Uncovering the hidden ground state of green fluorescent protein

J.T.M. Kennis; D.S. Larsen; I.H.M. van Stokkum; M. Vengris; J.J. van Thor; R. van Grondelle

doi:10.1073/pnas.0404262102

Uncovering the hidden ground state of green fluorescent protein

J.T.M. Kennis, D.S. Larsen, I.H.M. van Stokkum, M. Vengris, J.J. van Thor, R. van Grondelle

Biophysics Photosynthesis/Energy

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

The fluorescence properties of GFP are strongly influenced by the protonation states of its chromophore and nearby amino acid side chains. In the ground state, the GFP chromophore is neutral and absorbs in the near UV. Upon excitation, the chromophore is deprotonated, and the resulting anionic chromophore emits its green fluorescence. So far, only excited-state intermediates have been observed in the GFP photocycle. We have used ultrafast multipulse control spectroscopy to prepare and directly observe GFP's hidden anionic ground-state intermediates as an integral part of the photocycle. Combined with dispersed multichannel detection and advanced global analysis techniques, the existence of two distinct anionic ground-state intermediates, I

Original language	English
Pages (from-to)	17988-17993
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	101
Issue number	52
DOIs	https://doi.org/10.1073/pnas.0404262102
Publication status	Published - 28 Dec 2004

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title = "Uncovering the hidden ground state of green fluorescent protein",

abstract = "The fluorescence properties of GFP are strongly influenced by the protonation states of its chromophore and nearby amino acid side chains. In the ground state, the GFP chromophore is neutral and absorbs in the near UV. Upon excitation, the chromophore is deprotonated, and the resulting anionic chromophore emits its green fluorescence. So far, only excited-state intermediates have been observed in the GFP photocycle. We have used ultrafast multipulse control spectroscopy to prepare and directly observe GFP's hidden anionic ground-state intermediates as an integral part of the photocycle. Combined with dispersed multichannel detection and advanced global analysis techniques, the existence of two distinct anionic ground-state intermediates, I",

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T1 - Uncovering the hidden ground state of green fluorescent protein

AU - Kennis, J.T.M.

AU - Larsen, D.S.

AU - van Stokkum, I.H.M.

AU - Vengris, M.

AU - van Thor, J.J.

AU - van Grondelle, R.

PY - 2004/12/28

Y1 - 2004/12/28

N2 - The fluorescence properties of GFP are strongly influenced by the protonation states of its chromophore and nearby amino acid side chains. In the ground state, the GFP chromophore is neutral and absorbs in the near UV. Upon excitation, the chromophore is deprotonated, and the resulting anionic chromophore emits its green fluorescence. So far, only excited-state intermediates have been observed in the GFP photocycle. We have used ultrafast multipulse control spectroscopy to prepare and directly observe GFP's hidden anionic ground-state intermediates as an integral part of the photocycle. Combined with dispersed multichannel detection and advanced global analysis techniques, the existence of two distinct anionic ground-state intermediates, I

AB - The fluorescence properties of GFP are strongly influenced by the protonation states of its chromophore and nearby amino acid side chains. In the ground state, the GFP chromophore is neutral and absorbs in the near UV. Upon excitation, the chromophore is deprotonated, and the resulting anionic chromophore emits its green fluorescence. So far, only excited-state intermediates have been observed in the GFP photocycle. We have used ultrafast multipulse control spectroscopy to prepare and directly observe GFP's hidden anionic ground-state intermediates as an integral part of the photocycle. Combined with dispersed multichannel detection and advanced global analysis techniques, the existence of two distinct anionic ground-state intermediates, I

U2 - 10.1073/pnas.0404262102

DO - 10.1073/pnas.0404262102

M3 - Article

SN - 0027-8424

VL - 101

SP - 17988

EP - 17993

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 52

ER -

Uncovering the hidden ground state of green fluorescent protein

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