Toxicity, bioaccumulation and biotransformation of Cu oxide nanoparticles in Daphnia magna

Joyce Ribeiro Santos-Rasera, Analder Sant'Anna Neto, Regina Teresa Rosim Monteiro, Cornelis A.M. Van Gestel, Hudson Wallace Pereira De Carvalho

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

This study investigated the toxicity, bioaccumulation and biotransformation of copper oxide nanoparticles (nCuO) and CuSO4 in Daphnia magna. We performed acute and chronic assays, and analyzed the organisms by μ-XRF and μ-XANES. In acute assays 25 nm nCuO (LC50 0.05 ± 0.011 mg Cu per L) and CuSO4 (LC50 0.16 ± 0.015 mg Cu per L) were most toxic, while 40 nm and 80 nm nCuO had similar toxicity (LC50 2.34 ± 0.479 and 2.26 ± 0.246 mg Cu per L, respectively). In chronic assays, CuSO4 (EC50 1.7 × 10-4 ± 1.0 × 10-4 mg Cu per L) was most toxic followed by 25 nm nCuO (EC50 1.8 × 10-3 ± 8.0 × 10-4 mg Cu per L), while 40 and 80 nm nCuO were least toxic (EC50 2.10 ± 0.669 and 1.95 ± 0.568 mg Cu per L, respectively). μ-XRF showed that Cu was accumulated in the intestine and appendages of the daphnids. μ-XANES showed that 25 nm nCuO and CuSO4 were biotransformed into Cu3(PO4)2 (acute assays), whereas 40 and 80 nm nCuO remained as CuO (chronic assays). The higher toxicity exhibited by CuSO4 and 25 nm nCuO can be explained from their higher chemical reactivity (probed by catalytic decomposition of H2O2 and μ-XANES) compared to 40 and 80 nm nCuO.

Original languageEnglish
Pages (from-to)2897-2906
Number of pages10
JournalEnvironmental Science: Nano
Volume6
Issue number9
Early online date5 Aug 2019
DOIs
Publication statusPublished - 1 Sep 2019

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Bioaccumulation
biotransformation
Oxides
bioaccumulation
Toxicity
Assays
oxide
assay
Nanoparticles
Poisons
toxicity
X-ray fluorescence
Chemical reactivity
Copper oxides
Biotransformation
nanoparticle
decomposition
copper
Decomposition

Cite this

Santos-Rasera, J. R., Sant'Anna Neto, A., Rosim Monteiro, R. T., Van Gestel, C. A. M., & Pereira De Carvalho, H. W. (2019). Toxicity, bioaccumulation and biotransformation of Cu oxide nanoparticles in Daphnia magna. Environmental Science: Nano, 6(9), 2897-2906. https://doi.org/10.1039/c9en00280d
Santos-Rasera, Joyce Ribeiro ; Sant'Anna Neto, Analder ; Rosim Monteiro, Regina Teresa ; Van Gestel, Cornelis A.M. ; Pereira De Carvalho, Hudson Wallace. / Toxicity, bioaccumulation and biotransformation of Cu oxide nanoparticles in Daphnia magna. In: Environmental Science: Nano. 2019 ; Vol. 6, No. 9. pp. 2897-2906.
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abstract = "This study investigated the toxicity, bioaccumulation and biotransformation of copper oxide nanoparticles (nCuO) and CuSO4 in Daphnia magna. We performed acute and chronic assays, and analyzed the organisms by μ-XRF and μ-XANES. In acute assays 25 nm nCuO (LC50 0.05 ± 0.011 mg Cu per L) and CuSO4 (LC50 0.16 ± 0.015 mg Cu per L) were most toxic, while 40 nm and 80 nm nCuO had similar toxicity (LC50 2.34 ± 0.479 and 2.26 ± 0.246 mg Cu per L, respectively). In chronic assays, CuSO4 (EC50 1.7 × 10-4 ± 1.0 × 10-4 mg Cu per L) was most toxic followed by 25 nm nCuO (EC50 1.8 × 10-3 ± 8.0 × 10-4 mg Cu per L), while 40 and 80 nm nCuO were least toxic (EC50 2.10 ± 0.669 and 1.95 ± 0.568 mg Cu per L, respectively). μ-XRF showed that Cu was accumulated in the intestine and appendages of the daphnids. μ-XANES showed that 25 nm nCuO and CuSO4 were biotransformed into Cu3(PO4)2 (acute assays), whereas 40 and 80 nm nCuO remained as CuO (chronic assays). The higher toxicity exhibited by CuSO4 and 25 nm nCuO can be explained from their higher chemical reactivity (probed by catalytic decomposition of H2O2 and μ-XANES) compared to 40 and 80 nm nCuO.",
author = "Santos-Rasera, {Joyce Ribeiro} and {Sant'Anna Neto}, Analder and {Rosim Monteiro}, {Regina Teresa} and {Van Gestel}, {Cornelis A.M.} and {Pereira De Carvalho}, {Hudson Wallace}",
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Santos-Rasera, JR, Sant'Anna Neto, A, Rosim Monteiro, RT, Van Gestel, CAM & Pereira De Carvalho, HW 2019, 'Toxicity, bioaccumulation and biotransformation of Cu oxide nanoparticles in Daphnia magna' Environmental Science: Nano, vol. 6, no. 9, pp. 2897-2906. https://doi.org/10.1039/c9en00280d

