Visualizing resonance energy transfer in supramolecular surface patterns of β-CD-functionalized quantum dot hosts and organic dye guests by fluorescence lifetime imaging

Denis Dorokhin, Shu-Han Hsu, Nikodem Tomczak, Christian Blum, Vinod Subramaniam, Jurriaan Huskens, David N Reinhoudt, Aldrik H Velders, G Julius Vancso

    Research output: Contribution to JournalArticleAcademicpeer-review

    Abstract

    Detection of an analyte via supramolecular host-guest binding and quantum dot (QD)-based fluorescence resonance energy transfer (FRET) signal transduction mechanism is demonstrated. Surface patterns consisting of CdSe/ZnS QDs functionalized at their periphery with β-cyclodextrin (β-CD) were obtained by immobilization of the QDs from solution onto glass substrates patterned with adamantyl-terminated poly(propylene imine) dendrimeric "glue." Subsequent formation of host-guest complexes between vacant β-CD on the QD surface and an adamantyl-functionalized lissamine rhodamine resulting in FRET was confirmed by fluorescence microscopy, spectroscopy, and fluorescence lifetime imaging microscopy (FLIM).

    Original languageEnglish
    Pages (from-to)2870-6
    Number of pages7
    JournalSmall
    Volume6
    Issue number24
    DOIs
    Publication statusPublished - 20 Dec 2010

    Keywords

    • Fluorescence Resonance Energy Transfer
    • Microscopy, Fluorescence
    • Quantum Dots
    • beta-Cyclodextrins
    • Journal Article
    • Research Support, Non-U.S. Gov't

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