Three-dimensional molecular reconstruction of rat heart with mass spectrometry imaging

L. Fornai, A. Angelini, I. Klinkert, F. Giskes, A. Kiss, G. Eijkel, E.R. Amstalden-van Hove, L.A. Klerk, M. Fedrigo, G. Pieraccini, G. Moneti, M. Valente, G. Thiene, R.M.A Heeren

Research output: Contribution to JournalArticleAcademicpeer-review


Cardiovascular diseases are the world's number one cause of death, accounting for 17.1 million deaths a year. New high-resolution molecular and structural imaging strategies are needed to understand underlying pathophysiological mechanism. The aim of our study is (1) to provide a molecular basis of the heart animal model through the local identification of biomolecules by mass spectrometry imaging (MSI) (three-dimensional (3D) molecular reconstruction), (2) to perform a cross-species validation of secondary ion mass spectrometry (SIMS)- based cardiovascular molecular imaging, and (3) to demonstrate potential clinical relevance by the application of this innovative methodology to human heart specimens. We investigated a MSI approach using SIMS on the major areas of a rat and mouse heart: the pericardium, the myocardium, the endocardium, valves, and the great vessels. While several structures of the heart can be observed in individual two-dimensional sections analyzed by metal-assisted SIMS imaging, a full view of these structures in the total heart volume can be achieved only through the construction of the 3D heart model. The images of 3D reconstruction of the rat heart show a highly complementary localization between Na
Original languageEnglish
Pages (from-to)2927-2938
JournalAnalytical and Bioanalytical Chemistry
Issue number404
Publication statusPublished - 2012


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