Thirteen weeks of supplementation of vitamin D and leucine-enriched whey protein nutritional supplement attenuates chronic low-grade inflammation in sarcopenic older adults: the PROVIDE study

Keliane Liberman; Rose Njemini; Yvette Luiking; Louis N. Forti; Sjors Verlaan; Jürgen M. Bauer; Robert Memelink; Kirsten Brandt; Lorenzo M. Donini; Marcello Maggio; Tony Mets; Sander L. J. Wijers; Cornel Sieber; Tommy Cederholm; Ivan Bautmans

doi:10.1007/s40520-019-01208-4

Thirteen weeks of supplementation of vitamin D and leucine-enriched whey protein nutritional supplement attenuates chronic low-grade inflammation in sarcopenic older adults: the PROVIDE study

Keliane Liberman, Rose Njemini, Yvette Luiking, Louis N. Forti, Sjors Verlaan, Jürgen M. Bauer, Robert Memelink, Kirsten Brandt, Lorenzo M. Donini, Marcello Maggio, Tony Mets, Sander L. J. Wijers, Cornel Sieber, Tommy Cederholm, Ivan Bautmans

Physiology

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Background: A chronic low-grade inflammatory profile (CLIP) is associated with sarcopenia in older adults. Protein and Vitamin (Vit)D have immune-modulatory potential, but evidence for effects of nutritional supplementation on CLIP is limited. Aim: To investigate whether 13 weeks of nutritional supplementation of VitD and leucine-enriched whey protein affected CLIP in subjects enrolled in the PROVIDE-study, as a secondary analysis. Methods: Sarcopenic adults (low skeletal muscle mass) aged ≥ 65 years with mobility limitations (Short Physical Performance Battery 4–9) and a body mass index of 20–30 kg/m ² were randomly allocated to two daily servings of active (n = 137, including 20 g of whey protein, 3 g of leucine and 800 IU VitD) or isocaloric control product (n = 151) for a double-blind period of 13 weeks. At baseline and after 13 weeks, circulating interleukin (IL)-8, IL-1 receptor antagonist (RA), soluble tumor-necrosis-factor receptor (sTNFR)1, IL-6, high-sensitivity C-reactive protein, pre-albumin and 25-hydroxyvitamin(OH)D were measured. Data-analysis included repeated measures analysis of covariance (corrected for dietary VitD intake) and linear regression. Results: IL-6 and IL-1Ra serum levels showed overall increases after 13 weeks (p = 0.006 and p < 0.001, respectively). For IL-6 a significant time × treatment interaction (p = 0.046) was observed, with no significant change over time in the active group (p = 0.155) compared to control (significant increase p = 0.012). IL-8 showed an overall significant decrease (p = 0.03). The change in pre-albumin was a significant predictor for changes in IL-6 after 13 weeks. Conclusions: We conclude that 13 weeks of nutritional supplementation with VitD and leucine-enriched whey protein may attenuate the progression of CLIP in older sarcopenic persons with mobility limitations.

Original language	English
Pages (from-to)	845-854
Number of pages	10
Journal	Aging Clinical and Experimental Research
Volume	31
Issue number	6
DOIs	https://doi.org/10.1007/s40520-019-01208-4
Publication status	Published - 1 Jun 2019

Funding

We thank the participants for giving their time and energy to volunteer for this trial. We are grateful to the research staff members at each study site for their energy and commitment to achieving the aims of this study. We thank Dr. Wilfried Cools ("Interfaculty Center Data processing and Statistics (ICDS), Vrije Universiteit Brussel) for the bio-statistical advice. We are thankful to the PROVIDE study group* for their professional oversight and guidance. This study was financially supported and study products were provided by Nutricia Research, Nutricia Advanced Medical Nutrition. *PROVIDE study group members: Jürgen M. Bauer, Sjors Verlaan, Ivan Bautmans, Kirsten Brandt, Lorenzo M. Donini, Marcello Maggio, Marion E. T. McMurdo, Tony Mets, Chris Seal, Sander L. Wijers, Gian Paolo Ceda, Giuseppe De Vito, Gilbert Donders, Michael Drey, Carolyn Greig, Ulf Holmbäck, Marco Narici, Jamie McPhee, Eleonora Poggiogalle, Dermot Power, Aldo Scafoglieri, Ralf Schultz, Cornel C. Sieber, Tommy Cederholm. Acknowledgements We thank the participants for giving their time and energy to volunteer for this trial. We are grateful to the research staff members at each study site for their energy and commitment to achieving the aims of this study. We thank Dr. Wilfried Cools ("Interfaculty Center Data processing and Statistics (ICDS), Vrije Universiteit Brussel) for the bio-statistical advice. We are thankful to the PROVIDE study group* for their professional oversight and guidance. This study was financially supported and study products were provided by Nutricia Research, Nutricia Advanced Medical Nutrition. *PROVIDE study group members: Jürgen M. Bauer, Sjors Verlaan, Ivan Bautmans, Kirsten Brandt, Lorenzo M. Donini, Marcello Maggio, Marion E. T. McMurdo, Tony Mets, Chris Seal, Sander L. Wijers, Gian Paolo Ceda, Giuseppe De Vito, Gilbert Donders, Michael Drey, Carolyn Greig, Ulf Holmbäck, Marco Narici, Jamie McPhee, Eleonora Poggiogalle, Der-mot Power, Aldo Scafoglieri, Ralf Schultz, Cornel C. Sieber, Tommy Cederholm.

