The targets of 2,2',5,5'-tetrachlorobiphenyl in the respiratory chain of rat liver mitochondria revealed by modular kinetic analysis.

V. Mildaziene, Z. Nauciene, K. Krab

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

The response of the respiratory subsystem of oxidative phosphorylation to the environmental pollutant, 2,2′,5,5′-tetrachlorobiphenyl (2,2′,5,5′-TCB) was investigated by modular kinetic approach. The effects of 20 μM 2,2′,5,5′-TCB on the activity of the respiratory chain modules in rat liver mitochondria oxidizing succinate (+ rotenone) in state 3 were assessed. The toxin inhibited the rate of respiration by 23%. Analysis around cytochrome c revealed that 2,2′,5,5′-TCB inhibited both cytochrome c-oxidizing and - reducing modules. The toxin inhibited also CoQ-oxidizing module, however it did not affect the kinetics of CoQ-reducing module. Taken together, these data indicated that 2,2′,5,5′-TCB inhibited cytochrome bc
Original languageEnglish
Pages (from-to)31-34
JournalMolecular Biology Reports
Volume29
DOIs
Publication statusPublished - 2002

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