Tuning the Music: Acoustic Force Spectroscopy (AFS) 2.0

D. Kamsma; R. Creyghton; G. Sitters; G.J.L. Wuite; E.J.G. Peterman

doi:10.1016/j.ymeth.2016.05.002

Tuning the Music: Acoustic Force Spectroscopy (AFS) 2.0

D. Kamsma, R. Creyghton, G. Sitters, G.J.L. Wuite, E.J.G. Peterman

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

AFS is a recently introduced high-throughput single-molecule technique that allows studying structural and mechanochemical properties of many biomolecules in parallel. To further improve the method, we developed a modelling tool to optimize the layer thicknesses, and a calibration method to experimentally validate the modelled force profiles. After optimization, we are able to apply 350 pN on 4.5 μm polystyrene beads, without the use of an amplifier, at the coverslip side of the AFS chip. Furthermore, we present the use of a transparent piezo to generate the acoustic force and we show that AFS can be combined with high-NA oil or water-immersion objectives. With this set of developments AFS will be applicable to a broad range of single-molecule experiments.

Original language	English
Pages (from-to)	26-33
Journal	Methods
Volume	105
DOIs	https://doi.org/10.1016/j.ymeth.2016.05.002
Publication status	Published - 2016

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title = "Tuning the Music: Acoustic Force Spectroscopy (AFS) 2.0",

abstract = "AFS is a recently introduced high-throughput single-molecule technique that allows studying structural and mechanochemical properties of many biomolecules in parallel. To further improve the method, we developed a modelling tool to optimize the layer thicknesses, and a calibration method to experimentally validate the modelled force profiles. After optimization, we are able to apply 350 pN on 4.5 μm polystyrene beads, without the use of an amplifier, at the coverslip side of the AFS chip. Furthermore, we present the use of a transparent piezo to generate the acoustic force and we show that AFS can be combined with high-NA oil or water-immersion objectives. With this set of developments AFS will be applicable to a broad range of single-molecule experiments.",

author = "D. Kamsma and R. Creyghton and G. Sitters and G.J.L. Wuite and E.J.G. Peterman",

year = "2016",

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AU - Kamsma, D.

AU - Creyghton, R.

AU - Sitters, G.

AU - Wuite, G.J.L.

AU - Peterman, E.J.G.

PY - 2016

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AB - AFS is a recently introduced high-throughput single-molecule technique that allows studying structural and mechanochemical properties of many biomolecules in parallel. To further improve the method, we developed a modelling tool to optimize the layer thicknesses, and a calibration method to experimentally validate the modelled force profiles. After optimization, we are able to apply 350 pN on 4.5 μm polystyrene beads, without the use of an amplifier, at the coverslip side of the AFS chip. Furthermore, we present the use of a transparent piezo to generate the acoustic force and we show that AFS can be combined with high-NA oil or water-immersion objectives. With this set of developments AFS will be applicable to a broad range of single-molecule experiments.

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DO - 10.1016/j.ymeth.2016.05.002

M3 - Article

SN - 1046-2023

VL - 105

SP - 26

EP - 33

JO - Methods

JF - Methods

ER -

Tuning the Music: Acoustic Force Spectroscopy (AFS) 2.0

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