Unraveling the biophysical properties of chromatin proteins and DNA using acoustic force spectroscopy

Szu Ning Lin, Liang Qin, Gijs J.L. Wuite*, Remus T. Dame

*Corresponding author for this work

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review

Abstract

Acoustic Force Spectroscopy (AFS) is a single-molecule micromanipulation technique that uses sound waves to exert force on surface-tethered DNA molecules in a microfluidic chamber. As large numbers of individual protein-DNA complexes are tracked in parallel, AFS provides insight into the individual properties of such complexes as well as their population averages. In this chapter, we describe in detail how to perform AFS experiments specifically on bare DNA, protein-DNA complexes, and how to extract their (effective) persistence length and contour length from force-extension relations.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Subtitle of host publicationBacterial Chromatin
PublisherHumana Press Inc
Pages301-316
Number of pages16
Volume1837
ISBN (Electronic)978-1-4939-8675-0
ISBN (Print)978-1-4939-8674-3
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1837
ISSN (Print)1064-3745

Keywords

  • Acoustic force spectroscopy
  • Bacterial chromatin protein
  • DNA-binding protein
  • Protein-DNA interaction
  • Single-molecule manipulation

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