Transcriptome analysis of the response to chronic constant hypoxia in zebrafish hearts

I.J. Marques, J.T. Leito, H.P. Spaink, J. Testerink, R.T. Jaspers, F. Witte, S. van den Berg, C.P. Bagowski

    Research output: Contribution to JournalArticleAcademicpeer-review

    Abstract

    Insufficient blood supply during acute infarction and chronic ischemia leads to tissue hypoxia which can significantly alter gene expression patterns in the heart. In contrast to most mammals, some teleost fishes are able to adapt to extremely low oxygen levels. We describe here that chronic constant hypoxia (CCH) leads to a smaller ventricular outflow tract, reduced lacunae within the central ventricular cavity and around the trabeculae and an increase in the number of cardiac myocyte nuclei per area in the hearts of two teleost species, zebrafish (Danio rerio) and cichlids (Haplochromis piceatus). In order to identify the molecular basis for the adaptations to CCH, we profiled the gene expression changes in the hearts of adult zebrafish. We have analyzed over 15,000 different transcripts and found 376 differentially regulated genes, of which 260 genes showed increased and 116 genes decreased expression levels. Two notch receptors (notch-2 and notch-3) as well as regulatory genes linked to cell proliferation were transcriptionally upregulated in hypoxic hearts. We observed a simultaneous increase in expression of IGF-2 and IGFbp1 and upregulation of several genes important for the protection against reactive oxygen species (ROS). We have identified here many novel genes involved in the response to CCH in the heart, which may have potential clinical implications in the future. © 2007 Springer-Verlag.
    Original languageEnglish
    Pages (from-to)77-92
    JournalJournal of Comparative Physiology B. Biochemical, Systemic, and Environmental Physiology
    Volume178
    Issue number1
    DOIs
    Publication statusPublished - 2008

    Fingerprint

    Gene Expression Profiling
    Zebrafish
    hypoxia
    transcriptomics
    Danio rerio
    Genes
    heart
    Gene expression
    gene
    gene expression
    teleost
    Gene Expression
    genes
    Notch Receptors
    Cichlids
    Insulin-Like Growth Factor II
    Mammals
    infarction
    cichlid
    Cell proliferation

    Cite this

    Marques, I.J. ; Leito, J.T. ; Spaink, H.P. ; Testerink, J. ; Jaspers, R.T. ; Witte, F. ; van den Berg, S. ; Bagowski, C.P. / Transcriptome analysis of the response to chronic constant hypoxia in zebrafish hearts. In: Journal of Comparative Physiology B. Biochemical, Systemic, and Environmental Physiology. 2008 ; Vol. 178, No. 1. pp. 77-92.
    @article{dd0ecd2342434e8687461ffda2943f1b,
    title = "Transcriptome analysis of the response to chronic constant hypoxia in zebrafish hearts",
    abstract = "Insufficient blood supply during acute infarction and chronic ischemia leads to tissue hypoxia which can significantly alter gene expression patterns in the heart. In contrast to most mammals, some teleost fishes are able to adapt to extremely low oxygen levels. We describe here that chronic constant hypoxia (CCH) leads to a smaller ventricular outflow tract, reduced lacunae within the central ventricular cavity and around the trabeculae and an increase in the number of cardiac myocyte nuclei per area in the hearts of two teleost species, zebrafish (Danio rerio) and cichlids (Haplochromis piceatus). In order to identify the molecular basis for the adaptations to CCH, we profiled the gene expression changes in the hearts of adult zebrafish. We have analyzed over 15,000 different transcripts and found 376 differentially regulated genes, of which 260 genes showed increased and 116 genes decreased expression levels. Two notch receptors (notch-2 and notch-3) as well as regulatory genes linked to cell proliferation were transcriptionally upregulated in hypoxic hearts. We observed a simultaneous increase in expression of IGF-2 and IGFbp1 and upregulation of several genes important for the protection against reactive oxygen species (ROS). We have identified here many novel genes involved in the response to CCH in the heart, which may have potential clinical implications in the future. {\circledC} 2007 Springer-Verlag.",
    author = "I.J. Marques and J.T. Leito and H.P. Spaink and J. Testerink and R.T. Jaspers and F. Witte and {van den Berg}, S. and C.P. Bagowski",
    year = "2008",
    doi = "10.1007/s00360-007-0201-4",
    language = "English",
    volume = "178",
    pages = "77--92",
    journal = "Journal of Comparative Physiology B. Biochemical, Systemic, and Environmental Physiology",
    issn = "0174-1578",
    publisher = "Springer Verlag",
    number = "1",

