Visible Diode Laser-Induced Fluorescence Detection in Liquid Chromatography after Precolumn Derivatization of Amines.

A.J.G. Mank; H.T.C. van der Laan; H. Lingeman; C. Gooijer

doi:10.1021/ac00106a015

Visible Diode Laser-Induced Fluorescence Detection in Liquid Chromatography after Precolumn Derivatization of Amines.

A.J.G. Mank, H.T.C. van der Laan, H. Lingeman, C. Gooijer

BioAnalytical Chemistry

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

To fully exploit the attractive visible diode laser-induced fluorescence (LIF) detection technique in column liquid chromatography (LC), covalent labeling procedures need to be developed which are compatible with near-infrared fluorescence. For this purpose, several red-absorbing labels containing a single succinimidyl ester functionality were synthesized and used for the derivatization of primary and secondary amines. Oxazines/thiazines, squaraines, and dicarbocyanines were examined as redabsorbing fluorophores. The quality of the LC-diode LIF system for the analytes concerned is illustrated by the concentration detection limit of the labeled n-octylamine (a test compound), which was as low as 2 x 10

Original language	English
Pages (from-to)	1742
Journal	Analytical Chemistry
Volume	67
DOIs	https://doi.org/10.1021/ac00106a015
Publication status	Published - 1995

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title = "Visible Diode Laser-Induced Fluorescence Detection in Liquid Chromatography after Precolumn Derivatization of Amines.",

abstract = "To fully exploit the attractive visible diode laser-induced fluorescence (LIF) detection technique in column liquid chromatography (LC), covalent labeling procedures need to be developed which are compatible with near-infrared fluorescence. For this purpose, several red-absorbing labels containing a single succinimidyl ester functionality were synthesized and used for the derivatization of primary and secondary amines. Oxazines/thiazines, squaraines, and dicarbocyanines were examined as redabsorbing fluorophores. The quality of the LC-diode LIF system for the analytes concerned is illustrated by the concentration detection limit of the labeled n-octylamine (a test compound), which was as low as 2 x 10",

author = "A.J.G. Mank and {van der Laan}, H.T.C. and H. Lingeman and C. Gooijer",

year = "1995",

doi = "10.1021/ac00106a015",

language = "English",

volume = "67",

pages = "1742",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

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T1 - Visible Diode Laser-Induced Fluorescence Detection in Liquid Chromatography after Precolumn Derivatization of Amines.

AU - Mank, A.J.G.

AU - van der Laan, H.T.C.

AU - Lingeman, H.

AU - Gooijer, C.

PY - 1995

Y1 - 1995

N2 - To fully exploit the attractive visible diode laser-induced fluorescence (LIF) detection technique in column liquid chromatography (LC), covalent labeling procedures need to be developed which are compatible with near-infrared fluorescence. For this purpose, several red-absorbing labels containing a single succinimidyl ester functionality were synthesized and used for the derivatization of primary and secondary amines. Oxazines/thiazines, squaraines, and dicarbocyanines were examined as redabsorbing fluorophores. The quality of the LC-diode LIF system for the analytes concerned is illustrated by the concentration detection limit of the labeled n-octylamine (a test compound), which was as low as 2 x 10

AB - To fully exploit the attractive visible diode laser-induced fluorescence (LIF) detection technique in column liquid chromatography (LC), covalent labeling procedures need to be developed which are compatible with near-infrared fluorescence. For this purpose, several red-absorbing labels containing a single succinimidyl ester functionality were synthesized and used for the derivatization of primary and secondary amines. Oxazines/thiazines, squaraines, and dicarbocyanines were examined as redabsorbing fluorophores. The quality of the LC-diode LIF system for the analytes concerned is illustrated by the concentration detection limit of the labeled n-octylamine (a test compound), which was as low as 2 x 10

U2 - 10.1021/ac00106a015

DO - 10.1021/ac00106a015

M3 - Article

VL - 67

SP - 1742

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

ER -

Visible Diode Laser-Induced Fluorescence Detection in Liquid Chromatography after Precolumn Derivatization of Amines.

Abstract

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