Toxicokinetics of zinc-oxide nanoparticles and zinc ions in the earthworm Eisenia andrei

The toxicokinetics of zinc in the earthworm Eisenia andrei was investigated following exposure for 21 days to ionic zinc (ZnCl₂) or zinc oxide nanoparticles (ZnO-NPs) in Lufa 2.2 soil, followed by 21 days elimination in clean soil. Two concentrations were tested for both ZnCl₂ (250 and 500 μg Zn g⁻¹) and ZnO-NPs (500 and 1000 μg Zn g⁻¹), corresponding to EC₂₅ and EC₅₀ for effects on reproduction. Based on the measured internal Zn concentrations in the earthworms over time of exposure, the kinetics parameters k_a – assimilation rate constant (g_soil g⁻¹ _{body weight} day⁻¹) and k_e – elimination rate constant (day⁻¹) were estimated using a one-compartment model for either total Zn concentrations in the soil or porewater Zn concentrations. In the ZnCl₂ treatments, k_a was higher for total Zn concentrations in soil, whereas in the ZnO-NP treatments, k_a was higher for porewater Zn concentrations. The value of k_e did not differ between the two Zn forms (ZnCl₂ vs ZnO-NPs) for either EC₅₀ or EC₂₅ when related to total Zn concentrations in soil, but for EC₅₀, k_e related to porewater Zn concentrations was significantly higher for ZnCl₂ than for ZnO-NPs. It is concluded that differences in kinetic parameters between treatments were connected with exposure concentrations rather than with the form of Zn. Zinc was efficiently regulated by the earthworms in all treatments: a 2-fold increase in exposure concentration resulted in a less than 2-fold increase in internal concentration, and after transfer to uncontaminated soil the internal Zn concentrations in the earthworms returned to ca 111 μgg⁻¹ dw in all treatments.