Thermostating in capillary electrophoresis.

J.R. Veraart, C. Gooijer, H. Lingeman

Research output: Contribution to JournalArticleAcademicpeer-review


The use of high voltages across a electrophoresis capillary will increase the temperature of the buffer due to Joule heating. As a result temperature control in CE is rather important since variations in the buffer temperature will result in changes in the pi-I of the buffer, peak shape, migration time, reproducibility, efficiency, 3-D structure of macromolecular analytes, etc. Six different thermostating systems have been evaluated: (i) natural convection, (ii) fan, (iii) home-made and (iv and v) two commercially available high-speed air and a (vi) liquid thermostated device. In all cases the temperature of the buffer in the capillary is calculated according to the temperature-conductivity relationship. For this purpose two parameters are introduced describing temperature control: the temperature onset (δT) and the temperature rise factor (a). From these results, it can be concluded that high speed air thermostating can be as efficient as liquid thermostating.
Original languageEnglish
Pages (from-to)129
Issue number3/4
Publication statusPublished - 1997


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