    }

    Transcriptome analysis of the response to chronic constant hypoxia in zebrafish hearts. / Marques, I.J.; Leito, J.T.; Spaink, H.P.; Testerink, J.; Jaspers, R.T.; Witte, F.; van den Berg, S.; Bagowski, C.P.

    In: Journal of Comparative Physiology B. Biochemical, Systemic, and Environmental Physiology, Vol. 178, No. 1, 2008, p. 77-92.

    Research output: Contribution to JournalArticleAcademicpeer-review

    TY - JOUR

    T1 - Transcriptome analysis of the response to chronic constant hypoxia in zebrafish hearts

    AU - Marques, I.J.

    AU - Leito, J.T.

    AU - Spaink, H.P.

    AU - Testerink, J.

    AU - Jaspers, R.T.

    AU - Witte, F.

    AU - van den Berg, S.

    AU - Bagowski, C.P.

    PY - 2008

    Y1 - 2008

    N2 - Insufficient blood supply during acute infarction and chronic ischemia leads to tissue hypoxia which can significantly alter gene expression patterns in the heart. In contrast to most mammals, some teleost fishes are able to adapt to extremely low oxygen levels. We describe here that chronic constant hypoxia (CCH) leads to a smaller ventricular outflow tract, reduced lacunae within the central ventricular cavity and around the trabeculae and an increase in the number of cardiac myocyte nuclei per area in the hearts of two teleost species, zebrafish (Danio rerio) and cichlids (Haplochromis piceatus). In order to identify the molecular basis for the adaptations to CCH, we profiled the gene expression changes in the hearts of adult zebrafish. We have analyzed over 15,000 different transcripts and found 376 differentially regulated genes, of which 260 genes showed increased and 116 genes decreased expression levels. Two notch receptors (notch-2 and notch-3) as well as regulatory genes linked to cell proliferation were transcriptionally upregulated in hypoxic hearts. We observed a simultaneous increase in expression of IGF-2 and IGFbp1 and upregulation of several genes important for the protection against reactive oxygen species (ROS). We have identified here many novel genes involved in the response to CCH in the heart, which may have potential clinical implications in the future. © 2007 Springer-Verlag.

    AB - Insufficient blood supply during acute infarction and chronic ischemia leads to tissue hypoxia which can significantly alter gene expression patterns in the heart. In contrast to most mammals, some teleost fishes are able to adapt to extremely low oxygen levels. We describe here that chronic constant hypoxia (CCH) leads to a smaller ventricular outflow tract, reduced lacunae within the central ventricular cavity and around the trabeculae and an increase in the number of cardiac myocyte nuclei per area in the hearts of two teleost species, zebrafish (Danio rerio) and cichlids (Haplochromis piceatus). In order to identify the molecular basis for the adaptations to CCH, we profiled the gene expression changes in the hearts of adult zebrafish. We have analyzed over 15,000 different transcripts and found 376 differentially regulated genes, of which 260 genes showed increased and 116 genes decreased expression levels. Two notch receptors (notch-2 and notch-3) as well as regulatory genes linked to cell proliferation were transcriptionally upregulated in hypoxic hearts. We observed a simultaneous increase in expression of IGF-2 and IGFbp1 and upregulation of several genes important for the protection against reactive oxygen species (ROS). We have identified here many novel genes involved in the response to CCH in the heart, which may have potential clinical implications in the future. © 2007 Springer-Verlag.

    U2 - 10.1007/s00360-007-0201-4

    DO - 10.1007/s00360-007-0201-4

    M3 - Article

    VL - 178

    SP - 77

    EP - 92

    JO - Journal of Comparative Physiology B. Biochemical, Systemic, and Environmental Physiology

    JF - Journal of Comparative Physiology B. Biochemical, Systemic, and Environmental Physiology

    SN - 0174-1578

    IS - 1

    ER -