Use of Reduced Gravity Simulators for Plant Biological Studies

Raúl Herranz; Miguel A. Valbuena; Aránzazu Manzano; Khaled Y. Kamal; Alicia Villacampa; Malgorzata Ciska; Jack J.W.A. van Loon; F. Javier Medina

doi:10.1007/978-1-0716-1677-2_16

Use of Reduced Gravity Simulators for Plant Biological Studies

Raúl Herranz^*, Miguel A. Valbuena, Aránzazu Manzano, Khaled Y. Kamal, Alicia Villacampa, Malgorzata Ciska, Jack J.W.A. van Loon, F. Javier Medina

^*Corresponding author for this work

Maxillofacial Surgery (AMC + VUmc)

Research output: Chapter in Book / Report / Conference proceeding › Chapter › Academic › peer-review

Abstract

Simulated microgravity and partial gravity research on Earth is a necessary complement to space research in real microgravity due to limitations of access to spaceflight. However, the use of ground-based facilities for reduced gravity simulation is far from simple. Microgravity simulation usually results in the need to consider secondary effects that appear in the generation of altered gravity. These secondary effects may interfere with gravity alteration in the changes observed in the biological processes under study. In addition to microgravity simulation, ground-based facilities are also capable of generating hypergravity or fractional gravity conditions whose effects on biological systems are worth being tested and compared with the results of microgravity exposure. Multiple technologies (2D clinorotation, random positioning machines, magnetic levitators, or centrifuges) and experimental hardware (different containers and substrates for seedlings or cell cultures) are available for these studies. Experimental requirements should be collectively and carefully considered in defining the optimal experimental design, taking into account that some environmental parameters, or life-support conditions, could be difficult to be provided in certain facilities. Using simulation facilities will allow us to anticipate, modify, or redefine the findings provided by the scarce available spaceflight opportunities.

Original language	English
Title of host publication	Plant Gravitropism
Subtitle of host publication	Methods and Protocols
Editors	Elison B. Blancaflor
Publisher	Humana Press Inc
Pages	241-265
Number of pages	25
Edition	2nd
ISBN (Electronic)	9781071616772
ISBN (Print)	9781071616765, 9781071616796
DOIs	https://doi.org/10.1007/978-1-0716-1677-2_16
Publication status	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2368
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Funding Information:
Most of the results and comments included in this book chapter have been the consequence of the authors? participation in several different ?CORA-ESA Access to GBF? Projects of the European Space Agency, allowing the utilization of European facilities for altered gravity simulation, in close collaboration with the respective GBF managers. One of the co-authors of this paper (JvL) is the manager of the facilities hosted at DESC at ESA-ESTEC (Noord-wijk, NL). Other facilities used are managed, respectively, by Dr. Hemmersbach (DLR), Dr. Pereda-Loth (Toulouse University), Dr. Hill (Nottingham University), and Dr. Christianen (Nijmegen University). We want to acknowledge Julio Martin Santos (3DOHMS) support in dedicated hardware design (3D printing and electronic components) to be used in our lab clinostats (obtained by UNZIP project grants from UNOOSA). Work performed in the authors? laboratory was financially supported by the Spanish Plan Estatal de Investigaci?n Cient?fica y Desarrollo Tec-nol?gico, Grants #ESP2015-64323-R and #RTI2018-099309-B-I00 (co-funded by EU-ERDF) to F.J.M. and a grant from European Space Agency contract# 4000107455/12/NL/PA awarded to J.J.W.A.v.L.

Publisher Copyright:
© 2022, Springer Science+Business Media, LLC, part of Springer Nature.

Funding

Most of the results and comments included in this book chapter have been the consequence of the authors? participation in several different ?CORA-ESA Access to GBF? Projects of the European Space Agency, allowing the utilization of European facilities for altered gravity simulation, in close collaboration with the respective GBF managers. One of the co-authors of this paper (JvL) is the manager of the facilities hosted at DESC at ESA-ESTEC (Noord-wijk, NL). Other facilities used are managed, respectively, by Dr. Hemmersbach (DLR), Dr. Pereda-Loth (Toulouse University), Dr. Hill (Nottingham University), and Dr. Christianen (Nijmegen University). We want to acknowledge Julio Martin Santos (3DOHMS) support in dedicated hardware design (3D printing and electronic components) to be used in our lab clinostats (obtained by UNZIP project grants from UNOOSA). Work performed in the authors? laboratory was financially supported by the Spanish Plan Estatal de Investigaci?n Cient?fica y Desarrollo Tec-nol?gico, Grants #ESP2015-64323-R and #RTI2018-099309-B-I00 (co-funded by EU-ERDF) to F.J.M. and a grant from European Space Agency contract# 4000107455/12/NL/PA awarded to J.J.W.A.v.L.

