Visible Diode Laser-Induced Fluorescence Detection in Liquid Chromatography after Precolumn Derivatization of Amines.

A.J.G. Mank, H.T.C. van der Laan, H. Lingeman, C. Gooijer

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

To fully exploit the attractive visible diode laser-induced fluorescence (LIF) detection technique in column liquid chromatography (LC), covalent labeling procedures need to be developed which are compatible with near-infrared fluorescence. For this purpose, several red-absorbing labels containing a single succinimidyl ester functionality were synthesized and used for the derivatization of primary and secondary amines. Oxazines/thiazines, squaraines, and dicarbocyanines were examined as redabsorbing fluorophores. The quality of the LC-diode LIF system for the analytes concerned is illustrated by the concentration detection limit of the labeled n-octylamine (a test compound), which was as low as 2 x 10
Original languageEnglish
Pages (from-to)1742
JournalAnalytical Chemistry
Volume67
DOIs
Publication statusPublished - 1995

Fingerprint

Dive into the research topics of 'Visible Diode Laser-Induced Fluorescence Detection in Liquid Chromatography after Precolumn Derivatization of Amines.'. Together they form a unique fingerprint.

Cite this