Transmission electron microscopy of bone

V. Everts, A. Niehof, W. Tigchelaar-Gutter, W. Beertsen

Research output: Contribution to JournalArticleAcademicpeer-review


This chapter describes procedures to process mineralized tissues obtained from different sources for transmission electron microscopy (TEM). Methods for fixation, resin embedding, staining of semi-thin sections and ultrathin sections are presented. In addition, attention will be paid to processing of cultured bone explants for TEM analysis.
Original languageEnglish
Pages (from-to)351-363
JournalMethods in Molecular Biology
Publication statusPublished - 2012


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