Visualizing resonance energy transfer in supramolecular surface patterns of β-CD-functionalized quantum dot hosts and organic dye guests by fluorescence lifetime imaging

Denis Dorokhin; Shu-Han Hsu; Nikodem Tomczak; Christian Blum; Vinod Subramaniam; Jurriaan Huskens; David N Reinhoudt; Aldrik H Velders; G Julius Vancso

doi:10.1002/smll.201000713

Visualizing resonance energy transfer in supramolecular surface patterns of β-CD-functionalized quantum dot hosts and organic dye guests by fluorescence lifetime imaging

Denis Dorokhin, Shu-Han Hsu, Nikodem Tomczak, Christian Blum, Vinod Subramaniam, Jurriaan Huskens, David N Reinhoudt, Aldrik H Velders, G Julius Vancso

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Detection of an analyte via supramolecular host-guest binding and quantum dot (QD)-based fluorescence resonance energy transfer (FRET) signal transduction mechanism is demonstrated. Surface patterns consisting of CdSe/ZnS QDs functionalized at their periphery with β-cyclodextrin (β-CD) were obtained by immobilization of the QDs from solution onto glass substrates patterned with adamantyl-terminated poly(propylene imine) dendrimeric "glue." Subsequent formation of host-guest complexes between vacant β-CD on the QD surface and an adamantyl-functionalized lissamine rhodamine resulting in FRET was confirmed by fluorescence microscopy, spectroscopy, and fluorescence lifetime imaging microscopy (FLIM).

Original language	English
Pages (from-to)	2870-6
Number of pages	7
Journal	Small
Volume	6
Issue number	24
DOIs	https://doi.org/10.1002/smll.201000713
Publication status	Published - 20 Dec 2010

Keywords

Fluorescence Resonance Energy Transfer
Microscopy, Fluorescence
Quantum Dots
beta-Cyclodextrins
Journal Article
Research Support, Non-U.S. Gov't

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/smll.201000713

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title = "Visualizing resonance energy transfer in supramolecular surface patterns of β-CD-functionalized quantum dot hosts and organic dye guests by fluorescence lifetime imaging",

abstract = "Detection of an analyte via supramolecular host-guest binding and quantum dot (QD)-based fluorescence resonance energy transfer (FRET) signal transduction mechanism is demonstrated. Surface patterns consisting of CdSe/ZnS QDs functionalized at their periphery with β-cyclodextrin (β-CD) were obtained by immobilization of the QDs from solution onto glass substrates patterned with adamantyl-terminated poly(propylene imine) dendrimeric {"}glue.{"} Subsequent formation of host-guest complexes between vacant β-CD on the QD surface and an adamantyl-functionalized lissamine rhodamine resulting in FRET was confirmed by fluorescence microscopy, spectroscopy, and fluorescence lifetime imaging microscopy (FLIM).",

keywords = "Fluorescence Resonance Energy Transfer, Microscopy, Fluorescence, Quantum Dots, beta-Cyclodextrins, Journal Article, Research Support, Non-U.S. Gov't",

author = "Denis Dorokhin and Shu-Han Hsu and Nikodem Tomczak and Christian Blum and Vinod Subramaniam and Jurriaan Huskens and Reinhoudt, {David N} and Velders, {Aldrik H} and Vancso, {G Julius}",

year = "2010",

month = dec,

day = "20",

doi = "10.1002/smll.201000713",

language = "English",

volume = "6",

pages = "2870--6",

journal = "Small",

issn = "1613-6810",

publisher = "Wiley-VCH Verlag",

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TY - JOUR

T1 - Visualizing resonance energy transfer in supramolecular surface patterns of β-CD-functionalized quantum dot hosts and organic dye guests by fluorescence lifetime imaging

AU - Dorokhin, Denis

AU - Hsu, Shu-Han

AU - Tomczak, Nikodem

AU - Blum, Christian

AU - Subramaniam, Vinod

AU - Huskens, Jurriaan

AU - Reinhoudt, David N

AU - Velders, Aldrik H

AU - Vancso, G Julius

PY - 2010/12/20

Y1 - 2010/12/20

N2 - Detection of an analyte via supramolecular host-guest binding and quantum dot (QD)-based fluorescence resonance energy transfer (FRET) signal transduction mechanism is demonstrated. Surface patterns consisting of CdSe/ZnS QDs functionalized at their periphery with β-cyclodextrin (β-CD) were obtained by immobilization of the QDs from solution onto glass substrates patterned with adamantyl-terminated poly(propylene imine) dendrimeric "glue." Subsequent formation of host-guest complexes between vacant β-CD on the QD surface and an adamantyl-functionalized lissamine rhodamine resulting in FRET was confirmed by fluorescence microscopy, spectroscopy, and fluorescence lifetime imaging microscopy (FLIM).

AB - Detection of an analyte via supramolecular host-guest binding and quantum dot (QD)-based fluorescence resonance energy transfer (FRET) signal transduction mechanism is demonstrated. Surface patterns consisting of CdSe/ZnS QDs functionalized at their periphery with β-cyclodextrin (β-CD) were obtained by immobilization of the QDs from solution onto glass substrates patterned with adamantyl-terminated poly(propylene imine) dendrimeric "glue." Subsequent formation of host-guest complexes between vacant β-CD on the QD surface and an adamantyl-functionalized lissamine rhodamine resulting in FRET was confirmed by fluorescence microscopy, spectroscopy, and fluorescence lifetime imaging microscopy (FLIM).

KW - Fluorescence Resonance Energy Transfer

KW - Microscopy, Fluorescence

KW - Quantum Dots

KW - beta-Cyclodextrins

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1002/smll.201000713

DO - 10.1002/smll.201000713

M3 - Article

C2 - 21080386

SN - 1613-6810

VL - 6

SP - 2870

EP - 2876

JO - Small

JF - Small

IS - 24

ER -

Visualizing resonance energy transfer in supramolecular surface patterns of β-CD-functionalized quantum dot hosts and organic dye guests by fluorescence lifetime imaging

Abstract

Keywords

UN SDGs

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