Variants of the IL-10 gene associate with muscle strength in elderly from rural Africa: a candidate gene study

Karel G M Beenakker, Jacob J E Koopman, David van Bodegom, Maris Kuningas, Pieternella E Slagboom, Johannes J Meij, Andrea B Maier, Rudi G J Westendorp

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Recently, it has been shown that the capacity of the innate immune system to produce cytokines relates to skeletal muscle mass and strength in older persons. The interleukin-10 (IL-10) gene regulates the production capacities of IL-10 and tumour necrosis factor-α (TNF-α). In rural Ghana, IL-10 gene variants associated with different production capacities of IL-10 and TNF-α are enriched compared with Caucasian populations. In this setting, we explored the association between these gene variants and muscle strength. Among 554 Ghanaians aged 50 years and older, we determined 20 single nucleotide polymorphisms in the IL-10 gene, production capacities of IL-10 and TNF-α in whole blood upon stimulation with lipopolysaccharide (LPS) and handgrip strength as a proxy for skeletal muscle strength. We distinguished pro-inflammatory haplotypes associated with low IL-10 production capacity and anti-inflammatory haplotypes with high IL-10 production capacity. We found that distinct haplotypes of the IL-10 gene associated with handgrip strength. A pro-inflammatory haplotype with a population frequency of 43.2% was associated with higher handgrip strength (P = 0.015). An anti-inflammatory haplotype with a population frequency of 7.9% was associated with lower handgrip strength (P = 0.006). In conclusion, variants of the IL-10 gene contributing to a pro-inflammatory cytokine response associate with higher muscle strength, whereas those with anti-inflammatory response associate with lower muscle strength. Future research needs to elucidate whether these effects of variation in the IL-10 gene are exerted directly through its role in the repair of muscle tissue or indirectly through its role in the defence against infectious diseases.

Original languageEnglish
Pages (from-to)862-8
Number of pages7
JournalAging Cell
Volume13
Issue number5
DOIs
Publication statusPublished - Oct 2014

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Muscle Strength
Interleukin-10
Genes
Haplotypes
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha
Skeletal Muscle
Cytokines
Population
Ghana
Proxy
Single Nucleotide Polymorphism
Communicable Diseases
Lipopolysaccharides
Immune System
Muscles

Bibliographical note

© 2014 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

Keywords

  • Africa
  • Age Factors
  • Aged
  • Cytokines/genetics
  • Gene Frequency
  • Humans
  • Interleukin-10/genetics
  • Male
  • Middle Aged
  • Muscle Strength/genetics
  • Polymorphism, Single Nucleotide

Cite this

Beenakker, K. G. M., Koopman, J. J. E., van Bodegom, D., Kuningas, M., Slagboom, P. E., Meij, J. J., ... Westendorp, R. G. J. (2014). Variants of the IL-10 gene associate with muscle strength in elderly from rural Africa: a candidate gene study. Aging Cell, 13(5), 862-8. https://doi.org/10.1111/acel.12244
Beenakker, Karel G M ; Koopman, Jacob J E ; van Bodegom, David ; Kuningas, Maris ; Slagboom, Pieternella E ; Meij, Johannes J ; Maier, Andrea B ; Westendorp, Rudi G J. / Variants of the IL-10 gene associate with muscle strength in elderly from rural Africa : a candidate gene study. In: Aging Cell. 2014 ; Vol. 13, No. 5. pp. 862-8.
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title = "Variants of the IL-10 gene associate with muscle strength in elderly from rural Africa: a candidate gene study",
abstract = "Recently, it has been shown that the capacity of the innate immune system to produce cytokines relates to skeletal muscle mass and strength in older persons. The interleukin-10 (IL-10) gene regulates the production capacities of IL-10 and tumour necrosis factor-α (TNF-α). In rural Ghana, IL-10 gene variants associated with different production capacities of IL-10 and TNF-α are enriched compared with Caucasian populations. In this setting, we explored the association between these gene variants and muscle strength. Among 554 Ghanaians aged 50 years and older, we determined 20 single nucleotide polymorphisms in the IL-10 gene, production capacities of IL-10 and TNF-α in whole blood upon stimulation with lipopolysaccharide (LPS) and handgrip strength as a proxy for skeletal muscle strength. We distinguished pro-inflammatory haplotypes associated with low IL-10 production capacity and anti-inflammatory haplotypes with high IL-10 production capacity. We found that distinct haplotypes of the IL-10 gene associated with handgrip strength. A pro-inflammatory haplotype with a population frequency of 43.2{\%} was associated with higher handgrip strength (P = 0.015). An anti-inflammatory haplotype with a population frequency of 7.9{\%} was associated with lower handgrip strength (P = 0.006). In conclusion, variants of the IL-10 gene contributing to a pro-inflammatory cytokine response associate with higher muscle strength, whereas those with anti-inflammatory response associate with lower muscle strength. Future research needs to elucidate whether these effects of variation in the IL-10 gene are exerted directly through its role in the repair of muscle tissue or indirectly through its role in the defence against infectious diseases.",
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Beenakker, KGM, Koopman, JJE, van Bodegom, D, Kuningas, M, Slagboom, PE, Meij, JJ, Maier, AB & Westendorp, RGJ 2014, 'Variants of the IL-10 gene associate with muscle strength in elderly from rural Africa: a candidate gene study' Aging Cell, vol. 13, no. 5, pp. 862-8. https://doi.org/10.1111/acel.12244

Variants of the IL-10 gene associate with muscle strength in elderly from rural Africa : a candidate gene study. / Beenakker, Karel G M; Koopman, Jacob J E; van Bodegom, David; Kuningas, Maris; Slagboom, Pieternella E; Meij, Johannes J; Maier, Andrea B; Westendorp, Rudi G J.

