Third harmonic generation imaging for fast, label-free pathology of human brain tumors

N.V. Kuzmin, P. Wesseling, P.C.D. Hamer, D.P. Noske, G. Galgano, H.D. Mansvelder, J.C. Baayen, M.L. Groot

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

In brain tumor surgery, recognition of tumor boundaries is key. However, intraoperative assessment of tumor boundaries by the neurosurgeon is difficult. Therefore, there is an urgent need for tools that provide the neurosurgeon with pathological information during the operation. We show that third harmonic generation (THG) microscopy provides label-free, real-time images of histopathological quality; increased cellularity, nuclear pleomorphism, and rarefaction of neuropil in fresh, unstained human brain tissue could be clearly recognized. We further demonstrate THG images taken with a GRIN objective, as a step toward in situ THG microendoscopy of tumor boundaries. THG imaging is thus a promising tool for optical biopsies.
Original languageEnglish
Article number5
Pages (from-to)1889-1904
JournalBiomedical Optics Express
Issue number7
DOIs
Publication statusPublished - 2016

    Fingerprint

Cite this