Two component system that regulates methanol and formaldehyde oxidation in Paracoccus denitrificans.

N. Harms, W.N.M. Reijnders, S. Koning, R.J.M. van Spanning

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    Abstract

    A chromosomal region encoding a two-component regulatory system, FlhRS, has been isolated from Paracoccus denitrificans. FlhRS-deficient mutants were unable to grow on methanol, methylamine, or choline as the carbon and energy source. Expression of the gene encoding glutathione-dependent formaldehyde dehydrogenase (fhlA) was undetectable in the mutant, and expression of the S-formylglutathione hydrolase gene (fghA) was reduced in the mutant background. In addition, methanol dehydrogenase was immunologically undetectable in cell extracts of FhlRS mutants. These results indicate that the FlhRS sensor-regulator pair is involved in the regulation of formaldehyde, methanol, and methylamine oxidation. The effect that the FlhRS proteins exert on the regulation of C
    Original languageEnglish
    Pages (from-to)664-670
    Number of pages7
    JournalJournal of Bacteriology
    Volume183
    DOIs
    Publication statusPublished - 2001

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