Unfolding of the C-Terminal J alpha Helix in the LOV2 Photoreceptor Domain Observed by Time-Resolved Vibrational Spectroscopy

P.E. Konold, T. Mathes, J. Weissenborn, M.L. Groot, P. Hegemann, J.T.M. Kennis

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Light-triggered reactions of biological photoreceptors have gained immense attention for their role as molecular switches in their native organisms and for optogenetic application. The light, oxygen, and voltage 2 (LOV2) sensing domain of plant phototropin binds a C-terminal Jα helix that is docked on a β-sheet and unfolds upon light absorption by the flavin mononucleotide (FMN) chromophore. In this work, the signal transduction pathway of LOV2 from Avena sativa was investigated using time-resolved infrared spectroscopy from picoseconds to microseconds. In D
Original languageEnglish
Pages (from-to)3472-3476
JournalJournal of Physical Chemistry Letters
Issue number17
Publication statusPublished - 2016


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