Zinc- and nickel-induced changes in fatty acid profiles in the zinc hyperaccumulator Arabidopsis halleri and non-accumulator Arabidopsis lyrata

Ilya V. Seregin; Tatiana V. Ivanova; Alexander S. Voronkov; Anna D. Kozhevnikova; Henk Schat

doi:10.1016/j.plaphy.2023.107640

Zinc- and nickel-induced changes in fatty acid profiles in the zinc hyperaccumulator Arabidopsis halleri and non-accumulator Arabidopsis lyrata

Ilya V. Seregin^*, Tatiana V. Ivanova, Alexander S. Voronkov, Anna D. Kozhevnikova, Henk Schat

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

This pilot study aimed at comparing zinc (Zn) and nickel (Ni) effects on the fatty acid (FA) profiles, oxidative stress and desaturase activity in the Zn hyperaccumulator Arabidopsis halleri and the excluder Arabidopsis lyrata to allow a better picture of the physiological mechanisms which may contribute to metal tolerance or acclimation. The most significant changes in the FA composition were observed in the shoots of the hyperaccumulator and in the roots of the excluder, and were not only metal-dependent, but also species-specific, since the most significant changes in the shoots of A. halleri were observed under Ni treatment, though Ni, in contrast to Zn, was accumulated mainly in its roots. Several FAs appeared in the roots and shoots of A. lyrata only upon metal exposure, whereas they were already found in control A. halleri. In both species, there was an increase in oleic acid under Ni treatment in both organs, whereas in Zn-treated plants the increase was shown only for the shoots. A rare conjugated α-parinaric acid was identified only in the shoots of metal-treated A. halleri. In the shoots of the hyperaccumulator, there was an increase in the content of saturated FAs and a decrease in the content of unsaturated FAs, while in the roots of the excluder, the opposite pattern was observed. These metal-induced changes in FA composition in the shoots of A. halleri can lead to a decrease in the fluidity of membranes, which could diminish the penetration of ROS into the membrane and thus maintain its stability.

Original language	English
Article number	107640
Pages (from-to)	1-21
Number of pages	21
Journal	Plant Physiology and Biochemistry
Volume	197
Early online date	15 Mar 2023
DOIs	https://doi.org/10.1016/j.plaphy.2023.107640
Publication status	Published - Apr 2023

Bibliographical note

Funding Information:
This work was supported by the Russian Science Foundation grant № 21-14-00028 ( https://rscf.ru/project/21-14-00028/ ) (studies of Ni and Zn effects on FA profiles, the UI, desaturase activity, and MDA concentration, as well as Ni and Zn accumulation in plant roots and shoots, metal translocation and total uptake). Spectroscopic analysis ( Fig. S1 ) was partially supported by the Ministry of Science and Higher Education of the Russian Federation , state assignments № 122042700044–6 , 122042700043–9 . The authors thank Prof. M. S. Trofimova and N. K. Lapshin for their assistance with measuring the absorption and fluorescence emission spectra and Prof. Hélène Frérot-Pauwels, Université Lille1, for donating the seeds of Arabidopsis lyrata and Arabidopsis halleri.

Funding Information:
This work was supported by the Russian Science Foundation grant № 21-14-00028 (https://rscf.ru/project/21-14-00028/) (studies of Ni and Zn effects on FA profiles, the UI, desaturase activity, and MDA concentration, as well as Ni and Zn accumulation in plant roots and shoots, metal translocation and total uptake). Spectroscopic analysis (Fig. S1) was partially supported by the Ministry of Science and Higher Education of the Russian Federation, state assignments № 122042700044–6, 122042700043–9. The authors thank Prof. M. S. Trofimova and N. K. Lapshin for their assistance with measuring the absorption and fluorescence emission spectra and Prof. Hélène Frérot-Pauwels, Université Lille1, for donating the seeds of Arabidopsis lyrata and Arabidopsis halleri.

Publisher Copyright:
© 2023 Elsevier Masson SAS

Funding

This work was supported by the Russian Science Foundation grant № 21-14-00028 ( https://rscf.ru/project/21-14-00028/ ) (studies of Ni and Zn effects on FA profiles, the UI, desaturase activity, and MDA concentration, as well as Ni and Zn accumulation in plant roots and shoots, metal translocation and total uptake). Spectroscopic analysis ( Fig. S1 ) was partially supported by the Ministry of Science and Higher Education of the Russian Federation , state assignments № 122042700044–6 , 122042700043–9 . The authors thank Prof. M. S. Trofimova and N. K. Lapshin for their assistance with measuring the absorption and fluorescence emission spectra and Prof. Hélène Frérot-Pauwels, Université Lille1, for donating the seeds of Arabidopsis lyrata and Arabidopsis halleri. This work was supported by the Russian Science Foundation grant № 21-14-00028 (https://rscf.ru/project/21-14-00028/) (studies of Ni and Zn effects on FA profiles, the UI, desaturase activity, and MDA concentration, as well as Ni and Zn accumulation in plant roots and shoots, metal translocation and total uptake). Spectroscopic analysis (Fig. S1) was partially supported by the Ministry of Science and Higher Education of the Russian Federation, state assignments № 122042700044–6, 122042700043–9. The authors thank Prof. M. S. Trofimova and N. K. Lapshin for their assistance with measuring the absorption and fluorescence emission spectra and Prof. Hélène Frérot-Pauwels, Université Lille1, for donating the seeds of Arabidopsis lyrata and Arabidopsis halleri.

