Time-resolved fluorescence of photosystem I in vivo: Global and target analysis

V. V. Chukhutsina; L. Tian; G. Ajlani; H. van Amerongen

doi:10.1007/978-3-642-32034-7_97

Time-resolved fluorescence of photosystem I in vivo: Global and target analysis

V. V. Chukhutsina, L. Tian, G. Ajlani, H. van Amerongen

Research output: Chapter in Book / Report / Conference proceeding › Chapter › Academic › peer-review

Abstract

Photosystem I (PSI) is a multi-pigment-protein complex that co-operates with photosystem II (PSII) and uses light energy to transfer electrons from plastocyanin or cytochrome to ferredoxin and eventually to NADP+. Cyanobacterial BE mutant cells that lack PSII and light-harvesting phycobilisomes, provided us with the possibility to study the picosecond fluorescence kinetics of PSI in vivo and the results were analyzed using global and target analysis. The obtained components (5.4 ± 0.14 ps spectral equilibration and 27.8 ± 0.09 ps excitation trapping) are very similar to those found before for isolated PSI. The rate of energy transfer from red-shifted chlorophyll a (Chl a) molecules to bulk Chls a occurs with a rate constant of (7.4–10.4 ps)⁻¹ whereas the reverse process occurs with a rate constant of (19.3–29.8 ps)⁻¹ according to the target analysis, whereas trapping from bulk Chls a occurs with rate (16.8–25.0 ps)⁻¹.

Original language	English
Title of host publication	Advanced Topics in Science and Technology in China
Publisher	Springer Science and Business Media Deutschland GmbH
Pages	465-468
Number of pages	4
DOIs	https://doi.org/10.1007/978-3-642-32034-7_97
Publication status	Published - 2013

Publication series

Name	Advanced Topics in Science and Technology in China
ISSN (Print)	1995-6819
ISSN (Electronic)	1995-6827

Bibliographical note

Funding Information:
We would like to thank Rob Koehorst and Arie van Hoek for initial help with the streak-camera measurements, Cor Wolfs and Sashka Krumova for initial help with the cell growth and Sergey Laptenok, Joris Snellenburg and Ivo van Stokkum for seminal contributions to the data analysis. This work was supported by HARVEST Marie Curie Research Training Network (PITN-GA-2009-238017) and the European Union Sixth Framework Programme grant MRTN-CT-2005-019481 to HvA and VVC.

Publisher Copyright:
© Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg 2013.

Funding

We would like to thank Rob Koehorst and Arie van Hoek for initial help with the streak-camera measurements, Cor Wolfs and Sashka Krumova for initial help with the cell growth and Sergey Laptenok, Joris Snellenburg and Ivo van Stokkum for seminal contributions to the data analysis. This work was supported by HARVEST Marie Curie Research Training Network (PITN-GA-2009-238017) and the European Union Sixth Framework Programme grant MRTN-CT-2005-019481 to HvA and VVC.

Keywords

BE mutant
Cyanobacteria
Streak camera
Target analysis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-3-642-32034-7_97

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Cite this

Chukhutsina, V. V., Tian, L., Ajlani, G., & van Amerongen, H. (2013). Time-resolved fluorescence of photosystem I in vivo: Global and target analysis. In Advanced Topics in Science and Technology in China (pp. 465-468). (Advanced Topics in Science and Technology in China). Springer Science and Business Media Deutschland GmbH. https://doi.org/10.1007/978-3-642-32034-7_97

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abstract = "Photosystem I (PSI) is a multi-pigment-protein complex that co-operates with photosystem II (PSII) and uses light energy to transfer electrons from plastocyanin or cytochrome to ferredoxin and eventually to NADP+. Cyanobacterial BE mutant cells that lack PSII and light-harvesting phycobilisomes, provided us with the possibility to study the picosecond fluorescence kinetics of PSI in vivo and the results were analyzed using global and target analysis. The obtained components (5.4 ± 0.14 ps spectral equilibration and 27.8 ± 0.09 ps excitation trapping) are very similar to those found before for isolated PSI. The rate of energy transfer from red-shifted chlorophyll a (Chl a) molecules to bulk Chls a occurs with a rate constant of (7.4–10.4 ps)−1 whereas the reverse process occurs with a rate constant of (19.3–29.8 ps)−1 according to the target analysis, whereas trapping from bulk Chls a occurs with rate (16.8–25.0 ps)−1.",

