Unraveling the Excited-State Dynamics and Light-Harvesting Functions of Xanthophylls in Light-Harvesting Complex II Using Femtosecond Stimulated Raman Spectroscopy

Juan M. Artes Vivancos, Ivo H.M. van Stokkum, Francesco Saccon, Yusaku Hontani, Miroslav Kloz, Alexander Ruban, Rienk van Grondelle, John T.M. Kennis*

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Photosynthesis in plants starts with the capture of photons by light-harvesting complexes (LHCs). Structural biology and spectroscopy approaches have led to a map of the architecture and energy transfer pathways between LHC pigments. Still, controversies remain regarding the role of specific carotenoids in light-harvesting and photoprotection, obligating the need for high-resolution techniques capable of identifying excited-state signatures and molecular identities of the various pigments in photosynthetic systems. Here we demonstrate the successful application of femtosecond stimulated Raman spectroscopy (FSRS) to a multichromophoric biological complex, trimers of LHCII. We demonstrate the application of global and target analysis (GTA) to FSRS data and utilize it to quantify excitation migration in LHCII trimers. This powerful combination of techniques allows us to obtain valuable insights into structural, electronic, and dynamic information from the carotenoids of LHCII trimers. We report spectral and dynamical information on ground- and excited-state vibrational modes of the different pigments, resolving the vibrational relaxation of the carotenoids and the pathways of energy transfer to chlorophylls. The lifetimes and spectral characteristics obtained for the S1 state confirm that lutein 2 has a distorted conformation in LHCII and that the lutein 2 S1 state does not transfer to chlorophylls, while lutein 1 is the only carotenoid whose S1 state plays a significant energy-harvesting role. No appreciable energy transfer takes place from lutein 1 to lutein 2, contradicting recent proposals regarding the functions of the various carotenoids (Son et al. Chem.2019, 5 (3), 575-584). Also, our results demonstrate that FSRS can be used in combination with GTA to simultaneously study the electronic and vibrational landscapes in LHCs and pave the way for in-depth studies of photoprotective conformations in photosynthetic systems.

Original languageEnglish
Pages (from-to)17346-17355
Number of pages10
JournalJournal of the American Chemical Society
Volume142
Issue number41
Early online date3 Sept 2020
DOIs
Publication statusPublished - 14 Oct 2020

Funding

This project has received funding from the European Union’s Horizon2020 program under the Marie Skłodowska-Curie grant agreements no. 660521 to J.M.A.V. and no. 675006 to FS. The NWO supported Y.H. and J.T.M.K. through a VICI grant, and a Middelgroot investment grant to J.T.M.K. A.V.R. acknowledges the support from the Royal Society Wolfson Research Merit Award WRMA2015/R1. M.K. was supported by Grant Agency of the Czech Republic project number 17-01137S. RvG was supported by an Advanced Investigator Grant from the European Research Council (no. 267333, PHOTPROT), the TOP-grant (700.58.305) from the Foundation of Chemical Science part of NWO, and the Canadian Institute for Advanced Research (CIFAR). R.v.G. gratefully acknowledges his Academy Professor grant from The Netherlands Royal Academy of Sciences (KNAW).

FundersFunder number
European Union’s Horizon2020
Foundation of Chemical Science
Koninklijke Nederlandse Akademie van Wetenschappen
Canadian Institute for Advanced Research
Horizon 2020 Framework Programme660521, 675006
Royal SocietyWRMA2015/R1
European Research Council267333, 700.58.305
Koninklijke Nederlandse Akademie van Wetenschappen
Grantová Agentura České Republiky17-01137S
Nederlandse Organisatie voor Wetenschappelijk Onderzoek

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