Vesicle trafficking via the Spitzenkoerper during hyphal tip growth in Rhizoctonia solani

J. Dijksterhuis, D. Molenaar

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Growing hyphae of Rhizoctonia solani were stained with the endocytic marker dye FM4-64 and imaged by confocal microscopy. Staining of the plasma membrane was followed by labeling of organelles in the cytoplasm (after ~1 min) and of the Spitzenkörper (Spk; after ~2 min). Fluorescence recovery after photobleaching (FRAP) of the stained Spk demonstrated the vectorial flow of secretory vesicles from the apical cytoplasm to the Spk. This flux was modelled in a two-compartment model. The turnover time of the vesicles of the Spk was estimated to be 1.3-2.5 min. These results are roughly consistent with the expected flux of vesicles through the Spk based on the number of secretory vesicles within the Spk and the number of secretory vesicles that would be necessary to fuse with the apical plasma membrane to maintain hyphal extension rates. These results suggest that membrane retrieval via endocytosis is not as significant as previously suggested. © 2013 Springer Science+Business Media Dordrecht.
Original languageEnglish
Pages (from-to)921-931
JournalAntonie van Leeuwenhoek
Volume103
DOIs
Publication statusPublished - 2013

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Rhizoctonia
Secretory Vesicles
Cytoplasm
Growth
Cell Membrane
Fluorescence Recovery After Photobleaching
Hyphae
Endocytosis
Confocal Microscopy
Organelles
Coloring Agents
Staining and Labeling
Membranes

Cite this

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title = "Vesicle trafficking via the Spitzenkoerper during hyphal tip growth in Rhizoctonia solani",
abstract = "Growing hyphae of Rhizoctonia solani were stained with the endocytic marker dye FM4-64 and imaged by confocal microscopy. Staining of the plasma membrane was followed by labeling of organelles in the cytoplasm (after ~1 min) and of the Spitzenk{\"o}rper (Spk; after ~2 min). Fluorescence recovery after photobleaching (FRAP) of the stained Spk demonstrated the vectorial flow of secretory vesicles from the apical cytoplasm to the Spk. This flux was modelled in a two-compartment model. The turnover time of the vesicles of the Spk was estimated to be 1.3-2.5 min. These results are roughly consistent with the expected flux of vesicles through the Spk based on the number of secretory vesicles within the Spk and the number of secretory vesicles that would be necessary to fuse with the apical plasma membrane to maintain hyphal extension rates. These results suggest that membrane retrieval via endocytosis is not as significant as previously suggested. {\circledC} 2013 Springer Science+Business Media Dordrecht.",
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Vesicle trafficking via the Spitzenkoerper during hyphal tip growth in Rhizoctonia solani. / Dijksterhuis, J.; Molenaar, D.

In: Antonie van Leeuwenhoek, Vol. 103, 2013, p. 921-931.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - Vesicle trafficking via the Spitzenkoerper during hyphal tip growth in Rhizoctonia solani

AU - Dijksterhuis, J.

AU - Molenaar, D.

PY - 2013

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N2 - Growing hyphae of Rhizoctonia solani were stained with the endocytic marker dye FM4-64 and imaged by confocal microscopy. Staining of the plasma membrane was followed by labeling of organelles in the cytoplasm (after ~1 min) and of the Spitzenkörper (Spk; after ~2 min). Fluorescence recovery after photobleaching (FRAP) of the stained Spk demonstrated the vectorial flow of secretory vesicles from the apical cytoplasm to the Spk. This flux was modelled in a two-compartment model. The turnover time of the vesicles of the Spk was estimated to be 1.3-2.5 min. These results are roughly consistent with the expected flux of vesicles through the Spk based on the number of secretory vesicles within the Spk and the number of secretory vesicles that would be necessary to fuse with the apical plasma membrane to maintain hyphal extension rates. These results suggest that membrane retrieval via endocytosis is not as significant as previously suggested. © 2013 Springer Science+Business Media Dordrecht.

AB - Growing hyphae of Rhizoctonia solani were stained with the endocytic marker dye FM4-64 and imaged by confocal microscopy. Staining of the plasma membrane was followed by labeling of organelles in the cytoplasm (after ~1 min) and of the Spitzenkörper (Spk; after ~2 min). Fluorescence recovery after photobleaching (FRAP) of the stained Spk demonstrated the vectorial flow of secretory vesicles from the apical cytoplasm to the Spk. This flux was modelled in a two-compartment model. The turnover time of the vesicles of the Spk was estimated to be 1.3-2.5 min. These results are roughly consistent with the expected flux of vesicles through the Spk based on the number of secretory vesicles within the Spk and the number of secretory vesicles that would be necessary to fuse with the apical plasma membrane to maintain hyphal extension rates. These results suggest that membrane retrieval via endocytosis is not as significant as previously suggested. © 2013 Springer Science+Business Media Dordrecht.

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