.
AU - Curie, T.
AU - Emmenegger, Y.
AU - Rando, G.
AU - Gosselin, P.
AU - Gotic, I.
AU - Gos, P.
AU - Franken, P.
AU - Schibler, U.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - The mammalian circadian timing system consists of a central pacemaker in the brain's suprachiasmatic nucleus (SCN) and subsidiary oscillators in nearly all body cells. The SCN clock, which is adjusted to geophysical time by the photoperiod, synchronizes peripheral clocks through a wide variety of systemic cues. The latter include signals depending on feeding cycles, glucocorticoid hormones, rhythmic blood-borne signals eliciting daily changes in actin dynamics and serum response factor (SRF) activity, and sensors of body temperature rhythms, such as heat shock transcription factors and the cold-inducible RNA-binding protein CIRP. To study these systemic signalling pathways, we designed and engineered a novel, highly photosensitive apparatus, dubbed RT-Biolumicorder. This device enables us to record circadian luciferase reporter gene expression in the liver and other organs of freely moving mice over months in real time. Owing to the multitude of systemic signalling pathway involved in the phase resetting of peripheral clocks the disruption of any particular one has only minor effects on the steady state phase of circadian gene expression in organs such as the liver. Nonetheless, the implication of specific pathways in the synchronization of clock gene expression can readily be assessed by monitoring the phase-shifting kinetics using the RT-Biolumicorder.
AB - The mammalian circadian timing system consists of a central pacemaker in the brain's suprachiasmatic nucleus (SCN) and subsidiary oscillators in nearly all body cells. The SCN clock, which is adjusted to geophysical time by the photoperiod, synchronizes peripheral clocks through a wide variety of systemic cues. The latter include signals depending on feeding cycles, glucocorticoid hormones, rhythmic blood-borne signals eliciting daily changes in actin dynamics and serum response factor (SRF) activity, and sensors of body temperature rhythms, such as heat shock transcription factors and the cold-inducible RNA-binding protein CIRP. To study these systemic signalling pathways, we designed and engineered a novel, highly photosensitive apparatus, dubbed RT-Biolumicorder. This device enables us to record circadian luciferase reporter gene expression in the liver and other organs of freely moving mice over months in real time. Owing to the multitude of systemic signalling pathway involved in the phase resetting of peripheral clocks the disruption of any particular one has only minor effects on the steady state phase of circadian gene expression in organs such as the liver. Nonetheless, the implication of specific pathways in the synchronization of clock gene expression can readily be assessed by monitoring the phase-shifting kinetics using the RT-Biolumicorder.
KW - Actin dynamics
KW - Body temperature rhythms
KW - Circadian clock
KW - Feeding rhythms
KW - Glucocorticoid hormones
KW - Peripheral oscillators
KW - Serum response factor (SRF)
KW - Synchronization
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U2 - 10.1111/dom.12512
DO - 10.1111/dom.12512
M3 - Review article
C2 - 26332965
AN - SCOPUS:84940563299
SN - 1462-8902
VL - 17
SP - 23
EP - 32
JO - Diabetes, Obesity and Metabolism
JF - Diabetes, Obesity and Metabolism
IS - S1
ER -