TY - JOUR
T1 - Unraveling the complexity of flux regulation; a new method demonstrated for nutrient starvation in Saccharomyces cerevisiae.
AU - Rossell, S.
AU - van der Weijden, C.C.
AU - Lindenbergh, P.A.
AU - van Tuijl, J.H.
AU - Francke, C.
AU - Bakker, B.M.
AU - Westerhoff, H.V.
PY - 2006
Y1 - 2006
N2 - An important question is to what estent metabolic fluxes are regulated by gene expression or by metabolic regulation. There are two distinct aspects to this question: (i) the local regulation of the fluxes through the individual steps in the pathway and (ii) the influence of such local regulation on the pathway's flux. We developed regulation analysis so as to address the former aspect for all steps in a pathway. We demonstrate the method for the issue of how Saccharomyces cerevisiae regulates the fluxes through its individual glycolytic and fermentative enzymes when confronted with nutrient starvation. Regulation was dissected quantitatively into (i) changes in maximum enzyme activity (V
AB - An important question is to what estent metabolic fluxes are regulated by gene expression or by metabolic regulation. There are two distinct aspects to this question: (i) the local regulation of the fluxes through the individual steps in the pathway and (ii) the influence of such local regulation on the pathway's flux. We developed regulation analysis so as to address the former aspect for all steps in a pathway. We demonstrate the method for the issue of how Saccharomyces cerevisiae regulates the fluxes through its individual glycolytic and fermentative enzymes when confronted with nutrient starvation. Regulation was dissected quantitatively into (i) changes in maximum enzyme activity (V
UR - https://www.scopus.com/pages/publications/33144474263
UR - https://www.scopus.com/inward/citedby.url?scp=33144474263&partnerID=8YFLogxK
U2 - 10.1073/pnas.0509831103
DO - 10.1073/pnas.0509831103
M3 - Article
SN - 0027-8424
VL - 103
SP - 2166
EP - 2171
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
ER -