Keywords

Arabidopsis halleri
Arabidopsis lyrata
Fatty acids
Metabolism
Metal hyperaccumulation
Nickel
Oxidative stress
Stress response
Tolerance
Zinc

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.plaphy.2023.107640

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@article{7224d6cfc9be4daa8352e655d5de5884,

title = "Zinc- and nickel-induced changes in fatty acid profiles in the zinc hyperaccumulator Arabidopsis halleri and non-accumulator Arabidopsis lyrata",

abstract = "This pilot study aimed at comparing zinc (Zn) and nickel (Ni) effects on the fatty acid (FA) profiles, oxidative stress and desaturase activity in the Zn hyperaccumulator Arabidopsis halleri and the excluder Arabidopsis lyrata to allow a better picture of the physiological mechanisms which may contribute to metal tolerance or acclimation. The most significant changes in the FA composition were observed in the shoots of the hyperaccumulator and in the roots of the excluder, and were not only metal-dependent, but also species-specific, since the most significant changes in the shoots of A. halleri were observed under Ni treatment, though Ni, in contrast to Zn, was accumulated mainly in its roots. Several FAs appeared in the roots and shoots of A. lyrata only upon metal exposure, whereas they were already found in control A. halleri. In both species, there was an increase in oleic acid under Ni treatment in both organs, whereas in Zn-treated plants the increase was shown only for the shoots. A rare conjugated α-parinaric acid was identified only in the shoots of metal-treated A. halleri. In the shoots of the hyperaccumulator, there was an increase in the content of saturated FAs and a decrease in the content of unsaturated FAs, while in the roots of the excluder, the opposite pattern was observed. These metal-induced changes in FA composition in the shoots of A. halleri can lead to a decrease in the fluidity of membranes, which could diminish the penetration of ROS into the membrane and thus maintain its stability.",

keywords = "Arabidopsis halleri, Arabidopsis lyrata, Fatty acids, Metabolism, Metal hyperaccumulation, Nickel, Oxidative stress, Stress response, Tolerance, Zinc",

author = "Seregin, {Ilya V.} and Ivanova, {Tatiana V.} and Voronkov, {Alexander S.} and Kozhevnikova, {Anna D.} and Henk Schat",

note = "Funding Information: This work was supported by the Russian Science Foundation grant № 21-14-00028 ( https://rscf.ru/project/21-14-00028/ ) (studies of Ni and Zn effects on FA profiles, the UI, desaturase activity, and MDA concentration, as well as Ni and Zn accumulation in plant roots and shoots, metal translocation and total uptake). Spectroscopic analysis ( Fig. S1 ) was partially supported by the Ministry of Science and Higher Education of the Russian Federation , state assignments № 122042700044–6 , 122042700043–9 . The authors thank Prof. M. S. Trofimova and N. K. Lapshin for their assistance with measuring the absorption and fluorescence emission spectra and Prof. H{\'e}l{\`e}ne Fr{\'e}rot-Pauwels, Universit{\'e} Lille1, for donating the seeds of Arabidopsis lyrata and Arabidopsis halleri. Funding Information: This work was supported by the Russian Science Foundation grant № 21-14-00028 (https://rscf.ru/project/21-14-00028/) (studies of Ni and Zn effects on FA profiles, the UI, desaturase activity, and MDA concentration, as well as Ni and Zn accumulation in plant roots and shoots, metal translocation and total uptake). Spectroscopic analysis (Fig. S1) was partially supported by the Ministry of Science and Higher Education of the Russian Federation, state assignments № 122042700044–6, 122042700043–9. The authors thank Prof. M. S. Trofimova and N. K. Lapshin for their assistance with measuring the absorption and fluorescence emission spectra and Prof. H{\'e}l{\`e}ne Fr{\'e}rot-Pauwels, Universit{\'e} Lille1, for donating the seeds of Arabidopsis lyrata and Arabidopsis halleri. Publisher Copyright: {\textcopyright} 2023 Elsevier Masson SAS",

year = "2023",

month = apr,

doi = "10.1016/j.plaphy.2023.107640",

language = "English",

volume = "197",

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Zinc- and nickel-induced changes in fatty acid profiles in the zinc hyperaccumulator Arabidopsis halleri and non-accumulator Arabidopsis lyrata. / Seregin, Ilya V.; Ivanova, Tatiana V.; Voronkov, Alexander S. et al.
In: Plant Physiology and Biochemistry, Vol. 197, 107640, 04.2023, p. 1-21.

