Unexpected link between lipooligosaccharide biosynthesis and surface protein release in Mycobacterium marinum.

A.D. van der Woude, D. Sarkar, A. Bhatt, M. Sparrius, S.A. Raadsen, L. Boon, J.J.G. Geurtsen, E.N.G. Houben, G.S. Besra, W. Bitter, A.M. van der Sar, S. Luirink

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

The mycobacterial cell envelope is characterized by the presence of a highly impermeable second membrane, which is composed of mycolic acids intercalated with different unusual free lipids, such as lipooligosaccharides (LOS). Transport across this cell envelope requires a dedicated secretion system for extracellular proteins, such as PE-PGRS proteins, which are specific mycobacterial proteins with polymorphic GC-rich sequence (PGRS). In this study, we set out to identify novel components involved in the secretion of PE-PGRS proteins by screening Mycobacterium marinum transposon mutants for secretion defects. Interestingly, most mutants were not affected in secretion but in the release of PE-PGRS proteins from the cell surface. These mutants had insertions in a gene cluster associated with LOS biosynthesis. Lipid analysis of these mutants revealed a role at different stages of LOS biosynthesis for 10 novel genes. Furthermore, we show that regulatory protein WhiB4 is involved in LOS biosynthesis. The absence of the most extended LOS molecule, i.e. LOS-IV, and a concomitant accumulation of LOS-III was already sufficient to reduce the release of PE-PGRS proteins from the mycobacterial cell surface. A similar effect was observed for major surface protein EspE. These results show that the attachment of surface proteins is strongly influenced by the glycolipid composition of the mycobacterial cell envelope. Finally, we tested the virulence of a LOS-IV-deficient mutant in our zebrafish embryo infection model. This mutant showed a marked increase in virulence as compared with the wild-type strain, suggesting that LOS-IV plays a role in the modulation of mycobacterial virulence. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.
LanguageEnglish
Pages20417-20429
JournalJournal of Biological Chemistry
Volume287
Issue number24
Early online date13 Apr 2012
DOIs
Publication statusPublished - 2012

Fingerprint

Mycobacterium marinum
Biosynthesis
Membrane Proteins
GC Rich Sequence
Virulence
Proteins
Genes
Mycolic Acids
Lipids
lipid-linked oligosaccharides
Glycolipids
Zebrafish
Multigene Family
Screening
Embryonic Structures
Modulation

Cite this

van der Woude, A.D. ; Sarkar, D. ; Bhatt, A. ; Sparrius, M. ; Raadsen, S.A. ; Boon, L. ; Geurtsen, J.J.G. ; Houben, E.N.G. ; Besra, G.S. ; Bitter, W. ; van der Sar, A.M. ; Luirink, S. / Unexpected link between lipooligosaccharide biosynthesis and surface protein release in Mycobacterium marinum. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 24. pp. 20417-20429.
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title = "Unexpected link between lipooligosaccharide biosynthesis and surface protein release in Mycobacterium marinum.",
abstract = "The mycobacterial cell envelope is characterized by the presence of a highly impermeable second membrane, which is composed of mycolic acids intercalated with different unusual free lipids, such as lipooligosaccharides (LOS). Transport across this cell envelope requires a dedicated secretion system for extracellular proteins, such as PE-PGRS proteins, which are specific mycobacterial proteins with polymorphic GC-rich sequence (PGRS). In this study, we set out to identify novel components involved in the secretion of PE-PGRS proteins by screening Mycobacterium marinum transposon mutants for secretion defects. Interestingly, most mutants were not affected in secretion but in the release of PE-PGRS proteins from the cell surface. These mutants had insertions in a gene cluster associated with LOS biosynthesis. Lipid analysis of these mutants revealed a role at different stages of LOS biosynthesis for 10 novel genes. Furthermore, we show that regulatory protein WhiB4 is involved in LOS biosynthesis. The absence of the most extended LOS molecule, i.e. LOS-IV, and a concomitant accumulation of LOS-III was already sufficient to reduce the release of PE-PGRS proteins from the mycobacterial cell surface. A similar effect was observed for major surface protein EspE. These results show that the attachment of surface proteins is strongly influenced by the glycolipid composition of the mycobacterial cell envelope. Finally, we tested the virulence of a LOS-IV-deficient mutant in our zebrafish embryo infection model. This mutant showed a marked increase in virulence as compared with the wild-type strain, suggesting that LOS-IV plays a role in the modulation of mycobacterial virulence. {\circledC} 2012 by The American Society for Biochemistry and Molecular Biology, Inc.",
author = "{van der Woude}, A.D. and D. Sarkar and A. Bhatt and M. Sparrius and S.A. Raadsen and L. Boon and J.J.G. Geurtsen and E.N.G. Houben and G.S. Besra and W. Bitter and {van der Sar}, A.M. and S. Luirink",
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van der Woude, AD, Sarkar, D, Bhatt, A, Sparrius, M, Raadsen, SA, Boon, L, Geurtsen, JJG, Houben, ENG, Besra, GS, Bitter, W, van der Sar, AM & Luirink, S 2012, 'Unexpected link between lipooligosaccharide biosynthesis and surface protein release in Mycobacterium marinum.', Journal of Biological Chemistry, vol. 287, no. 24, pp. 20417-20429. https://doi.org/10.1074/jbc.M111.336461

