The two-component signal transduction protein Chk1p regulates quorum sensing in Candida albicans

Michael Kruppa; Bastiaan P Krom; Neeraj Chauhan; Adrienne V Bambach; Ronald L Cihlar; Richard A Calderone

doi:10.1128/EC.3.4.1062-1065.2004

The two-component signal transduction protein Chk1p regulates quorum sensing in Candida albicans

Michael Kruppa, Bastiaan P Krom, Neeraj Chauhan, Adrienne V Bambach, Ronald L Cihlar, Richard A Calderone

Preventive Dentistry

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Regulation of hyphal morphogenesis in Candida albicans can occur through quorum sensing (QS). A QS signal, farnesol, is produced during high-density growth and inhibits morphogenesis. However, the signal transduction pathway that regulates QS is unknown. Here, we show that a C. albicans mutant lacking Chk1p but not either the Sln1p or the Nik1p histidine kinase is refractory to the inhibitory effect of farnesol both in cell suspension and during the formation of a biofilm. This study is the first to demonstrate a role for a two-component signal transduction protein in QS by a eukaryotic organism.

Original language	English
Pages (from-to)	1062-5
Number of pages	4
Journal	Eukaryotic Cell
Volume	3
Issue number	4
DOIs	https://doi.org/10.1128/EC.3.4.1062-1065.2004
Publication status	Published - Aug 2004

Keywords

Biofilms
Candida albicans/cytology
Checkpoint Kinase 1
Farnesol/metabolism
Fungal Proteins/genetics
Morphogenesis/physiology
Protein Kinases/genetics
Signal Transduction/physiology

Access to Document

10.1128/EC.3.4.1062-1065.2004

Persistent URL (handle)

Persistent link

Cite this

@article{d956a1a84db1415bb67718f99d26b460,

title = "The two-component signal transduction protein Chk1p regulates quorum sensing in Candida albicans",

abstract = "Regulation of hyphal morphogenesis in Candida albicans can occur through quorum sensing (QS). A QS signal, farnesol, is produced during high-density growth and inhibits morphogenesis. However, the signal transduction pathway that regulates QS is unknown. Here, we show that a C. albicans mutant lacking Chk1p but not either the Sln1p or the Nik1p histidine kinase is refractory to the inhibitory effect of farnesol both in cell suspension and during the formation of a biofilm. This study is the first to demonstrate a role for a two-component signal transduction protein in QS by a eukaryotic organism.",

keywords = "Biofilms, Candida albicans/cytology, Checkpoint Kinase 1, Farnesol/metabolism, Fungal Proteins/genetics, Morphogenesis/physiology, Protein Kinases/genetics, Signal Transduction/physiology",

author = "Michael Kruppa and Krom, {Bastiaan P} and Neeraj Chauhan and Bambach, {Adrienne V} and Cihlar, {Ronald L} and Calderone, {Richard A}",

year = "2004",

month = aug,

doi = "10.1128/EC.3.4.1062-1065.2004",

language = "English",

volume = "3",

pages = "1062--5",

journal = "Eukaryotic Cell",

issn = "1535-9778",

publisher = "American Society for Microbiology",

number = "4",

}

TY - JOUR

T1 - The two-component signal transduction protein Chk1p regulates quorum sensing in Candida albicans

AU - Kruppa, Michael

AU - Krom, Bastiaan P

AU - Chauhan, Neeraj

AU - Bambach, Adrienne V

AU - Cihlar, Ronald L

AU - Calderone, Richard A

PY - 2004/8

Y1 - 2004/8

N2 - Regulation of hyphal morphogenesis in Candida albicans can occur through quorum sensing (QS). A QS signal, farnesol, is produced during high-density growth and inhibits morphogenesis. However, the signal transduction pathway that regulates QS is unknown. Here, we show that a C. albicans mutant lacking Chk1p but not either the Sln1p or the Nik1p histidine kinase is refractory to the inhibitory effect of farnesol both in cell suspension and during the formation of a biofilm. This study is the first to demonstrate a role for a two-component signal transduction protein in QS by a eukaryotic organism.

AB - Regulation of hyphal morphogenesis in Candida albicans can occur through quorum sensing (QS). A QS signal, farnesol, is produced during high-density growth and inhibits morphogenesis. However, the signal transduction pathway that regulates QS is unknown. Here, we show that a C. albicans mutant lacking Chk1p but not either the Sln1p or the Nik1p histidine kinase is refractory to the inhibitory effect of farnesol both in cell suspension and during the formation of a biofilm. This study is the first to demonstrate a role for a two-component signal transduction protein in QS by a eukaryotic organism.

KW - Biofilms

KW - Candida albicans/cytology

KW - Checkpoint Kinase 1

KW - Farnesol/metabolism

KW - Fungal Proteins/genetics

KW - Morphogenesis/physiology

KW - Protein Kinases/genetics

KW - Signal Transduction/physiology

U2 - 10.1128/EC.3.4.1062-1065.2004

DO - 10.1128/EC.3.4.1062-1065.2004

M3 - Article

C2 - 15302838

SN - 1535-9778

VL - 3

SP - 1062

EP - 1065

JO - Eukaryotic Cell

JF - Eukaryotic Cell

IS - 4

ER -

The two-component signal transduction protein Chk1p regulates quorum sensing in Candida albicans

Abstract

Keywords

Access to Document

Persistent URL (handle)

Fingerprint

Cite this