In: Aging Cell, Vol. 13, No. 5, 10.2014, p. 862-8.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - Variants of the IL-10 gene associate with muscle strength in elderly from rural Africa

T2 - a candidate gene study

AU - Beenakker, Karel G M

AU - Koopman, Jacob J E

AU - van Bodegom, David

AU - Kuningas, Maris

AU - Slagboom, Pieternella E

AU - Meij, Johannes J

AU - Maier, Andrea B

AU - Westendorp, Rudi G J

N1 - © 2014 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

PY - 2014/10

Y1 - 2014/10

N2 - Recently, it has been shown that the capacity of the innate immune system to produce cytokines relates to skeletal muscle mass and strength in older persons. The interleukin-10 (IL-10) gene regulates the production capacities of IL-10 and tumour necrosis factor-α (TNF-α). In rural Ghana, IL-10 gene variants associated with different production capacities of IL-10 and TNF-α are enriched compared with Caucasian populations. In this setting, we explored the association between these gene variants and muscle strength. Among 554 Ghanaians aged 50 years and older, we determined 20 single nucleotide polymorphisms in the IL-10 gene, production capacities of IL-10 and TNF-α in whole blood upon stimulation with lipopolysaccharide (LPS) and handgrip strength as a proxy for skeletal muscle strength. We distinguished pro-inflammatory haplotypes associated with low IL-10 production capacity and anti-inflammatory haplotypes with high IL-10 production capacity. We found that distinct haplotypes of the IL-10 gene associated with handgrip strength. A pro-inflammatory haplotype with a population frequency of 43.2% was associated with higher handgrip strength (P = 0.015). An anti-inflammatory haplotype with a population frequency of 7.9% was associated with lower handgrip strength (P = 0.006). In conclusion, variants of the IL-10 gene contributing to a pro-inflammatory cytokine response associate with higher muscle strength, whereas those with anti-inflammatory response associate with lower muscle strength. Future research needs to elucidate whether these effects of variation in the IL-10 gene are exerted directly through its role in the repair of muscle tissue or indirectly through its role in the defence against infectious diseases.

AB - Recently, it has been shown that the capacity of the innate immune system to produce cytokines relates to skeletal muscle mass and strength in older persons. The interleukin-10 (IL-10) gene regulates the production capacities of IL-10 and tumour necrosis factor-α (TNF-α). In rural Ghana, IL-10 gene variants associated with different production capacities of IL-10 and TNF-α are enriched compared with Caucasian populations. In this setting, we explored the association between these gene variants and muscle strength. Among 554 Ghanaians aged 50 years and older, we determined 20 single nucleotide polymorphisms in the IL-10 gene, production capacities of IL-10 and TNF-α in whole blood upon stimulation with lipopolysaccharide (LPS) and handgrip strength as a proxy for skeletal muscle strength. We distinguished pro-inflammatory haplotypes associated with low IL-10 production capacity and anti-inflammatory haplotypes with high IL-10 production capacity. We found that distinct haplotypes of the IL-10 gene associated with handgrip strength. A pro-inflammatory haplotype with a population frequency of 43.2% was associated with higher handgrip strength (P = 0.015). An anti-inflammatory haplotype with a population frequency of 7.9% was associated with lower handgrip strength (P = 0.006). In conclusion, variants of the IL-10 gene contributing to a pro-inflammatory cytokine response associate with higher muscle strength, whereas those with anti-inflammatory response associate with lower muscle strength. Future research needs to elucidate whether these effects of variation in the IL-10 gene are exerted directly through its role in the repair of muscle tissue or indirectly through its role in the defence against infectious diseases.

KW - Africa

KW - Age Factors

KW - Aged

KW - Cytokines/genetics

KW - Gene Frequency

KW - Humans

KW - Interleukin-10/genetics

KW - Male

KW - Middle Aged

KW - Muscle Strength/genetics

KW - Polymorphism, Single Nucleotide

U2 - 10.1111/acel.12244

DO - 10.1111/acel.12244

M3 - Article

VL - 13

SP - 862

EP - 868

JO - Aging Cell

JF - Aging Cell

SN - 1474-9718

IS - 5

ER -

Beenakker KGM, Koopman JJE, van Bodegom D, Kuningas M, Slagboom PE, Meij JJ et al. Variants of the IL-10 gene associate with muscle strength in elderly from rural Africa: a candidate gene study. Aging Cell. 2014 Oct;13(5):862-8. https://doi.org/10.1111/acel.12244