Versatility of inner membrane protein biogenesis in Escherichia coli.

L. Froderberg, E.N.G. Houben, J.C. Samuleson, M. Chen, S.K. Park, G.J. Philips, R.E. Dalbey, S. Luirink, J.-W. de Gier

Research output: Contribution to JournalArticleAcademic

Abstract

To further our understanding of inner membrane protein (IMP) biogenesis in Escherichia coli, we have accomplished the widest in vivo IMP assembly screen so far. The biogenesis of a set of model IMPs covering most IMP structures possible has been studied in a variety of signal recognition particle (SRP), Sec and YidC mutant strains. We show that the assembly of the complete set of model IMPs is assisted (i.e. requires the aid of proteinaceous factors), and that the requirements for assembly of the model IMPs into the inner membrane differ significantly from each other. This indicates that IMP assembly is much more versatile than previously thought.
Original languageEnglish
Pages (from-to)1015-1027
Number of pages13
JournalMolecular Microbiology
Volume47
DOIs
Publication statusPublished - 2003

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Inosine Monophosphate
Membrane Proteins
Escherichia coli
Signal Recognition Particle
Membranes

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Froderberg, L., Houben, E. N. G., Samuleson, J. C., Chen, M., Park, S. K., Philips, G. J., ... de Gier, J-W. (2003). Versatility of inner membrane protein biogenesis in Escherichia coli. Molecular Microbiology, 47, 1015-1027. https://doi.org/10.1046/j.1365-2958.2003.03346.x
Froderberg, L. ; Houben, E.N.G. ; Samuleson, J.C. ; Chen, M. ; Park, S.K. ; Philips, G.J. ; Dalbey, R.E. ; Luirink, S. ; de Gier, J.-W. / Versatility of inner membrane protein biogenesis in Escherichia coli. In: Molecular Microbiology. 2003 ; Vol. 47. pp. 1015-1027.
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Froderberg, L, Houben, ENG, Samuleson, JC, Chen, M, Park, SK, Philips, GJ, Dalbey, RE, Luirink, S & de Gier, J-W 2003, 'Versatility of inner membrane protein biogenesis in Escherichia coli.' Molecular Microbiology, vol. 47, pp. 1015-1027. https://doi.org/10.1046/j.1365-2958.2003.03346.x

Versatility of inner membrane protein biogenesis in Escherichia coli. / Froderberg, L.; Houben, E.N.G.; Samuleson, J.C.; Chen, M.; Park, S.K.; Philips, G.J.; Dalbey, R.E.; Luirink, S.; de Gier, J.-W.

In: Molecular Microbiology, Vol. 47, 2003, p. 1015-1027.

Research output: Contribution to JournalArticleAcademic

TY - JOUR

T1 - Versatility of inner membrane protein biogenesis in Escherichia coli.

AU - Froderberg, L.

AU - Houben, E.N.G.

AU - Samuleson, J.C.

AU - Chen, M.

AU - Park, S.K.

AU - Philips, G.J.

AU - Dalbey, R.E.

AU - Luirink, S.

AU - de Gier, J.-W.

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AB - To further our understanding of inner membrane protein (IMP) biogenesis in Escherichia coli, we have accomplished the widest in vivo IMP assembly screen so far. The biogenesis of a set of model IMPs covering most IMP structures possible has been studied in a variety of signal recognition particle (SRP), Sec and YidC mutant strains. We show that the assembly of the complete set of model IMPs is assisted (i.e. requires the aid of proteinaceous factors), and that the requirements for assembly of the model IMPs into the inner membrane differ significantly from each other. This indicates that IMP assembly is much more versatile than previously thought.

U2 - 10.1046/j.1365-2958.2003.03346.x

DO - 10.1046/j.1365-2958.2003.03346.x

M3 - Article

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SP - 1015

EP - 1027

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

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