TY - JOUR
T1 - The targets of 2,2',5,5'-tetrachlorobiphenyl in the respiratory chain of rat liver mitochondria revealed by modular kinetic analysis.
AU - Mildaziene, V.
AU - Nauciene, Z.
AU - Krab, K.
PY - 2002
Y1 - 2002
N2 - The response of the respiratory subsystem of oxidative phosphorylation to the environmental pollutant, 2,2′,5,5′-tetrachlorobiphenyl (2,2′,5,5′-TCB) was investigated by modular kinetic approach. The effects of 20 μM 2,2′,5,5′-TCB on the activity of the respiratory chain modules in rat liver mitochondria oxidizing succinate (+ rotenone) in state 3 were assessed. The toxin inhibited the rate of respiration by 23%. Analysis around cytochrome c revealed that 2,2′,5,5′-TCB inhibited both cytochrome c-oxidizing and - reducing modules. The toxin inhibited also CoQ-oxidizing module, however it did not affect the kinetics of CoQ-reducing module. Taken together, these data indicated that 2,2′,5,5′-TCB inhibited cytochrome bc
AB - The response of the respiratory subsystem of oxidative phosphorylation to the environmental pollutant, 2,2′,5,5′-tetrachlorobiphenyl (2,2′,5,5′-TCB) was investigated by modular kinetic approach. The effects of 20 μM 2,2′,5,5′-TCB on the activity of the respiratory chain modules in rat liver mitochondria oxidizing succinate (+ rotenone) in state 3 were assessed. The toxin inhibited the rate of respiration by 23%. Analysis around cytochrome c revealed that 2,2′,5,5′-TCB inhibited both cytochrome c-oxidizing and - reducing modules. The toxin inhibited also CoQ-oxidizing module, however it did not affect the kinetics of CoQ-reducing module. Taken together, these data indicated that 2,2′,5,5′-TCB inhibited cytochrome bc
U2 - 10.1023/A:1020394016372
DO - 10.1023/A:1020394016372
M3 - Article
SN - 0301-4851
VL - 29
SP - 31
EP - 34
JO - Molecular Biology Reports
JF - Molecular Biology Reports
ER -
