Unravelling the Biophysical Properties of Chromatin Proteins and DNA Using Acoustic Force Spectroscopy

Szu Ning Lin; Liang Qin; Kees Karel H. Taris; Gijs J.L. Wuite; Remus T. Dame

doi:10.1007/978-1-0716-3930-6_24

Unravelling the Biophysical Properties of Chromatin Proteins and DNA Using Acoustic Force Spectroscopy

Szu Ning Lin, Liang Qin, Kees Karel H. Taris, Gijs J.L. Wuite, Remus T. Dame

Research output: Chapter in Book / Report / Conference proceeding › Chapter › Academic › peer-review

Abstract

Acoustic force spectroscopy (AFS) is a single-molecule micromanipulation technique that uses sound waves to exert force on surface-tethered DNA molecules in a microfluidic chamber. As large numbers of individual protein-DNA complexes are tracked in parallel, AFS provides insight into the individual properties of such complexes as well as their population averages. In this chapter, we describe in detail how to perform AFS experiments specifically on bare DNA, protein-DNA complexes, and how to extract their (effective) persistence length and contour length from force-extension relations.

Original language	English
Title of host publication	Bacterial Chromatin
Subtitle of host publication	Methods and Protocols
Editors	Remus T. Dame
Publisher	Humana Press
Chapter	24
Pages	519-534
Number of pages	16
Edition	2nd
ISBN (Electronic)	9781071639306
ISBN (Print)	9781071639290 , 9781071639320
DOIs	https://doi.org/10.1007/978-1-0716-3930-6_24
Publication status	Published - 2024

Publication series

Name	Methods in molecular biology (Clifton, N.J.)
Publisher	Humana Press
Volume	2819
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Bacterial chromatin protein
DNA-binding protein
Protein–DNA interaction
Single-molecule manipulation
Acoustic force spectroscopy

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10.1007/978-1-0716-3930-6_24

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Lin, S. N., Qin, L., Taris, K. K. H., Wuite, G. J. L., & Dame, R. T. (2024). Unravelling the Biophysical Properties of Chromatin Proteins and DNA Using Acoustic Force Spectroscopy. In R. T. Dame (Ed.), Bacterial Chromatin: Methods and Protocols (2nd ed., pp. 519-534). (Methods in molecular biology (Clifton, N.J.); Vol. 2819). Humana Press. https://doi.org/10.1007/978-1-0716-3930-6_24

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Unravelling the Biophysical Properties of Chromatin Proteins and DNA Using Acoustic Force Spectroscopy. / Lin, Szu Ning; Qin, Liang; Taris, Kees Karel H. et al.
Bacterial Chromatin: Methods and Protocols. ed. / Remus T. Dame. 2nd. ed. Humana Press, 2024. p. 519-534 (Methods in molecular biology (Clifton, N.J.); Vol. 2819).

Research output: Chapter in Book / Report / Conference proceeding › Chapter › Academic › peer-review

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Unravelling the Biophysical Properties of Chromatin Proteins and DNA Using Acoustic Force Spectroscopy

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