Unraveling the complexity of flux regulation; a new method demonstrated for nutrient starvation in Saccharomyces cerevisiae.

S. Rossell; C.C. van der Weijden; P.A. Lindenbergh; J.H. van Tuijl; C. Francke; B.M. Bakker; H.V. Westerhoff

doi:10.1073/pnas.0509831103

Unraveling the complexity of flux regulation; a new method demonstrated for nutrient starvation in Saccharomyces cerevisiae.

S. Rossell, C.C. van der Weijden, P.A. Lindenbergh, J.H. van Tuijl, C. Francke, B.M. Bakker, H.V. Westerhoff

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Abstract

An important question is to what estent metabolic fluxes are regulated by gene expression or by metabolic regulation. There are two distinct aspects to this question: (i) the local regulation of the fluxes through the individual steps in the pathway and (ii) the influence of such local regulation on the pathway's flux. We developed regulation analysis so as to address the former aspect for all steps in a pathway. We demonstrate the method for the issue of how Saccharomyces cerevisiae regulates the fluxes through its individual glycolytic and fermentative enzymes when confronted with nutrient starvation. Regulation was dissected quantitatively into (i) changes in maximum enzyme activity (V

Original language	English
Pages (from-to)	2166-2171
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	103
DOIs	https://doi.org/10.1073/pnas.0509831103
Publication status	Published - 2006

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Rossell, S., van der Weijden, C. C., Lindenbergh, P. A., van Tuijl, J. H., Francke, C., Bakker, B. M., & Westerhoff, H. V. (2006). Unraveling the complexity of flux regulation; a new method demonstrated for nutrient starvation in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences of the United States of America, 103, 2166-2171. https://doi.org/10.1073/pnas.0509831103

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title = "Unraveling the complexity of flux regulation; a new method demonstrated for nutrient starvation in Saccharomyces cerevisiae.",

abstract = "An important question is to what estent metabolic fluxes are regulated by gene expression or by metabolic regulation. There are two distinct aspects to this question: (i) the local regulation of the fluxes through the individual steps in the pathway and (ii) the influence of such local regulation on the pathway's flux. We developed regulation analysis so as to address the former aspect for all steps in a pathway. We demonstrate the method for the issue of how Saccharomyces cerevisiae regulates the fluxes through its individual glycolytic and fermentative enzymes when confronted with nutrient starvation. Regulation was dissected quantitatively into (i) changes in maximum enzyme activity (V",

author = "S. Rossell and {van der Weijden}, C.C. and P.A. Lindenbergh and {van Tuijl}, J.H. and C. Francke and B.M. Bakker and H.V. Westerhoff",

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doi = "10.1073/pnas.0509831103",

language = "English",

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Rossell, S, van der Weijden, CC, Lindenbergh, PA, van Tuijl, JH, Francke, C, Bakker, BM & Westerhoff, HV 2006, 'Unraveling the complexity of flux regulation; a new method demonstrated for nutrient starvation in Saccharomyces cerevisiae.', Proceedings of the National Academy of Sciences of the United States of America, vol. 103, pp. 2166-2171. https://doi.org/10.1073/pnas.0509831103

Unraveling the complexity of flux regulation; a new method demonstrated for nutrient starvation in Saccharomyces cerevisiae. / Rossell, S.; van der Weijden, C.C.; Lindenbergh, P.A. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, 2006, p. 2166-2171.

Research output: Contribution to Journal › Article › Academic › peer-review

TY - JOUR

T1 - Unraveling the complexity of flux regulation; a new method demonstrated for nutrient starvation in Saccharomyces cerevisiae.

AU - Rossell, S.

AU - van der Weijden, C.C.

AU - Lindenbergh, P.A.

AU - van Tuijl, J.H.

AU - Francke, C.

AU - Bakker, B.M.

AU - Westerhoff, H.V.

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AB - An important question is to what estent metabolic fluxes are regulated by gene expression or by metabolic regulation. There are two distinct aspects to this question: (i) the local regulation of the fluxes through the individual steps in the pathway and (ii) the influence of such local regulation on the pathway's flux. We developed regulation analysis so as to address the former aspect for all steps in a pathway. We demonstrate the method for the issue of how Saccharomyces cerevisiae regulates the fluxes through its individual glycolytic and fermentative enzymes when confronted with nutrient starvation. Regulation was dissected quantitatively into (i) changes in maximum enzyme activity (V

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DO - 10.1073/pnas.0509831103

M3 - Article

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

ER -

Unraveling the complexity of flux regulation; a new method demonstrated for nutrient starvation in Saccharomyces cerevisiae.

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