Time dependent responses of glycolytic intermediates in a detailed glycolytic model of Lactococcus lactis during glucose run-out experiments.

M.H.N. Hoefnagel, A. van der Burgt, D.E. Martens, J. Hugenholtz, J.L. Snoep

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Glucose addition and subsequent run-out experiments were compared to simulations with a detailed glycolytic model of Lactococcus lactis. The model was constructed largely on bases of enzyme kinetic data taken from literature and not adjusted for the specific simulations shown here. Upon glucose depletion a rapid increase in PEP, inorganic phosphate and a gradual decrease in fructose 1,6-bisphosphate (FBP) were measured and predicted by simulation. The dynamic changes in these and other intermediate concentrations as measured in the experiments were well predicted by the kinetic model.
Original languageEnglish
Pages (from-to)157-162
JournalMolecular Biology Reports
Volume29
DOIs
Publication statusPublished - 2002

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