Translocation of an Intracellular Protein via Peptide-Directed Ligation

Christiane Stiller, Dennis M Krüger, Nicolas Brauckhoff, Marcel Schmidt, Petra Janning, Hazem Salamon, Tom N Grossmann

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Ligand-directed reactions allow chemical transformations at very low reactant concentrations and can thus provide access to efficient approaches for the post-translational modification of proteins. The development of these proximity-induced reactions is hampered by the number of appropriate ligands and the lack of design principles. Addressing these limitations, we report a proximity-induced labeling system which applies a moderate affinity peptide ligand. The design process was structure-guided and supported by molecular dynamics simulations. We show that selective protein labeling can be performed inside living cells enabling the subcellular translocation of a protein via ligand-directed chemistry for the first time.

Original languageEnglish
Pages (from-to)504-509
Number of pages6
JournalAcs chemical biology
Volume12
Issue number2
DOIs
Publication statusPublished - 17 Feb 2017

Fingerprint

Protein Transport
Ligation
Ligands
Peptides
Labeling
Proteins
Molecular Dynamics Simulation
Post Translational Protein Processing
Molecular dynamics
Chemical reactions
Cells
Computer simulation

Keywords

  • HeLa Cells
  • Humans
  • Ligands
  • Molecular Dynamics Simulation
  • Peptides
  • Protein Transport
  • Proteins
  • Journal Article

Cite this

Stiller, C., Krüger, D. M., Brauckhoff, N., Schmidt, M., Janning, P., Salamon, H., & Grossmann, T. N. (2017). Translocation of an Intracellular Protein via Peptide-Directed Ligation. Acs chemical biology, 12(2), 504-509. https://doi.org/10.1021/acschembio.6b01013
Stiller, Christiane ; Krüger, Dennis M ; Brauckhoff, Nicolas ; Schmidt, Marcel ; Janning, Petra ; Salamon, Hazem ; Grossmann, Tom N. / Translocation of an Intracellular Protein via Peptide-Directed Ligation. In: Acs chemical biology. 2017 ; Vol. 12, No. 2. pp. 504-509.
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Stiller, C, Krüger, DM, Brauckhoff, N, Schmidt, M, Janning, P, Salamon, H & Grossmann, TN 2017, 'Translocation of an Intracellular Protein via Peptide-Directed Ligation' Acs chemical biology, vol. 12, no. 2, pp. 504-509. https://doi.org/10.1021/acschembio.6b01013

Translocation of an Intracellular Protein via Peptide-Directed Ligation. / Stiller, Christiane; Krüger, Dennis M; Brauckhoff, Nicolas; Schmidt, Marcel; Janning, Petra; Salamon, Hazem; Grossmann, Tom N.

In: Acs chemical biology, Vol. 12, No. 2, 17.02.2017, p. 504-509.

Research output: Contribution to JournalArticleAcademicpeer-review

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AU - Salamon, Hazem

AU - Grossmann, Tom N

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Stiller C, Krüger DM, Brauckhoff N, Schmidt M, Janning P, Salamon H et al. Translocation of an Intracellular Protein via Peptide-Directed Ligation. Acs chemical biology. 2017 Feb 17;12(2):504-509. https://doi.org/10.1021/acschembio.6b01013