Unexpected link between lipooligosaccharide biosynthesis and surface protein release in Mycobacterium marinum. / van der Woude, A.D.; Sarkar, D.; Bhatt, A.; Sparrius, M.; Raadsen, S.A.; Boon, L.; Geurtsen, J.J.G.; Houben, E.N.G.; Besra, G.S.; Bitter, W.; van der Sar, A.M.; Luirink, S.

In: Journal of Biological Chemistry, Vol. 287, No. 24, 2012, p. 20417-20429.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - Unexpected link between lipooligosaccharide biosynthesis and surface protein release in Mycobacterium marinum.

AU - van der Woude, A.D.

AU - Sarkar, D.

AU - Bhatt, A.

AU - Sparrius, M.

AU - Raadsen, S.A.

AU - Boon, L.

AU - Geurtsen, J.J.G.

AU - Houben, E.N.G.

AU - Besra, G.S.

AU - Bitter, W.

AU - van der Sar, A.M.

AU - Luirink, S.

PY - 2012

Y1 - 2012

N2 - The mycobacterial cell envelope is characterized by the presence of a highly impermeable second membrane, which is composed of mycolic acids intercalated with different unusual free lipids, such as lipooligosaccharides (LOS). Transport across this cell envelope requires a dedicated secretion system for extracellular proteins, such as PE-PGRS proteins, which are specific mycobacterial proteins with polymorphic GC-rich sequence (PGRS). In this study, we set out to identify novel components involved in the secretion of PE-PGRS proteins by screening Mycobacterium marinum transposon mutants for secretion defects. Interestingly, most mutants were not affected in secretion but in the release of PE-PGRS proteins from the cell surface. These mutants had insertions in a gene cluster associated with LOS biosynthesis. Lipid analysis of these mutants revealed a role at different stages of LOS biosynthesis for 10 novel genes. Furthermore, we show that regulatory protein WhiB4 is involved in LOS biosynthesis. The absence of the most extended LOS molecule, i.e. LOS-IV, and a concomitant accumulation of LOS-III was already sufficient to reduce the release of PE-PGRS proteins from the mycobacterial cell surface. A similar effect was observed for major surface protein EspE. These results show that the attachment of surface proteins is strongly influenced by the glycolipid composition of the mycobacterial cell envelope. Finally, we tested the virulence of a LOS-IV-deficient mutant in our zebrafish embryo infection model. This mutant showed a marked increase in virulence as compared with the wild-type strain, suggesting that LOS-IV plays a role in the modulation of mycobacterial virulence. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

AB - The mycobacterial cell envelope is characterized by the presence of a highly impermeable second membrane, which is composed of mycolic acids intercalated with different unusual free lipids, such as lipooligosaccharides (LOS). Transport across this cell envelope requires a dedicated secretion system for extracellular proteins, such as PE-PGRS proteins, which are specific mycobacterial proteins with polymorphic GC-rich sequence (PGRS). In this study, we set out to identify novel components involved in the secretion of PE-PGRS proteins by screening Mycobacterium marinum transposon mutants for secretion defects. Interestingly, most mutants were not affected in secretion but in the release of PE-PGRS proteins from the cell surface. These mutants had insertions in a gene cluster associated with LOS biosynthesis. Lipid analysis of these mutants revealed a role at different stages of LOS biosynthesis for 10 novel genes. Furthermore, we show that regulatory protein WhiB4 is involved in LOS biosynthesis. The absence of the most extended LOS molecule, i.e. LOS-IV, and a concomitant accumulation of LOS-III was already sufficient to reduce the release of PE-PGRS proteins from the mycobacterial cell surface. A similar effect was observed for major surface protein EspE. These results show that the attachment of surface proteins is strongly influenced by the glycolipid composition of the mycobacterial cell envelope. Finally, we tested the virulence of a LOS-IV-deficient mutant in our zebrafish embryo infection model. This mutant showed a marked increase in virulence as compared with the wild-type strain, suggesting that LOS-IV plays a role in the modulation of mycobacterial virulence. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

U2 - 10.1074/jbc.M111.336461

DO - 10.1074/jbc.M111.336461

M3 - Article

VL - 287

SP - 20417

EP - 20429

JO - Journal of Biological Chemistry

T2 - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

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