Research output: Contribution to Journal › Article › Academic › peer-review

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T1 - Zinc- and nickel-induced changes in fatty acid profiles in the zinc hyperaccumulator Arabidopsis halleri and non-accumulator Arabidopsis lyrata

AU - Seregin, Ilya V.

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AU - Schat, Henk

N1 - Funding Information: This work was supported by the Russian Science Foundation grant № 21-14-00028 ( https://rscf.ru/project/21-14-00028/ ) (studies of Ni and Zn effects on FA profiles, the UI, desaturase activity, and MDA concentration, as well as Ni and Zn accumulation in plant roots and shoots, metal translocation and total uptake). Spectroscopic analysis ( Fig. S1 ) was partially supported by the Ministry of Science and Higher Education of the Russian Federation , state assignments № 122042700044–6 , 122042700043–9 . The authors thank Prof. M. S. Trofimova and N. K. Lapshin for their assistance with measuring the absorption and fluorescence emission spectra and Prof. Hélène Frérot-Pauwels, Université Lille1, for donating the seeds of Arabidopsis lyrata and Arabidopsis halleri. Funding Information: This work was supported by the Russian Science Foundation grant № 21-14-00028 (https://rscf.ru/project/21-14-00028/) (studies of Ni and Zn effects on FA profiles, the UI, desaturase activity, and MDA concentration, as well as Ni and Zn accumulation in plant roots and shoots, metal translocation and total uptake). Spectroscopic analysis (Fig. S1) was partially supported by the Ministry of Science and Higher Education of the Russian Federation, state assignments № 122042700044–6, 122042700043–9. The authors thank Prof. M. S. Trofimova and N. K. Lapshin for their assistance with measuring the absorption and fluorescence emission spectra and Prof. Hélène Frérot-Pauwels, Université Lille1, for donating the seeds of Arabidopsis lyrata and Arabidopsis halleri. Publisher Copyright: © 2023 Elsevier Masson SAS

PY - 2023/4

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N2 - This pilot study aimed at comparing zinc (Zn) and nickel (Ni) effects on the fatty acid (FA) profiles, oxidative stress and desaturase activity in the Zn hyperaccumulator Arabidopsis halleri and the excluder Arabidopsis lyrata to allow a better picture of the physiological mechanisms which may contribute to metal tolerance or acclimation. The most significant changes in the FA composition were observed in the shoots of the hyperaccumulator and in the roots of the excluder, and were not only metal-dependent, but also species-specific, since the most significant changes in the shoots of A. halleri were observed under Ni treatment, though Ni, in contrast to Zn, was accumulated mainly in its roots. Several FAs appeared in the roots and shoots of A. lyrata only upon metal exposure, whereas they were already found in control A. halleri. In both species, there was an increase in oleic acid under Ni treatment in both organs, whereas in Zn-treated plants the increase was shown only for the shoots. A rare conjugated α-parinaric acid was identified only in the shoots of metal-treated A. halleri. In the shoots of the hyperaccumulator, there was an increase in the content of saturated FAs and a decrease in the content of unsaturated FAs, while in the roots of the excluder, the opposite pattern was observed. These metal-induced changes in FA composition in the shoots of A. halleri can lead to a decrease in the fluidity of membranes, which could diminish the penetration of ROS into the membrane and thus maintain its stability.

AB - This pilot study aimed at comparing zinc (Zn) and nickel (Ni) effects on the fatty acid (FA) profiles, oxidative stress and desaturase activity in the Zn hyperaccumulator Arabidopsis halleri and the excluder Arabidopsis lyrata to allow a better picture of the physiological mechanisms which may contribute to metal tolerance or acclimation. The most significant changes in the FA composition were observed in the shoots of the hyperaccumulator and in the roots of the excluder, and were not only metal-dependent, but also species-specific, since the most significant changes in the shoots of A. halleri were observed under Ni treatment, though Ni, in contrast to Zn, was accumulated mainly in its roots. Several FAs appeared in the roots and shoots of A. lyrata only upon metal exposure, whereas they were already found in control A. halleri. In both species, there was an increase in oleic acid under Ni treatment in both organs, whereas in Zn-treated plants the increase was shown only for the shoots. A rare conjugated α-parinaric acid was identified only in the shoots of metal-treated A. halleri. In the shoots of the hyperaccumulator, there was an increase in the content of saturated FAs and a decrease in the content of unsaturated FAs, while in the roots of the excluder, the opposite pattern was observed. These metal-induced changes in FA composition in the shoots of A. halleri can lead to a decrease in the fluidity of membranes, which could diminish the penetration of ROS into the membrane and thus maintain its stability.

KW - Arabidopsis halleri

KW - Arabidopsis lyrata

KW - Fatty acids

KW - Metabolism

KW - Metal hyperaccumulation

KW - Nickel

KW - Oxidative stress

KW - Stress response

KW - Tolerance

KW - Zinc

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Zinc- and nickel-induced changes in fatty acid profiles in the zinc hyperaccumulator Arabidopsis halleri and non-accumulator Arabidopsis lyrata

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

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