Toxicity, bioaccumulation and biotransformation of Cu oxide nanoparticles in Daphnia magna. / Santos-Rasera, Joyce Ribeiro; Sant'Anna Neto, Analder; Rosim Monteiro, Regina Teresa; Van Gestel, Cornelis A.M.; Pereira De Carvalho, Hudson Wallace.

In: Environmental Science: Nano, Vol. 6, No. 9, 01.09.2019, p. 2897-2906.

Research output: Contribution to JournalArticleAcademicpeer-review

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T1 - Toxicity, bioaccumulation and biotransformation of Cu oxide nanoparticles in Daphnia magna

AU - Santos-Rasera, Joyce Ribeiro

AU - Sant'Anna Neto, Analder

AU - Rosim Monteiro, Regina Teresa

AU - Van Gestel, Cornelis A.M.

AU - Pereira De Carvalho, Hudson Wallace

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N2 - This study investigated the toxicity, bioaccumulation and biotransformation of copper oxide nanoparticles (nCuO) and CuSO4 in Daphnia magna. We performed acute and chronic assays, and analyzed the organisms by μ-XRF and μ-XANES. In acute assays 25 nm nCuO (LC50 0.05 ± 0.011 mg Cu per L) and CuSO4 (LC50 0.16 ± 0.015 mg Cu per L) were most toxic, while 40 nm and 80 nm nCuO had similar toxicity (LC50 2.34 ± 0.479 and 2.26 ± 0.246 mg Cu per L, respectively). In chronic assays, CuSO4 (EC50 1.7 × 10-4 ± 1.0 × 10-4 mg Cu per L) was most toxic followed by 25 nm nCuO (EC50 1.8 × 10-3 ± 8.0 × 10-4 mg Cu per L), while 40 and 80 nm nCuO were least toxic (EC50 2.10 ± 0.669 and 1.95 ± 0.568 mg Cu per L, respectively). μ-XRF showed that Cu was accumulated in the intestine and appendages of the daphnids. μ-XANES showed that 25 nm nCuO and CuSO4 were biotransformed into Cu3(PO4)2 (acute assays), whereas 40 and 80 nm nCuO remained as CuO (chronic assays). The higher toxicity exhibited by CuSO4 and 25 nm nCuO can be explained from their higher chemical reactivity (probed by catalytic decomposition of H2O2 and μ-XANES) compared to 40 and 80 nm nCuO.

AB - This study investigated the toxicity, bioaccumulation and biotransformation of copper oxide nanoparticles (nCuO) and CuSO4 in Daphnia magna. We performed acute and chronic assays, and analyzed the organisms by μ-XRF and μ-XANES. In acute assays 25 nm nCuO (LC50 0.05 ± 0.011 mg Cu per L) and CuSO4 (LC50 0.16 ± 0.015 mg Cu per L) were most toxic, while 40 nm and 80 nm nCuO had similar toxicity (LC50 2.34 ± 0.479 and 2.26 ± 0.246 mg Cu per L, respectively). In chronic assays, CuSO4 (EC50 1.7 × 10-4 ± 1.0 × 10-4 mg Cu per L) was most toxic followed by 25 nm nCuO (EC50 1.8 × 10-3 ± 8.0 × 10-4 mg Cu per L), while 40 and 80 nm nCuO were least toxic (EC50 2.10 ± 0.669 and 1.95 ± 0.568 mg Cu per L, respectively). μ-XRF showed that Cu was accumulated in the intestine and appendages of the daphnids. μ-XANES showed that 25 nm nCuO and CuSO4 were biotransformed into Cu3(PO4)2 (acute assays), whereas 40 and 80 nm nCuO remained as CuO (chronic assays). The higher toxicity exhibited by CuSO4 and 25 nm nCuO can be explained from their higher chemical reactivity (probed by catalytic decomposition of H2O2 and μ-XANES) compared to 40 and 80 nm nCuO.

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