keywords = "BE mutant, Cyanobacteria, Streak camera, Target analysis",

author = "Chukhutsina, {V. V.} and L. Tian and G. Ajlani and {van Amerongen}, H.",

note = "Funding Information: We would like to thank Rob Koehorst and Arie van Hoek for initial help with the streak-camera measurements, Cor Wolfs and Sashka Krumova for initial help with the cell growth and Sergey Laptenok, Joris Snellenburg and Ivo van Stokkum for seminal contributions to the data analysis. This work was supported by HARVEST Marie Curie Research Training Network (PITN-GA-2009-238017) and the European Union Sixth Framework Programme grant MRTN-CT-2005-019481 to HvA and VVC. Publisher Copyright: {\textcopyright} Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg 2013.",

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Time-resolved fluorescence of photosystem I in vivo: Global and target analysis. / Chukhutsina, V. V.; Tian, L.; Ajlani, G. et al.
Advanced Topics in Science and Technology in China. Springer Science and Business Media Deutschland GmbH, 2013. p. 465-468 (Advanced Topics in Science and Technology in China).

Research output: Chapter in Book / Report / Conference proceeding › Chapter › Academic › peer-review

TY - CHAP

T1 - Time-resolved fluorescence of photosystem I in vivo

T2 - Global and target analysis

AU - Chukhutsina, V. V.

AU - Tian, L.

AU - Ajlani, G.

AU - van Amerongen, H.

N1 - Funding Information: We would like to thank Rob Koehorst and Arie van Hoek for initial help with the streak-camera measurements, Cor Wolfs and Sashka Krumova for initial help with the cell growth and Sergey Laptenok, Joris Snellenburg and Ivo van Stokkum for seminal contributions to the data analysis. This work was supported by HARVEST Marie Curie Research Training Network (PITN-GA-2009-238017) and the European Union Sixth Framework Programme grant MRTN-CT-2005-019481 to HvA and VVC. Publisher Copyright: © Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg 2013.

PY - 2013

Y1 - 2013

N2 - Photosystem I (PSI) is a multi-pigment-protein complex that co-operates with photosystem II (PSII) and uses light energy to transfer electrons from plastocyanin or cytochrome to ferredoxin and eventually to NADP+. Cyanobacterial BE mutant cells that lack PSII and light-harvesting phycobilisomes, provided us with the possibility to study the picosecond fluorescence kinetics of PSI in vivo and the results were analyzed using global and target analysis. The obtained components (5.4 ± 0.14 ps spectral equilibration and 27.8 ± 0.09 ps excitation trapping) are very similar to those found before for isolated PSI. The rate of energy transfer from red-shifted chlorophyll a (Chl a) molecules to bulk Chls a occurs with a rate constant of (7.4–10.4 ps)−1 whereas the reverse process occurs with a rate constant of (19.3–29.8 ps)−1 according to the target analysis, whereas trapping from bulk Chls a occurs with rate (16.8–25.0 ps)−1.

AB - Photosystem I (PSI) is a multi-pigment-protein complex that co-operates with photosystem II (PSII) and uses light energy to transfer electrons from plastocyanin or cytochrome to ferredoxin and eventually to NADP+. Cyanobacterial BE mutant cells that lack PSII and light-harvesting phycobilisomes, provided us with the possibility to study the picosecond fluorescence kinetics of PSI in vivo and the results were analyzed using global and target analysis. The obtained components (5.4 ± 0.14 ps spectral equilibration and 27.8 ± 0.09 ps excitation trapping) are very similar to those found before for isolated PSI. The rate of energy transfer from red-shifted chlorophyll a (Chl a) molecules to bulk Chls a occurs with a rate constant of (7.4–10.4 ps)−1 whereas the reverse process occurs with a rate constant of (19.3–29.8 ps)−1 according to the target analysis, whereas trapping from bulk Chls a occurs with rate (16.8–25.0 ps)−1.

KW - BE mutant

KW - Cyanobacteria

KW - Streak camera

KW - Target analysis

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M3 - Chapter

AN - SCOPUS:84994052273

T3 - Advanced Topics in Science and Technology in China

SP - 465

EP - 468

BT - Advanced Topics in Science and Technology in China

PB - Springer Science and Business Media Deutschland GmbH

ER -

Time-resolved fluorescence of photosystem I in vivo: Global and target analysis

Abstract

Publication series

Bibliographical note

Funding

Keywords

UN SDGs

Access to Document

Persistent URL (handle)

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Cite this