Ubiquitous Structural Signaling in Bacterial Phytochromes

Alexander Björling; Oskar Berntsson; Heikki Takala; Kevin D. Gallagher; Hardik Patel; Emil Gustavsson; Rachael St. Peter; Phu Duong; Angela Nugent; Fan Zhang; Peter Berntsen; Roberto Appio; Ivan Rajkovic; Heli Lehtivuori; Matthijs R. Panman; Maria Hoernke; Stephan Niebling; Rajiv Harimoorthy; Tilman Lamparter; Emina A. Stojković; Janne A. Ihalainen; Sebastian Westenhoff

doi:10.1021/acs.jpclett.5b01629

Ubiquitous Structural Signaling in Bacterial Phytochromes

Alexander Björling, Oskar Berntsson, Heikki Takala, Kevin D. Gallagher, Hardik Patel, Emil Gustavsson, Rachael St. Peter, Phu Duong, Angela Nugent, Fan Zhang, Peter Berntsen, Roberto Appio, Ivan Rajkovic, Heli Lehtivuori, Matthijs R. Panman, Maria Hoernke, Stephan Niebling, Rajiv Harimoorthy, Tilman Lamparter, Emina A. StojkovićJanne A. Ihalainen^*, Sebastian Westenhoff

^*Corresponding author for this work

Biophysics Photosynthesis/Energy

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

The phytochrome family of light-switchable proteins has long been studied by biochemical, spectroscopic and crystallographic means, while a direct probe for global conformational signal propagation has been lacking. Using solution X-ray scattering, we find that the photosensory cores of several bacterial phytochromes undergo similar large-scale structural changes upon red-light excitation. The data establish that phytochromes with ordinary and inverted photocycles share a structural signaling mechanism and that a particular conserved histidine, previously proposed to be involved in signal propagation, in fact tunes photoresponse.

Original language	English
Pages (from-to)	3379-3383
Number of pages	5
Journal	Journal of Physical Chemistry Letters
Volume	6
Issue number	17
DOIs	https://doi.org/10.1021/acs.jpclett.5b01629
Publication status	Published - 3 Sept 2015

Keywords

phytochromes
protein dynamics
signal transduction
X-ray scattering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acs.jpclett.5b01629

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Björling, A., Berntsson, O., Takala, H., Gallagher, K. D., Patel, H., Gustavsson, E., St. Peter, R., Duong, P., Nugent, A., Zhang, F., Berntsen, P., Appio, R., Rajkovic, I., Lehtivuori, H., Panman, M. R., Hoernke, M., Niebling, S., Harimoorthy, R., Lamparter, T., ... Westenhoff, S. (2015). Ubiquitous Structural Signaling in Bacterial Phytochromes. Journal of Physical Chemistry Letters, 6(17), 3379-3383. https://doi.org/10.1021/acs.jpclett.5b01629

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title = "Ubiquitous Structural Signaling in Bacterial Phytochromes",

abstract = "The phytochrome family of light-switchable proteins has long been studied by biochemical, spectroscopic and crystallographic means, while a direct probe for global conformational signal propagation has been lacking. Using solution X-ray scattering, we find that the photosensory cores of several bacterial phytochromes undergo similar large-scale structural changes upon red-light excitation. The data establish that phytochromes with ordinary and inverted photocycles share a structural signaling mechanism and that a particular conserved histidine, previously proposed to be involved in signal propagation, in fact tunes photoresponse. ",

keywords = "phytochromes, protein dynamics, signal transduction, X-ray scattering",

author = "Alexander Bj{\"o}rling and Oskar Berntsson and Heikki Takala and Gallagher, \{Kevin D.\} and Hardik Patel and Emil Gustavsson and \{St. Peter\}, Rachael and Phu Duong and Angela Nugent and Fan Zhang and Peter Berntsen and Roberto Appio and Ivan Rajkovic and Heli Lehtivuori and Panman, \{Matthijs R.\} and Maria Hoernke and Stephan Niebling and Rajiv Harimoorthy and Tilman Lamparter and Stojkovi{\'c}, \{Emina A.\} and Ihalainen, \{Janne A.\} and Sebastian Westenhoff",

year = "2015",

month = sep,

day = "3",

doi = "10.1021/acs.jpclett.5b01629",

language = "English",

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Björling, A, Berntsson, O, Takala, H, Gallagher, KD, Patel, H, Gustavsson, E, St. Peter, R, Duong, P, Nugent, A, Zhang, F, Berntsen, P, Appio, R, Rajkovic, I, Lehtivuori, H, Panman, MR, Hoernke, M, Niebling, S, Harimoorthy, R, Lamparter, T, Stojković, EA, Ihalainen, JA & Westenhoff, S 2015, 'Ubiquitous Structural Signaling in Bacterial Phytochromes', Journal of Physical Chemistry Letters, vol. 6, no. 17, pp. 3379-3383. https://doi.org/10.1021/acs.jpclett.5b01629

TY - JOUR

T1 - Ubiquitous Structural Signaling in Bacterial Phytochromes

AU - Björling, Alexander

AU - Berntsson, Oskar

AU - Takala, Heikki

AU - Gallagher, Kevin D.

AU - Patel, Hardik

AU - Gustavsson, Emil

AU - St. Peter, Rachael

AU - Duong, Phu

AU - Nugent, Angela

AU - Zhang, Fan

AU - Berntsen, Peter

AU - Appio, Roberto

AU - Rajkovic, Ivan

AU - Lehtivuori, Heli

AU - Panman, Matthijs R.

AU - Hoernke, Maria

AU - Niebling, Stephan

AU - Harimoorthy, Rajiv

AU - Lamparter, Tilman

AU - Stojković, Emina A.

AU - Ihalainen, Janne A.

AU - Westenhoff, Sebastian

PY - 2015/9/3

Y1 - 2015/9/3

N2 - The phytochrome family of light-switchable proteins has long been studied by biochemical, spectroscopic and crystallographic means, while a direct probe for global conformational signal propagation has been lacking. Using solution X-ray scattering, we find that the photosensory cores of several bacterial phytochromes undergo similar large-scale structural changes upon red-light excitation. The data establish that phytochromes with ordinary and inverted photocycles share a structural signaling mechanism and that a particular conserved histidine, previously proposed to be involved in signal propagation, in fact tunes photoresponse.

AB - The phytochrome family of light-switchable proteins has long been studied by biochemical, spectroscopic and crystallographic means, while a direct probe for global conformational signal propagation has been lacking. Using solution X-ray scattering, we find that the photosensory cores of several bacterial phytochromes undergo similar large-scale structural changes upon red-light excitation. The data establish that phytochromes with ordinary and inverted photocycles share a structural signaling mechanism and that a particular conserved histidine, previously proposed to be involved in signal propagation, in fact tunes photoresponse.

KW - phytochromes

KW - protein dynamics

KW - signal transduction

KW - X-ray scattering

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DO - 10.1021/acs.jpclett.5b01629

M3 - Article

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VL - 6

SP - 3379

EP - 3383

JO - Journal of Physical Chemistry Letters

JF - Journal of Physical Chemistry Letters

IS - 17

ER -

Ubiquitous Structural Signaling in Bacterial Phytochromes

Abstract

Keywords

UN SDGs

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