Funders	Funder number
Nutricia Advanced Medical Nutrition
Nutricia Research
Vrije Universiteit Brussel

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Liberman, K., Njemini, R., Luiking, Y., Forti, L. N., Verlaan, S., Bauer, J. M., Memelink, R., Brandt, K., Donini, L. M., Maggio, M., Mets, T., Wijers, S. L. J., Sieber, C., Cederholm, T., & Bautmans, I. (2019). Thirteen weeks of supplementation of vitamin D and leucine-enriched whey protein nutritional supplement attenuates chronic low-grade inflammation in sarcopenic older adults: the PROVIDE study. Aging Clinical and Experimental Research, 31(6), 845-854. https://doi.org/10.1007/s40520-019-01208-4

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title = "Thirteen weeks of supplementation of vitamin D and leucine-enriched whey protein nutritional supplement attenuates chronic low-grade inflammation in sarcopenic older adults: the PROVIDE study",

abstract = "Background: A chronic low-grade inflammatory profile (CLIP) is associated with sarcopenia in older adults. Protein and Vitamin (Vit)D have immune-modulatory potential, but evidence for effects of nutritional supplementation on CLIP is limited. Aim: To investigate whether 13 weeks of nutritional supplementation of VitD and leucine-enriched whey protein affected CLIP in subjects enrolled in the PROVIDE-study, as a secondary analysis. Methods: Sarcopenic adults (low skeletal muscle mass) aged ≥ 65 years with mobility limitations (Short Physical Performance Battery 4–9) and a body mass index of 20–30 kg/m 2 were randomly allocated to two daily servings of active (n = 137, including 20 g of whey protein, 3 g of leucine and 800 IU VitD) or isocaloric control product (n = 151) for a double-blind period of 13 weeks. At baseline and after 13 weeks, circulating interleukin (IL)-8, IL-1 receptor antagonist (RA), soluble tumor-necrosis-factor receptor (sTNFR)1, IL-6, high-sensitivity C-reactive protein, pre-albumin and 25-hydroxyvitamin(OH)D were measured. Data-analysis included repeated measures analysis of covariance (corrected for dietary VitD intake) and linear regression. Results: IL-6 and IL-1Ra serum levels showed overall increases after 13 weeks (p = 0.006 and p < 0.001, respectively). For IL-6 a significant time × treatment interaction (p = 0.046) was observed, with no significant change over time in the active group (p = 0.155) compared to control (significant increase p = 0.012). IL-8 showed an overall significant decrease (p = 0.03). The change in pre-albumin was a significant predictor for changes in IL-6 after 13 weeks. Conclusions: We conclude that 13 weeks of nutritional supplementation with VitD and leucine-enriched whey protein may attenuate the progression of CLIP in older sarcopenic persons with mobility limitations. ",

author = "Keliane Liberman and Rose Njemini and Yvette Luiking and Forti, {Louis N.} and Sjors Verlaan and Bauer, {J{\"u}rgen M.} and Robert Memelink and Kirsten Brandt and Donini, {Lorenzo M.} and Marcello Maggio and Tony Mets and Wijers, {Sander L. J.} and Cornel Sieber and Tommy Cederholm and Ivan Bautmans",

year = "2019",

month = jun,

day = "1",

doi = "10.1007/s40520-019-01208-4",

language = "English",

volume = "31",

pages = "845--854",

journal = "Aging Clinical and Experimental Research",

issn = "1720-8319",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "6",

}

Liberman, K, Njemini, R, Luiking, Y, Forti, LN, Verlaan, S, Bauer, JM, Memelink, R, Brandt, K, Donini, LM, Maggio, M, Mets, T, Wijers, SLJ, Sieber, C, Cederholm, T & Bautmans, I 2019, 'Thirteen weeks of supplementation of vitamin D and leucine-enriched whey protein nutritional supplement attenuates chronic low-grade inflammation in sarcopenic older adults: the PROVIDE study', Aging Clinical and Experimental Research, vol. 31, no. 6, pp. 845-854. https://doi.org/10.1007/s40520-019-01208-4

Thirteen weeks of supplementation of vitamin D and leucine-enriched whey protein nutritional supplement attenuates chronic low-grade inflammation in sarcopenic older adults: the PROVIDE study. / Liberman, Keliane; Njemini, Rose; Luiking, Yvette et al.
In: Aging Clinical and Experimental Research, Vol. 31, No. 6, 01.06.2019, p. 845-854.

