Abstract
This study investigated the toxicity, bioaccumulation and biotransformation of copper oxide nanoparticles (nCuO) and CuSO4 in Daphnia magna. We performed acute and chronic assays, and analyzed the organisms by μ-XRF and μ-XANES. In acute assays 25 nm nCuO (LC50 0.05 ± 0.011 mg Cu per L) and CuSO4 (LC50 0.16 ± 0.015 mg Cu per L) were most toxic, while 40 nm and 80 nm nCuO had similar toxicity (LC50 2.34 ± 0.479 and 2.26 ± 0.246 mg Cu per L, respectively). In chronic assays, CuSO4 (EC50 1.7 × 10-4 ± 1.0 × 10-4 mg Cu per L) was most toxic followed by 25 nm nCuO (EC50 1.8 × 10-3 ± 8.0 × 10-4 mg Cu per L), while 40 and 80 nm nCuO were least toxic (EC50 2.10 ± 0.669 and 1.95 ± 0.568 mg Cu per L, respectively). μ-XRF showed that Cu was accumulated in the intestine and appendages of the daphnids. μ-XANES showed that 25 nm nCuO and CuSO4 were biotransformed into Cu3(PO4)2 (acute assays), whereas 40 and 80 nm nCuO remained as CuO (chronic assays). The higher toxicity exhibited by CuSO4 and 25 nm nCuO can be explained from their higher chemical reactivity (probed by catalytic decomposition of H2O2 and μ-XANES) compared to 40 and 80 nm nCuO.
| Original language | English |
|---|---|
| Pages (from-to) | 2897-2906 |
| Number of pages | 10 |
| Journal | Environmental Science: Nano |
| Volume | 6 |
| Issue number | 9 |
| Early online date | 5 Aug 2019 |
| DOIs | |
| Publication status | Published - 1 Sep 2019 |
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Toxicity, bioaccumulation and biotransformation of Cu oxide nanoparticles in Daphnia magna. / Santos-Rasera, Joyce Ribeiro; Sant'Anna Neto, Analder; Rosim Monteiro, Regina Teresa; Van Gestel, Cornelis A.M.; Pereira De Carvalho, Hudson Wallace.
In: Environmental Science: Nano, Vol. 6, No. 9, 01.09.2019, p. 2897-2906.Research output: Contribution to Journal › Article › Academic › peer-review
TY - JOUR
T1 - Toxicity, bioaccumulation and biotransformation of Cu oxide nanoparticles in Daphnia magna
AU - Santos-Rasera, Joyce Ribeiro
AU - Sant'Anna Neto, Analder
AU - Rosim Monteiro, Regina Teresa
AU - Van Gestel, Cornelis A.M.
AU - Pereira De Carvalho, Hudson Wallace
PY - 2019/9/1
Y1 - 2019/9/1
N2 - This study investigated the toxicity, bioaccumulation and biotransformation of copper oxide nanoparticles (nCuO) and CuSO4 in Daphnia magna. We performed acute and chronic assays, and analyzed the organisms by μ-XRF and μ-XANES. In acute assays 25 nm nCuO (LC50 0.05 ± 0.011 mg Cu per L) and CuSO4 (LC50 0.16 ± 0.015 mg Cu per L) were most toxic, while 40 nm and 80 nm nCuO had similar toxicity (LC50 2.34 ± 0.479 and 2.26 ± 0.246 mg Cu per L, respectively). In chronic assays, CuSO4 (EC50 1.7 × 10-4 ± 1.0 × 10-4 mg Cu per L) was most toxic followed by 25 nm nCuO (EC50 1.8 × 10-3 ± 8.0 × 10-4 mg Cu per L), while 40 and 80 nm nCuO were least toxic (EC50 2.10 ± 0.669 and 1.95 ± 0.568 mg Cu per L, respectively). μ-XRF showed that Cu was accumulated in the intestine and appendages of the daphnids. μ-XANES showed that 25 nm nCuO and CuSO4 were biotransformed into Cu3(PO4)2 (acute assays), whereas 40 and 80 nm nCuO remained as CuO (chronic assays). The higher toxicity exhibited by CuSO4 and 25 nm nCuO can be explained from their higher chemical reactivity (probed by catalytic decomposition of H2O2 and μ-XANES) compared to 40 and 80 nm nCuO.
AB - This study investigated the toxicity, bioaccumulation and biotransformation of copper oxide nanoparticles (nCuO) and CuSO4 in Daphnia magna. We performed acute and chronic assays, and analyzed the organisms by μ-XRF and μ-XANES. In acute assays 25 nm nCuO (LC50 0.05 ± 0.011 mg Cu per L) and CuSO4 (LC50 0.16 ± 0.015 mg Cu per L) were most toxic, while 40 nm and 80 nm nCuO had similar toxicity (LC50 2.34 ± 0.479 and 2.26 ± 0.246 mg Cu per L, respectively). In chronic assays, CuSO4 (EC50 1.7 × 10-4 ± 1.0 × 10-4 mg Cu per L) was most toxic followed by 25 nm nCuO (EC50 1.8 × 10-3 ± 8.0 × 10-4 mg Cu per L), while 40 and 80 nm nCuO were least toxic (EC50 2.10 ± 0.669 and 1.95 ± 0.568 mg Cu per L, respectively). μ-XRF showed that Cu was accumulated in the intestine and appendages of the daphnids. μ-XANES showed that 25 nm nCuO and CuSO4 were biotransformed into Cu3(PO4)2 (acute assays), whereas 40 and 80 nm nCuO remained as CuO (chronic assays). The higher toxicity exhibited by CuSO4 and 25 nm nCuO can be explained from their higher chemical reactivity (probed by catalytic decomposition of H2O2 and μ-XANES) compared to 40 and 80 nm nCuO.
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U2 - 10.1039/c9en00280d
DO - 10.1039/c9en00280d
M3 - Article
VL - 6
SP - 2897
EP - 2906
JO - Environmental Science: Nano
JF - Environmental Science: Nano
SN - 2051-8161
IS - 9
ER -