Voids and marginal gaps in oval-shaped root canals filled with calcium silicate-based sealers: Micro-CT study

Isabella Marian Lena; Cristiana Pereira Malta; Tamyres Veleda Fonseca; João Paulo Mendes Tribst; Amanda Maria de Oliveira Dal Piva; Cornelis Johannes Kleverlaan; János Kodolányi; Gabriel Kalil Rocha Pereira; Patrícia Maria Poli Kopper Móra; Renata Dornelles Morgental

doi:10.1590/0103-644020256732

Voids and marginal gaps in oval-shaped root canals filled with calcium silicate-based sealers: Micro-CT study

Isabella Marian Lena, Cristiana Pereira Malta, Tamyres Veleda Fonseca, João Paulo Mendes Tribst, Amanda Maria de Oliveira Dal Piva, Cornelis Johannes Kleverlaan, János Kodolányi, Gabriel Kalil Rocha Pereira, Patrícia Maria Poli Kopper Móra, Renata Dornelles Morgental

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

This study investigated the percentage of voids and marginal gaps in oval root canals filled with pre-mixed or powder-liquid calcium silicate-based sealers, using the single-cone technique. Eighty-four mandibular anterior teeth with oval root canals were selected and prepared by Reciproc R25 instruments. Teeth were then divided into two groups based on the sealer: Pre-mixed (BCS, Bio-C Sealer) (n=42) or Manipulated (BRR, BioRoot RCS) (n=42). Microcomputed tomography (micro-CT) was used to evaluate the percentage of voids and marginal gaps in the apical, middle, and cervical thirds of the root canals. Surface morphology of each sealer was evaluated on bovine dentin discs by scanning electron microscopy (SEM). Mann-Whitney and Friedman tests were employed for statistical analysis (α=5). BCS presented lower percentages of marginal gaps (p=0.00) and overall empty spaces (voids + gaps, p=0.01) compared to BRR. While BRR exhibited the lowest percentage of marginal gaps in the cervical third (p=0.00), BCS showed consistent performance across all root thirds (p>0.05). SEM showed that BCS has a homogeneous structure and compact structure, while BRR exhibits a more porous surface with a greater number of mostly micron-sized or larger voids. BCS exhibited superior filling ability in oval root canals compared to BRR. The BRR group showed the highest percentage of gaps in the middle and apical segments.

Original language	English
Article number	e25-6732
Pages (from-to)	1-11
Number of pages	11
Journal	Brazilian Dental Journal
Volume	36
DOIs	https://doi.org/10.1590/0103-644020256732
Publication status	Published - 2025

Bibliographical note

Published online: 12 January 2026

Funding

This work is part of fulfilling the requirements of the Ph.D. degree (I.M.L) in the Post-Graduate Program in Oral Sciences at the Faculty of Dentistry, Federal University of Santa Maria (Rio Grande do Sul State, Brazil). Besides, this study was partially financed by the Brazilian Federal Agency for Coordination of Improvement of Higher Education Personnel – CAPES (Finance code 001, I.M.L. Doctorate's scholarship and abroad visiting-researcher scholarship #88887.891846/2023-00 at CAPES/PrInt Program, Smart Materials Project), and by the Brazilian National Council for Scientific and Technological Development (CNPq) (G.K.R.P. research grant, #304665/2022-3). We emphasize that the institutions above had no role in the study design, data collection, or analysis, the decision to publish, or the preparation of the manuscript. This work is part of fulfilling the requirements of the Ph.D. degree (I.M.L) in the Post-Graduate Program in Oral Sciences at the Faculty of Dentistry, Federal University of Santa Maria (Rio Grande do Sul State, Brazil). Besides, this study was partially financed by the Brazilian Federal Agency for Coordination of Improvement of Higher Education Personnel ? CAPES (Finance code 001, I.M.L. Doctorate's scholarship and abroad visiting-researcher scholarship #88887.891846/2023-00 at CAPES/PrInt Program, Smart Materials Project), and by the Brazilian National Council for Scientific and Technological Development (CNPq) (G.K.R.P. research grant, #304665/2022-3). We emphasize that the institutions above had no role in the study design, data collection, or analysis, the decision to publish, or the preparation of the manuscript.

Funders	Funder number
Universidade Federal de Santa Maria
PRINT
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior	88887.891846/2023-00
Conselho Nacional de Desenvolvimento Científico e Tecnológico	304665/2022-3

Access to Document

10.1590/0103-644020256732

Persistent URL (handle)

Persistent link

Cite this

Lena, I. M., Malta, C. P., Fonseca, T. V., Tribst, J. P. M., Piva, A. M. D. O. D., Kleverlaan, C. J., Kodolányi, J., Pereira, G. K. R., Móra, P. M. P. K., & Morgental, R. D. (2025). Voids and marginal gaps in oval-shaped root canals filled with calcium silicate-based sealers: Micro-CT study. Brazilian Dental Journal, 36, 1-11. Article e25-6732. https://doi.org/10.1590/0103-644020256732

