Timed GDNF gene therapy using an immune-evasive gene switch promotes long distance axon regeneration

Ruben Eggers*, Fred De Winter, Stefan A. Hoyng, Rob C. Hoeben, Martijn J.A. Malessy, Martijn R. Tannemaat, Joost Verhaagen

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Neurosurgical repair in patients with proximal nerve lesions results in unsatisfactory recovery of function. Gene therapy for neurotrophic factors is a powerful strategy to promote axon regeneration. Glial cell line-derived neurotrophic factor (GDNF) gene therapy promotes motor neuron survival and axon outgrowth; however, uncontrolled delivery of GDNF results in axon entrapment. We report that time-restricted GDNF expression (1 month) using an immune-evasive doxycycline-inducible gene switch attenuated local axon entrapment in avulsed reimplanted ventral spinal roots, was sufficient to promote long-term motor neuron survival (24 weeks) and facilitated the recovery of compound muscle action potentials by 8 weeks. These improvements were associated with an increase in long-distance regeneration of motor axons. In contrast, persistent GDNF expression impaired axon regeneration by inducing axon entrapment. These findings demonstrate that timed expression can resolve the deleterious effect of uncontrolled growth factor delivery and shows that inducible growth factor gene therapy can be employed to enhance the efficacy of axon regeneration after neurosurgical repair of a proximal nerve lesion in rats. This preclinical study is an important step in the ongoing development of a neurotrophic factor gene therapy for patients with severe proximal nerve lesions.

Original languageEnglish
Pages (from-to)295-311
Number of pages17
JournalBrain
Volume142
Issue number2
Early online date15 Jan 2019
DOIs
Publication statusPublished - Feb 2019

Keywords

  • chronic denervation
  • GDNF
  • gene therapy
  • long distance regeneration
  • ventral root avulsion

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    Eggers, R., De Winter, F., Hoyng, S. A., Hoeben, R. C., Malessy, M. J. A., Tannemaat, M. R., & Verhaagen, J. (2019). Timed GDNF gene therapy using an immune-evasive gene switch promotes long distance axon regeneration. Brain, 142(2), 295-311. https://doi.org/10.1093/brain/awy340