Transcription Factor-Based Fate Specification and Forward Programming for Neural Regeneration

Lea Flitsch, Karen Laupman, Oliver Brüstle*

*Corresponding author for this work

Research output: Contribution to JournalReview articleAcademicpeer-review

Abstract

Traditionally, in vitro generation of donor cells for brain repair has been dominated by the application of extrinsic growth factors and morphogens. Recent advances in cell engineering strategies such as reprogramming of somatic cells into induced pluripotent stem cells and direct cell fate conversion have impressively demonstrated the feasibility to manipulate cell identities by the overexpression of cell fate-determining transcription factors. These strategies are now increasingly implemented for transcription factor-guided differentiation of neural precursors and forward programming of pluripotent stem cells toward specific neural subtypes. This review covers major achievements, pros and cons, as well as future prospects of transcription factor-based cell fate specification and the applicability of these approaches for the generation of donor cells for brain repair.
Original languageEnglish
Article number121
Pages (from-to)1/22
Number of pages22
JournalFrontiers in Cellular Neuroscience
Volume14
DOIs
Publication statusPublished - 20 May 2020

Funding

This work was supported by grants from the European Union’s FP7 Program (FP7-HEALTH-F4-2013-602278-Neurostemcellrepair) and Horizon 2020 Program (H2020-667301-COSYN), the German Federal Ministry of Education and Research (01EK1603A-Neuro2D3), the ERA-Net Neuron Program (01EW1812A-TREAT-SNGAP), and the National Institute of Neurological Disorders and Stroke of the National Institutes of Health, United States (R01NS100514). The content is solely the responsibility of the authors and does not necessarily represent the official views of these funding agencies.

FundersFunder number
European Union’s FP7
FP7-HEALTH-F4-2013-602278-Neurostemcellrepair
National Institutes of HealthR01NS100514
National Institute of Neurological Disorders and Stroke
Horizon 2020 Framework Programme667301, H2020-667301-COSYN
Bundesministerium für Bildung und Forschung01EK1603A-Neuro2D3, 01EW1812A-TREAT-SNGAP

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