Research output: Contribution to Journal › Article › Academic › peer-review

TY - JOUR

T1 - Thirteen weeks of supplementation of vitamin D and leucine-enriched whey protein nutritional supplement attenuates chronic low-grade inflammation in sarcopenic older adults: the PROVIDE study

AU - Liberman, Keliane

AU - Njemini, Rose

AU - Luiking, Yvette

AU - Forti, Louis N.

AU - Verlaan, Sjors

AU - Bauer, Jürgen M.

AU - Memelink, Robert

AU - Brandt, Kirsten

AU - Donini, Lorenzo M.

AU - Maggio, Marcello

AU - Mets, Tony

AU - Wijers, Sander L. J.

AU - Sieber, Cornel

AU - Cederholm, Tommy

AU - Bautmans, Ivan

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Background: A chronic low-grade inflammatory profile (CLIP) is associated with sarcopenia in older adults. Protein and Vitamin (Vit)D have immune-modulatory potential, but evidence for effects of nutritional supplementation on CLIP is limited. Aim: To investigate whether 13 weeks of nutritional supplementation of VitD and leucine-enriched whey protein affected CLIP in subjects enrolled in the PROVIDE-study, as a secondary analysis. Methods: Sarcopenic adults (low skeletal muscle mass) aged ≥ 65 years with mobility limitations (Short Physical Performance Battery 4–9) and a body mass index of 20–30 kg/m 2 were randomly allocated to two daily servings of active (n = 137, including 20 g of whey protein, 3 g of leucine and 800 IU VitD) or isocaloric control product (n = 151) for a double-blind period of 13 weeks. At baseline and after 13 weeks, circulating interleukin (IL)-8, IL-1 receptor antagonist (RA), soluble tumor-necrosis-factor receptor (sTNFR)1, IL-6, high-sensitivity C-reactive protein, pre-albumin and 25-hydroxyvitamin(OH)D were measured. Data-analysis included repeated measures analysis of covariance (corrected for dietary VitD intake) and linear regression. Results: IL-6 and IL-1Ra serum levels showed overall increases after 13 weeks (p = 0.006 and p < 0.001, respectively). For IL-6 a significant time × treatment interaction (p = 0.046) was observed, with no significant change over time in the active group (p = 0.155) compared to control (significant increase p = 0.012). IL-8 showed an overall significant decrease (p = 0.03). The change in pre-albumin was a significant predictor for changes in IL-6 after 13 weeks. Conclusions: We conclude that 13 weeks of nutritional supplementation with VitD and leucine-enriched whey protein may attenuate the progression of CLIP in older sarcopenic persons with mobility limitations.

AB - Background: A chronic low-grade inflammatory profile (CLIP) is associated with sarcopenia in older adults. Protein and Vitamin (Vit)D have immune-modulatory potential, but evidence for effects of nutritional supplementation on CLIP is limited. Aim: To investigate whether 13 weeks of nutritional supplementation of VitD and leucine-enriched whey protein affected CLIP in subjects enrolled in the PROVIDE-study, as a secondary analysis. Methods: Sarcopenic adults (low skeletal muscle mass) aged ≥ 65 years with mobility limitations (Short Physical Performance Battery 4–9) and a body mass index of 20–30 kg/m 2 were randomly allocated to two daily servings of active (n = 137, including 20 g of whey protein, 3 g of leucine and 800 IU VitD) or isocaloric control product (n = 151) for a double-blind period of 13 weeks. At baseline and after 13 weeks, circulating interleukin (IL)-8, IL-1 receptor antagonist (RA), soluble tumor-necrosis-factor receptor (sTNFR)1, IL-6, high-sensitivity C-reactive protein, pre-albumin and 25-hydroxyvitamin(OH)D were measured. Data-analysis included repeated measures analysis of covariance (corrected for dietary VitD intake) and linear regression. Results: IL-6 and IL-1Ra serum levels showed overall increases after 13 weeks (p = 0.006 and p < 0.001, respectively). For IL-6 a significant time × treatment interaction (p = 0.046) was observed, with no significant change over time in the active group (p = 0.155) compared to control (significant increase p = 0.012). IL-8 showed an overall significant decrease (p = 0.03). The change in pre-albumin was a significant predictor for changes in IL-6 after 13 weeks. Conclusions: We conclude that 13 weeks of nutritional supplementation with VitD and leucine-enriched whey protein may attenuate the progression of CLIP in older sarcopenic persons with mobility limitations.

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UR - http://www.scopus.com/inward/citedby.url?scp=85065308767&partnerID=8YFLogxK

U2 - 10.1007/s40520-019-01208-4

DO - 10.1007/s40520-019-01208-4

M3 - Article

SN - 1720-8319

VL - 31

SP - 845

EP - 854

JO - Aging Clinical and Experimental Research

JF - Aging Clinical and Experimental Research

IS - 6

ER -

Thirteen weeks of supplementation of vitamin D and leucine-enriched whey protein nutritional supplement attenuates chronic low-grade inflammation in sarcopenic older adults: the PROVIDE study

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