Unraveling the biophysical properties of chromatin proteins and DNA using acoustic force spectroscopy

Szu Ning Lin, Liang Qin, Gijs J.L. Wuite*, Remus T. Dame

*Corresponding author for this work

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review


Acoustic Force Spectroscopy (AFS) is a single-molecule micromanipulation technique that uses sound waves to exert force on surface-tethered DNA molecules in a microfluidic chamber. As large numbers of individual protein-DNA complexes are tracked in parallel, AFS provides insight into the individual properties of such complexes as well as their population averages. In this chapter, we describe in detail how to perform AFS experiments specifically on bare DNA, protein-DNA complexes, and how to extract their (effective) persistence length and contour length from force-extension relations.

Original languageEnglish
Title of host publicationBacterial Chromatin
EditorsRemus T. Dame
PublisherHumana Press Inc
Number of pages16
ISBN (Electronic)9781493986750
ISBN (Print)9781493986743, 9781493993628
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


The authors thank Andreas Biebricher for the development of surface passivation methods, Sandrine D’Haene, Seyda Acar, and Ramon van der Valk for DNA substrate design and technical assistance, LUMICKS B.V. for assistance in developing the AFS system. This work was supported by grants from FOM (R.T.D. and G.J.L.W.), NWO-VICI (R.T.D. and G.J.L.W.), HFSP (R.T.D.) and China Scholarship Council No. 201506880001 (L.Q.).

FundersFunder number
China Scholarship Council201506880001


    • Acoustic force spectroscopy
    • Bacterial chromatin protein
    • DNA-binding protein
    • Protein-DNA interaction
    • Single-molecule manipulation


    Dive into the research topics of 'Unraveling the biophysical properties of chromatin proteins and DNA using acoustic force spectroscopy'. Together they form a unique fingerprint.

    Cite this