@article{1518f5b028ee45479cfffee08d7b5ed7,

title = "Voids and marginal gaps in oval-shaped root canals filled with calcium silicate-based sealers: Micro-CT study",

abstract = "This study investigated the percentage of voids and marginal gaps in oval root canals filled with pre-mixed or powder-liquid calcium silicate-based sealers, using the single-cone technique. Eighty-four mandibular anterior teeth with oval root canals were selected and prepared by Reciproc R25 instruments. Teeth were then divided into two groups based on the sealer: Pre-mixed (BCS, Bio-C Sealer) (n=42) or Manipulated (BRR, BioRoot RCS) (n=42). Microcomputed tomography (micro-CT) was used to evaluate the percentage of voids and marginal gaps in the apical, middle, and cervical thirds of the root canals. Surface morphology of each sealer was evaluated on bovine dentin discs by scanning electron microscopy (SEM). Mann-Whitney and Friedman tests were employed for statistical analysis (α=5). BCS presented lower percentages of marginal gaps (p=0.00) and overall empty spaces (voids + gaps, p=0.01) compared to BRR. While BRR exhibited the lowest percentage of marginal gaps in the cervical third (p=0.00), BCS showed consistent performance across all root thirds (p>0.05). SEM showed that BCS has a homogeneous structure and compact structure, while BRR exhibits a more porous surface with a greater number of mostly micron-sized or larger voids. BCS exhibited superior filling ability in oval root canals compared to BRR. The BRR group showed the highest percentage of gaps in the middle and apical segments.",

author = "Lena, \{Isabella Marian\} and Malta, \{Cristiana Pereira\} and Fonseca, \{Tamyres Veleda\} and Tribst, \{Jo{\~a}o Paulo Mendes\} and Piva, \{Amanda Maria de Oliveira Dal\} and Kleverlaan, \{Cornelis Johannes\} and J{\'a}nos Kodol{\'a}nyi and Pereira, \{Gabriel Kalil Rocha\} and M{\'o}ra, \{Patr{\'i}cia Maria Poli Kopper\} and Morgental, \{Renata Dornelles\}",

note = "Published online: 12 January 2026",

year = "2025",

doi = "10.1590/0103-644020256732",

language = "English",

volume = "36",

pages = "1--11",

journal = "Brazilian Dental Journal",

issn = "0103-6440",

publisher = "Associacao Brasileira de Divulgacao Cientifica",

}

TY - JOUR

T1 - Voids and marginal gaps in oval-shaped root canals filled with calcium silicate-based sealers

T2 - Micro-CT study

AU - Lena, Isabella Marian

AU - Malta, Cristiana Pereira

AU - Fonseca, Tamyres Veleda

AU - Tribst, João Paulo Mendes

AU - Piva, Amanda Maria de Oliveira Dal

AU - Kleverlaan, Cornelis Johannes

AU - Kodolányi, János

AU - Pereira, Gabriel Kalil Rocha

AU - Móra, Patrícia Maria Poli Kopper

AU - Morgental, Renata Dornelles

N1 - Published online: 12 January 2026

PY - 2025

Y1 - 2025

N2 - This study investigated the percentage of voids and marginal gaps in oval root canals filled with pre-mixed or powder-liquid calcium silicate-based sealers, using the single-cone technique. Eighty-four mandibular anterior teeth with oval root canals were selected and prepared by Reciproc R25 instruments. Teeth were then divided into two groups based on the sealer: Pre-mixed (BCS, Bio-C Sealer) (n=42) or Manipulated (BRR, BioRoot RCS) (n=42). Microcomputed tomography (micro-CT) was used to evaluate the percentage of voids and marginal gaps in the apical, middle, and cervical thirds of the root canals. Surface morphology of each sealer was evaluated on bovine dentin discs by scanning electron microscopy (SEM). Mann-Whitney and Friedman tests were employed for statistical analysis (α=5). BCS presented lower percentages of marginal gaps (p=0.00) and overall empty spaces (voids + gaps, p=0.01) compared to BRR. While BRR exhibited the lowest percentage of marginal gaps in the cervical third (p=0.00), BCS showed consistent performance across all root thirds (p>0.05). SEM showed that BCS has a homogeneous structure and compact structure, while BRR exhibits a more porous surface with a greater number of mostly micron-sized or larger voids. BCS exhibited superior filling ability in oval root canals compared to BRR. The BRR group showed the highest percentage of gaps in the middle and apical segments.

AB - This study investigated the percentage of voids and marginal gaps in oval root canals filled with pre-mixed or powder-liquid calcium silicate-based sealers, using the single-cone technique. Eighty-four mandibular anterior teeth with oval root canals were selected and prepared by Reciproc R25 instruments. Teeth were then divided into two groups based on the sealer: Pre-mixed (BCS, Bio-C Sealer) (n=42) or Manipulated (BRR, BioRoot RCS) (n=42). Microcomputed tomography (micro-CT) was used to evaluate the percentage of voids and marginal gaps in the apical, middle, and cervical thirds of the root canals. Surface morphology of each sealer was evaluated on bovine dentin discs by scanning electron microscopy (SEM). Mann-Whitney and Friedman tests were employed for statistical analysis (α=5). BCS presented lower percentages of marginal gaps (p=0.00) and overall empty spaces (voids + gaps, p=0.01) compared to BRR. While BRR exhibited the lowest percentage of marginal gaps in the cervical third (p=0.00), BCS showed consistent performance across all root thirds (p>0.05). SEM showed that BCS has a homogeneous structure and compact structure, while BRR exhibits a more porous surface with a greater number of mostly micron-sized or larger voids. BCS exhibited superior filling ability in oval root canals compared to BRR. The BRR group showed the highest percentage of gaps in the middle and apical segments.

UR - https://www.scopus.com/pages/publications/105027606038

UR - https://www.scopus.com/inward/citedby.url?scp=105027606038&partnerID=8YFLogxK

U2 - 10.1590/0103-644020256732

DO - 10.1590/0103-644020256732

M3 - Article

C2 - 41538499

AN - SCOPUS:105027606038

SN - 0103-6440

VL - 36

SP - 1

EP - 11

JO - Brazilian Dental Journal

JF - Brazilian Dental Journal

M1 - e25-6732

ER -

Voids and marginal gaps in oval-shaped root canals filled with calcium silicate-based sealers: Micro-CT study

Abstract

Bibliographical note

Funding

Access to Document

Persistent URL (handle)

Other files and links

Fingerprint

Cite this