Abstract
The selection of specific binding molecules like peptides and proteins from biolibraries using, for instance, phage display methods can be quite time-consuming. It is therefore desirable to develop a strategy that is much faster in selection and sorting of potential binders out of a biolibrary. In this contribution we separately discuss the current achievements in generation of biolibraries, single-molecule detection techniques and microfluidic devices. A high-throughput microfluidic platform is then proposed that combines the propulsion of liquid containing fluorescent components of the biolibrary through microchannels, single-molecule fluorescence photon burst detection and real-time sorting of positive hits. © 2004 Bentham Science Publishers Ltd.
Original language | English |
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Pages (from-to) | 173-179 |
Number of pages | 7 |
Journal | Current Pharmaceutical Biotechnology |
Volume | 5 |
DOIs | |
Publication status | Published - 2004 |