Funders	Funder number
Nijmegen University
Spanish Plan Estatal de Investigación Científica y Desarrollo Tec-nológico	2018-099309-B-I00, 2015-64323-R
UNOOSA
UNZIP
University of Nottingham
European Space Agency
Université de Toulouse
European Regional Development Fund	4000107455/12/NL/PA

Keywords

Cell suspension cultures
Clinostat
Large Diameter Centrifuge (LDC)
Magnetic levitation
Random positioning machine (RPM)
Seedlings

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10.1007/978-1-0716-1677-2_16

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Herranz, R., Valbuena, M. A., Manzano, A., Kamal, K. Y., Villacampa, A., Ciska, M., van Loon, J. J. W. A., & Medina, F. J. (2022). Use of Reduced Gravity Simulators for Plant Biological Studies. In E. B. Blancaflor (Ed.), Plant Gravitropism: Methods and Protocols (2nd ed., pp. 241-265). (Methods in Molecular Biology; Vol. 2368). Humana Press Inc. https://doi.org/10.1007/978-1-0716-1677-2_16

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abstract = "Simulated microgravity and partial gravity research on Earth is a necessary complement to space research in real microgravity due to limitations of access to spaceflight. However, the use of ground-based facilities for reduced gravity simulation is far from simple. Microgravity simulation usually results in the need to consider secondary effects that appear in the generation of altered gravity. These secondary effects may interfere with gravity alteration in the changes observed in the biological processes under study. In addition to microgravity simulation, ground-based facilities are also capable of generating hypergravity or fractional gravity conditions whose effects on biological systems are worth being tested and compared with the results of microgravity exposure. Multiple technologies (2D clinorotation, random positioning machines, magnetic levitators, or centrifuges) and experimental hardware (different containers and substrates for seedlings or cell cultures) are available for these studies. Experimental requirements should be collectively and carefully considered in defining the optimal experimental design, taking into account that some environmental parameters, or life-support conditions, could be difficult to be provided in certain facilities. Using simulation facilities will allow us to anticipate, modify, or redefine the findings provided by the scarce available spaceflight opportunities.",

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note = "Funding Information: Most of the results and comments included in this book chapter have been the consequence of the authors? participation in several different ?CORA-ESA Access to GBF? Projects of the European Space Agency, allowing the utilization of European facilities for altered gravity simulation, in close collaboration with the respective GBF managers. One of the co-authors of this paper (JvL) is the manager of the facilities hosted at DESC at ESA-ESTEC (Noord-wijk, NL). Other facilities used are managed, respectively, by Dr. Hemmersbach (DLR), Dr. Pereda-Loth (Toulouse University), Dr. Hill (Nottingham University), and Dr. Christianen (Nijmegen University). We want to acknowledge Julio Martin Santos (3DOHMS) support in dedicated hardware design (3D printing and electronic components) to be used in our lab clinostats (obtained by UNZIP project grants from UNOOSA). Work performed in the authors? laboratory was financially supported by the Spanish Plan Estatal de Investigaci?n Cient?fica y Desarrollo Tec-nol?gico, Grants #ESP2015-64323-R and #RTI2018-099309-B-I00 (co-funded by EU-ERDF) to F.J.M. and a grant from European Space Agency contract# 4000107455/12/NL/PA awarded to J.J.W.A.v.L. Publisher Copyright: {\textcopyright} 2022, Springer Science+Business Media, LLC, part of Springer Nature.",

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Use of Reduced Gravity Simulators for Plant Biological Studies. / Herranz, Raúl; Valbuena, Miguel A.; Manzano, Aránzazu et al.
Plant Gravitropism: Methods and Protocols. ed. / Elison B. Blancaflor. 2nd. ed. Humana Press Inc, 2022. p. 241-265 (Methods in Molecular Biology; Vol. 2368).

Research output: Chapter in Book / Report / Conference proceeding › Chapter › Academic › peer-review

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Use of Reduced Gravity Simulators for Plant Biological Studies

Abstract

Publication series

Bibliographical note

Funding

Keywords

Access to Document

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