The xanthophylls in light-harvesting complex II of higher plants: light harvesting and triplet quenching.

E.J.G. Peterman, C.C. Gradinaru, F. Calkoen, J.C. Borst, R. van Grondelle, H. van Amerongen

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

A spectral and functional assignment of the xanthophylls in monomeric and trimeric light-harvesting complex II of green plants has been obtained using HPLC analysis of the pigment composition, laser-flash induced triplet- minus-singlet, fluorescence excitation, and absorption spectra. It is shown that violaxanthin is not present in monomeric preparations, that it has most likely a red-most absorption maximum at 510 nm in the trimeric complex, and that it is involved in both light-harvesting and Chl-triplet quenching. Two xanthophylls (per monomer) have an absorption maximum at 494 nm. These play a major role in both singlet and triplet transfer. These two are most probably the two xanthophylls resolved in the crystal structure, tentatively assigned to lutein, that are close to several chlorophyll molecules [Kuhlbrandt, W., Wang, N., D., and Fujiyoshi, Y. (1994) Nature 367, 614-621]. A last xanthophyll contribution, with an absorption maximum at 486 nm, does not seem to play a significant role in light-harvesting or in Chl-triplet quenching. On the basis of the assumption that the two structurally resolved xanthophylls are lutein, this 486 nm absorbing xanthophyll should be neoxanthin. The measurements demonstrate that violaxanthin is connected to at least one chlorophyll a with an absorption maximum near 670 nm, whereas the xanthophylls absorbing at 494 nm are connected to at least one chlorophyll a with a peak near 675 nm.
Original languageEnglish
Pages (from-to)12208-12215
JournalBiochemistry
Volume36
DOIs
Publication statusPublished - 1997

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Xanthophylls
Quenching
Light
Lutein
Viridiplantae
Chlorophyll
Pigments
Absorption spectra
Lasers
Monomers
Crystal structure
Fluorescence
High Pressure Liquid Chromatography
Molecules
Chemical analysis

Cite this

Peterman, E.J.G. ; Gradinaru, C.C. ; Calkoen, F. ; Borst, J.C. ; van Grondelle, R. ; van Amerongen, H. / The xanthophylls in light-harvesting complex II of higher plants: light harvesting and triplet quenching. In: Biochemistry. 1997 ; Vol. 36. pp. 12208-12215.
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abstract = "A spectral and functional assignment of the xanthophylls in monomeric and trimeric light-harvesting complex II of green plants has been obtained using HPLC analysis of the pigment composition, laser-flash induced triplet- minus-singlet, fluorescence excitation, and absorption spectra. It is shown that violaxanthin is not present in monomeric preparations, that it has most likely a red-most absorption maximum at 510 nm in the trimeric complex, and that it is involved in both light-harvesting and Chl-triplet quenching. Two xanthophylls (per monomer) have an absorption maximum at 494 nm. These play a major role in both singlet and triplet transfer. These two are most probably the two xanthophylls resolved in the crystal structure, tentatively assigned to lutein, that are close to several chlorophyll molecules [Kuhlbrandt, W., Wang, N., D., and Fujiyoshi, Y. (1994) Nature 367, 614-621]. A last xanthophyll contribution, with an absorption maximum at 486 nm, does not seem to play a significant role in light-harvesting or in Chl-triplet quenching. On the basis of the assumption that the two structurally resolved xanthophylls are lutein, this 486 nm absorbing xanthophyll should be neoxanthin. The measurements demonstrate that violaxanthin is connected to at least one chlorophyll a with an absorption maximum near 670 nm, whereas the xanthophylls absorbing at 494 nm are connected to at least one chlorophyll a with a peak near 675 nm.",
author = "E.J.G. Peterman and C.C. Gradinaru and F. Calkoen and J.C. Borst and {van Grondelle}, R. and {van Amerongen}, H.",
year = "1997",
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The xanthophylls in light-harvesting complex II of higher plants: light harvesting and triplet quenching. / Peterman, E.J.G.; Gradinaru, C.C.; Calkoen, F.; Borst, J.C.; van Grondelle, R.; van Amerongen, H.

In: Biochemistry, Vol. 36, 1997, p. 12208-12215.

Research output: Contribution to JournalArticleAcademicpeer-review

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T1 - The xanthophylls in light-harvesting complex II of higher plants: light harvesting and triplet quenching.

AU - Peterman, E.J.G.

AU - Gradinaru, C.C.

AU - Calkoen, F.

AU - Borst, J.C.

AU - van Grondelle, R.

AU - van Amerongen, H.

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N2 - A spectral and functional assignment of the xanthophylls in monomeric and trimeric light-harvesting complex II of green plants has been obtained using HPLC analysis of the pigment composition, laser-flash induced triplet- minus-singlet, fluorescence excitation, and absorption spectra. It is shown that violaxanthin is not present in monomeric preparations, that it has most likely a red-most absorption maximum at 510 nm in the trimeric complex, and that it is involved in both light-harvesting and Chl-triplet quenching. Two xanthophylls (per monomer) have an absorption maximum at 494 nm. These play a major role in both singlet and triplet transfer. These two are most probably the two xanthophylls resolved in the crystal structure, tentatively assigned to lutein, that are close to several chlorophyll molecules [Kuhlbrandt, W., Wang, N., D., and Fujiyoshi, Y. (1994) Nature 367, 614-621]. A last xanthophyll contribution, with an absorption maximum at 486 nm, does not seem to play a significant role in light-harvesting or in Chl-triplet quenching. On the basis of the assumption that the two structurally resolved xanthophylls are lutein, this 486 nm absorbing xanthophyll should be neoxanthin. The measurements demonstrate that violaxanthin is connected to at least one chlorophyll a with an absorption maximum near 670 nm, whereas the xanthophylls absorbing at 494 nm are connected to at least one chlorophyll a with a peak near 675 nm.

AB - A spectral and functional assignment of the xanthophylls in monomeric and trimeric light-harvesting complex II of green plants has been obtained using HPLC analysis of the pigment composition, laser-flash induced triplet- minus-singlet, fluorescence excitation, and absorption spectra. It is shown that violaxanthin is not present in monomeric preparations, that it has most likely a red-most absorption maximum at 510 nm in the trimeric complex, and that it is involved in both light-harvesting and Chl-triplet quenching. Two xanthophylls (per monomer) have an absorption maximum at 494 nm. These play a major role in both singlet and triplet transfer. These two are most probably the two xanthophylls resolved in the crystal structure, tentatively assigned to lutein, that are close to several chlorophyll molecules [Kuhlbrandt, W., Wang, N., D., and Fujiyoshi, Y. (1994) Nature 367, 614-621]. A last xanthophyll contribution, with an absorption maximum at 486 nm, does not seem to play a significant role in light-harvesting or in Chl-triplet quenching. On the basis of the assumption that the two structurally resolved xanthophylls are lutein, this 486 nm absorbing xanthophyll should be neoxanthin. The measurements demonstrate that violaxanthin is connected to at least one chlorophyll a with an absorption maximum near 670 nm, whereas the xanthophylls absorbing at 494 nm are connected to at least one chlorophyll a with a peak near 675 nm.

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