Understanding the changes in the circular dichroism of light harvesting complex II upon varying its pigment composition and organization

S. Georgakopoulou, G. van der Zwan, R. Bassi, R. van Grondelle, H. van Amerongen, R. Croce

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

In this work we modeled the circular dichroism (CD) spectrum of LHCII, the main light harvesting antenna of photosystem II of higher plants. Excitonic calculations are performed for a monomelic subunit, taken from the crystal structure of trimeric LHCII from spinach [Liu, Z. F., Yan, H. C., Wang, K. B., Kuang, T. Y., Zhang, J. P., Gui, L. L., An, X. M., and Chang, W. R. (2004) Nature 428, 287-292]. All of the major features of the CD spectrum above 450 nm are satisfactorily reproduced, and possible orientations of the Ch1 and carotenoid transition dipole moments are identified. The obtained modeling parameters are used to simulate the CD spectra of two complexes with altered pigment composition: a mutant lacking Chls a 611-612 and a complex lacking the carotenoid neoxanthin. By removing the relevant pigment(s) from the structure, we are able to reproduce their spectra, which implies that the alteration does not disturb the overall structure. The CD spectrum of trimeric LHCII shows a reversed relative intensity of the two negative bands around 470 and 490 nm as compared to monomelic LHCII. The simulations reproduce this reversal, indicating that it is mainly due to interactions between chromophores in different monomelic subunits, and the trimerization does not induce observable changes in the monomelic structure. Our simulated spectrum resembles one of two different trimeric CD spectra reported in literature. We argue that the differences in the experimental trimeric CD spectra are caused by changes in the strength of the monomer-monomer interactions due to the differences in detergents used for the purification of the complexes. © 2007 American Chemical Society.
Original languageEnglish
Pages (from-to)4745-4754
JournalBiochemistry
Volume46
Issue number16
DOIs
Publication statusPublished - 2007

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Understanding the changes in the circular dichroism of light harvesting complex II upon varying its pigment composition and organization

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