Transgenes and protein localization: myths and legends

F.M. Quattrocchio, C.E. Spelt, R.E. Koes

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Fluorescent protein (FP) fusions are frequently used to localize and follow the movement of proteins in living cells. However, a consensus is missing about the experimental design and controls that guarantee the reliability of the results. Here, we discuss possible artifacts and try to navigate through the many methods, preferences, and assumptions that surround protein localization in plants that make it difficult to design a universal approach to achieve reliable results. © 2013 Elsevier Ltd.
Original languageEnglish
Pages (from-to)473-476
JournalTrends in Plant Science
Issue number18
DOIs
Publication statusPublished - 2013

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