"Watching the Detectives" report of the general assembly of the EU project DETECTIVE Brussels, 24-25 November 2015

Ruani N Fernando, Umesh Chaudhari, Sylvia E Escher, Jan G. Hengstler, Jürgen Hescheler, Paul Jennings, Hector C Keun, Jos C S Kleinjans, Raivo Kolde, Laxmikanth Kollipara, Annette Kopp-Schneider, Alice Limonciel, Harshal Nemade, Filomain Nguemo, Hedi Peterson, Pilar Prieto, Robim M Rodrigues, Agapios Sachinidis, Christoph Schäfer, Albert SickmannDimitry Spitkovsky, Regina Stöber, Simone G J van Breda, Bob van de Water, Manon Vivier, René P Zahedi, Mathieu Vinken, Vera Rogiers

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

SEURAT-1 is a joint research initiative between the European Commission and Cosmetics Europe aiming to develop in vitro- and in silico-based methods to replace the in vivo repeated dose systemic toxicity test used for the assessment of human safety. As one of the building blocks of SEURAT-1, the DETECTIVE project focused on a key element on which in vitro toxicity testing relies: the development of robust and reliable, sensitive and specific in vitro biomarkers and surrogate endpoints that can be used for safety assessments of chronically acting toxicants, relevant for humans. The work conducted by the DETECTIVE consortium partners has established a screening pipeline of functional and "-omics" technologies, including high-content and high-throughput screening platforms, to develop and investigate human biomarkers for repeated dose toxicity in cellular in vitro models. Identification and statistical selection of highly predictive biomarkers in a pathway- and evidence-based approach constitute a major step in an integrated approach towards the replacement of animal testing in human safety assessment. To discuss the final outcomes and achievements of the consortium, a meeting was organized in Brussels. This meeting brought together data-producing and supporting consortium partners. The presentations focused on the current state of ongoing and concluding projects and the strategies employed to identify new relevant biomarkers of toxicity. The outcomes and deliverables, including the dissemination of results in data-rich "-omics" databases, were discussed as were the future perspectives of the work completed under the DETECTIVE project. Although some projects were still in progress and required continued data analysis, this report summarizes the presentations, discussions and the outcomes of the project.

Original languageEnglish
Pages (from-to)1529-1539
Number of pages11
JournalArchives of Toxicology
Volume90
Issue number6
DOIs
Publication statusPublished - Jun 2016
Externally publishedYes

Keywords

  • Animal Testing Alternatives
  • Animals
  • Biomarkers
  • Cells, Cultured
  • Consumer Product Safety
  • European Union
  • Government Regulation
  • High-Throughput Screening Assays
  • Humans
  • In Vitro Techniques
  • Toxicity Tests
  • Congresses

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